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Mef2a (BC096598) Mouse Tagged ORF Clone
CAT＃: MG207872
- TrueORF®
Mef2a (tGFP-tagged) - Mouse myocyte enhancer factor 2A (cDNA clone MGC:106070 IMAGE:4979487)
ORF Plasmid: DDK
"BC096598" in other vectors (6)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A430079H05Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207872 representing BC096598
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGAAAGAAGATACAAATCACACGCATAATGGATGAGAGGAACCGACAGGTTACTTTTACAAAGC GAAAGTTTGGATTGATGAAGAAAGCCTATGAACTCAGTGTGCTCTGTGACTGTGAAATAGCACTCATCAT TTTCAACAGCTCTAATAAGCTGTTTCAGTACGCTAGCACTGACATGGACAAAGTCCTTCTCAAATACACT GAGTATAACGAGCCTCATGAAAGCAGGACGAACTCGGATATCGTTGAGGCTCTGAACAAGAAGGAACACA GAGGGTGCGACAGCCCAGACCCTGATACTTCATATGTGCTAACTCCACATACAGAAGAAAAATATAAAAA AATTAATGAGGAATTTGATAATATGATGCGGAATCATAAAATCGCACCTGGCTTGCCACCTCAGAACTTC TCAATGTCTGTCACAGTCCCTGTGACCAGCCCTAATGCTTTGTCGTACACTAACCCAGGGAGTTCACTCG TGTCACCGTCTTTGGCAGCCAGCTCAACATTAGCAGATTCGAGCATGCTGTCTCCACCCCCAGCCACGCT ACATAGAAATGTGTCCCCAGGAGCTCCGCAGAGACCACCAAGTACCGGCAGTGCAAGTGGGATGTTGAGC ACTACAGACCTCACGGTGCCAAATGGAGCTGGAAATAGTCCTGTGGGGAATGGATTTGTAAACTCAAGGG CCTCTCCAAATTTGATTGGAAATACTGGTGCAAATAGTTTAGGCAAAGTCATGCCTACAAAGTCTCCCCC GCCACCAGGTGGTGGCAGTCTTGGAATGAACAGTCGGAAACCAGATCTACGAGTTGTCATTCCCCCATCA AGCAAGGGCATGATGCCCCCACTTAATGCCCAAAGGATAAGCAGTTCTCAAGCCACTCAACCTCTTGCCA CGCCTGTAGTGTCTGTGACAACTCCGAGCTTGCCTCCACAGGGACTAGTGTACTCAGCAATGCCGACAGC CTACAACACTGATTACTCACTGACCAGCGCTGACCTGTCTGCCCTGCAAGGCTTTACTTCCCCTGGAATG CTGTCTCTGGGGCAGGCTTCAGCCTGGCAGCAGCACCATCTAGGACAAGCAGCCCTCAGCTCTCTGGTGG CTGGAGGGCAGTTATCTCAGGGTTCAAATTTGTCCATTAACACCAACCAGAACATCAACATTAAGTCAGA GCCAATTTCACCTCCCCGTGATAGAATGACCCCATCAGGCTTCCAACAGCAGCAGCAGCAGCCACAACAG CAGCCACCGCCACAACCTCCACAGCCCCAACCCCGGCAGGAAATGGGGCGCTCCCCTGTGGACAGTCTGA GCAGCTCCAGCAGCTCCTATGATGGCAGTGACCGGGAGGATCCACGGGGTGACTTCCATTCTCCAATTGT GCTTGGCCGACCTCCAAATACTGAGGACAGAGAAAGCCCTTCTGTAAAACGCATGAGGATGGACACATGG GTGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207872 representing BC096598
Red=Cloning site Green=Tags(s) MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYT EYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHTEEKYKKINEEFDNMMRNHKIAPGLPPQNF SMSVTVPVTSPNALSYTNPGSSLVSPSLAASSTLADSSMLSPPPATLHRNVSPGAPQRPPSTGSASGMLS TTDLTVPNGAGNSPVGNGFVNSRASPNLIGNTGANSLGKVMPTKSPPPPGGGSLGMNSRKPDLRVVIPPS SKGMMPPLNAQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTSADLSALQGFTSPGM LSLGQASAWQQHHLGQAALSSLVAGGQLSQGSNLSINTNQNINIKSEPISPPRDRMTPSGFQQQQQQPQQ QPPPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKRMRMDTW VT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC096598 |
| ORF Size | 1478 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC096598, AAH96598 |
| RefSeq Size | 2469 bp |
| RefSeq ORF | 1478 bp |
| Locus ID | 17258 |
| Gene Summary | Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217719 | Mef2a (untagged) - Mouse myocyte enhancer factor 2A (cDNA clone MGC:106070 IMAGE:4979487), (10ug) |
CNY 6,940.00 |
|
| MR207872 | Mef2a (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2A (cDNA clone MGC:106070 IMAGE:4979487) |
CNY 5,488.00 |
|
| MR207872L1 | Lenti ORF clone of Mef2a (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2A (cDNA clone MGC:106070 IMAGE:4979487) |
CNY 4,750.00 |
|
| MR207872L2 | Lenti ORF clone of Mef2a (mGFP-tagged) - Mouse myocyte enhancer factor 2A (cDNA clone MGC:106070 IMAGE:4979487) |
CNY 7,888.00 |
|
| MR207872L3 | Lenti ORF clone of Mef2a (Myc-DDK-tagged) - Mouse myocyte enhancer factor 2A (cDNA clone MGC:106070 IMAGE:4979487) |
CNY 4,750.00 |
|
| MR207872L4 | Lenti ORF clone of Mef2a (mGFP-tagged) - Mouse myocyte enhancer factor 2A (cDNA clone MGC:106070 IMAGE:4979487) |
CNY 4,750.00 |
