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Traf2 (NM_009422) Mouse Tagged ORF Clone
CAT＃: MG208047
- TrueORF®
Traf2 (tGFP-tagged) - Mouse Tnf receptor-associated factor 2 (Traf2)
ORF Plasmid: DDK
"NM_009422" in other vectors (6)
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CNY 7,208.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI325259 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208047 representing NM_009422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGCCAGTGTGACTTCCCCTGGCTCCCTAGAACTGCTACAGCCTGGCTTCTCCAAGACCCTCC TGGGGACCAGGTTAGAAGCCAAGTACCTCTGTTCAGCCTGCAAAAACATCCTGCGGAGGCCTTTCCAGGC CCAGTGTGGGCACCGCTACTGCTCCTTCTGCCTGACCAGCATCCTCAGCTCTGGGCCCCAGAACTGTGCT GCCTGTGTCTATGAAGGCCTGTATGAAGAAGGCATTTCTATTTTAGAGAGTAGTTCGGCCTTTCCAGATA ACGCTGCCCGCAGAGAGGTGGAGAGCCTGCCAGCTGTCTGTCCCAATGATGGATGCACTTGGAAGGGGAC CTTGAAAGAATACGAGAGCTGCCACGAAGGACTTTGCCCATTCCTGCTGACGGAGTGTCCTGCATGTAAA GGCCTGGTCCGCCTCAGCGAGAAGGAGCACCACACTGAGCAGGAATGCCCCAAAAGGAGCCTGAGCTGCC AGCACTGCAGAGCACCCTGTAGCCACGTGGACCTGGAGGTACACTATGAGGTCTGCCCCAAGTTTCCCTT AACCTGTGATGGCTGTGGCAAGAAGAAGATCCCTCGGGAGACGTTTCAGGACCATGTTAGAGCATGCAGC AAATGCCGGGTTCTCTGCAGATTCCACACCGTTGGCTGTTCAGAGATGGTGGAGACTGAGAACCTGCAGG ATCATGAGCTGCAGCGGCTACGGGAACACCTAGCCCTACTGCTGAGCTCATTCCTGGAGGCCCAAGCCTC TCCAGGAACCTTGAACCAGGTGGGGCCAGAGCTACTCCAGCGGTGCCAGATTTTGGAGCAGAAGATAGCA ACCTTTGAGAACATTGTCTGCGTCTTGAACCGTGAAGTAGAGAGGGTAGCAGTGACTGCAGAGGCTTGTA GCCGGCAGCACCGGCTAGACCAGGACAAGATTGAGGCCCTGAGTAACAAGGTGCAACAGCTGGAGAGGAG CATCGGCCTCAAGGACCTGGCCATGGCTGACCTGGAGCAGAAGGTCTCCGAGTTGGAAGTATCCACCTAT GATGGGGTCTTCATCTGGAAGATCTCTGACTTCACCAGAAAGCGTCAGGAAGCCGTAGCTGGCCGGACAC CGGCTATCTTCTCCCCAGCCTTCTACACAAGCAGATATGGCTACAAGATGTGTCTACGAGTCTACTTGAA TGGCGACGGCACCGGGCGGGGAACCCATCTGTCTCTCTTCTTCGTGGTGATGAAAGGCCCCAATGATGCT CTGTTGCAGTGGCCTTTTAATCAGAAGGTAACATTGATGTTGCTGGACCATAACAACCGGGAGCATGTGA TCGACGCATTCAGGCCCGATGTAACCTCGTCCTCCTTCCAGAGGCCTGTCAGTGACATGAACATCGCCAG TGGCTGCCCCCTCTTCTGCCCTGTGTCCAAGATGGAGGCCAAGAATTCCTATGTGCGGGATGATGCGATC TTCATCAAAGCTATTGTGGACCTAACAGGACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208047 representing NM_009422
Red=Cloning site Green=Tags(s) MAAASVTSPGSLELLQPGFSKTLLGTRLEAKYLCSACKNILRRPFQAQCGHRYCSFCLTSILSSGPQNCA ACVYEGLYEEGISILESSSAFPDNAARREVESLPAVCPNDGCTWKGTLKEYESCHEGLCPFLLTECPACK GLVRLSEKEHHTEQECPKRSLSCQHCRAPCSHVDLEVHYEVCPKFPLTCDGCGKKKIPRETFQDHVRACS KCRVLCRFHTVGCSEMVETENLQDHELQRLREHLALLLSSFLEAQASPGTLNQVGPELLQRCQILEQKIA TFENIVCVLNREVERVAVTAEACSRQHRLDQDKIEALSNKVQQLERSIGLKDLAMADLEQKVSELEVSTY DGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDA LLQWPFNQKVTLMLLDHNNREHVIDAFRPDVTSSSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAI FIKAIVDLTGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009422 |
| ORF Size | 1503 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009422.2, NP_033448.2 |
| RefSeq Size | 2998 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 22030 |
| UniProt ID | P39429 |
| Gene Summary | Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Isoform 2 does not seem to mediate activation of NF-kappa-B but inhibits isoform 1 activity. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200118 | Traf2 (untagged) - Mouse TNF receptor-associated factor 2 (Traf2), (10ug) |
CNY 5,608.00 |
|
| MR208047 | Traf2 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 2 (Traf2) |
CNY 5,608.00 |
|
| MR208047L1 | Lenti ORF clone of Traf2 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 2 (Traf2) |
CNY 6,940.00 |
|
| MR208047L2 | Lenti ORF clone of Traf2 (mGFP-tagged) - Mouse TNF receptor-associated factor 2 (Traf2) |
CNY 8,008.00 |
|
| MR208047L3 | Lenti ORF clone of Traf2 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 2 (Traf2) |
CNY 6,940.00 |
|
| MR208047L4 | Lenti ORF clone of Traf2 (mGFP-tagged) - Mouse TNF receptor-associated factor 2 (Traf2) |
CNY 6,940.00 |
