United States
Germany
Japan
United Kingdom
China
Fzd1 (NM_021457) Mouse Tagged ORF Clone
CAT＃: MG209705
- TrueORF®
Fzd1 (tGFP-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1)
ORF Plasmid: DDK
"NM_021457" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW227548; FZ-1; Fz1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209705 representing NM_021457
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGGAGGCGGCGCCTAGCGAGTCCCGGGCCGCCGGCCGGCTGAGCTTGGAACTTTGTGCCGAAG CACTCCCGGGCCGGCGGGAGGAGGTGGGGCACGAGGACACGGCCAGCCACCGCCGCCCCCGGGCTGATCC CCGGCGTTGGGCTAGCGGGCTGCTGCTGCTGCTTTGGTTGCTGGAGGCTCCTCTGCTTTTGGGGGTCCGA GCGCAGGCGGCGGGCCAGGTATCCGGGCCGGGCCAGCAAGCCCCGCCGCCGCCCCAGCCCCAGCAGAGCG GGCAGCAGTACAACGGCGAACGGGGCATCTCCATCCCGGACCACGGCTACTGCCAGCCCATCTCCATCCC GCTGTGCACGGACATCGCGTACAACCAGACCATCATGCCCAACCTGCTGGGCCACACGAATCAGGAGGAC GCCGGTCTGGAGGTGCACCAGTTCTACCCTCTGGTGAAGGTGCAGTGCTCCGCCGAGCTCAAGTTCTTCC TGTGCTCCATGTACGCGCCTGTGTGCACCGTACTGGAGCAGGCGCTACCGCCCTGCCGCTCCCTGTGCGA GCGCGCACGCCAGGGCTGCGAGGCGCTCATGAACAAGTTCGGCTTCCAGTGGCCAGACACACTCAAGTGC GAGAAGTTCCCGGTGCACGGCGCAGGAGAGCTGTGCGTGGGCCAGAACACGTCCGACAAAGGCACCCCAA CTCCCTCCTTGCTACCAGAGTTCTGGACCAGTAATCCGCAGCACGGCGGCGGTGGTTACCGCGGCGGCTA CCCGGGGGGTGCCGGGACGGTGGAGCGGGGAAAGTTCTCCTGCCCGCGCGCCCTCAGGGTGCCCTCCTAC CTCAACTACCACTTTCTGGGGGAGAAGGACTGCGGCGCACCCTGCGAACCCACCAAGGTTTACGGGCTCA TGTACTTCGGGCCAGAGGAGCTGCGCTTCTCGCGCACCTGGATAGGCATCTGGTCCGTGCTGTGCTGCGC CTCCACGCTCTTCACGGTGCTCACGTACCTAGTGGACATGCGGCGCTTCAGCTACCCGGAACGGCCCATC ATTTTCCTGTCCGGCTGTTACACAGCGGTGGCGGTGGCCTACATCGCTGGCTTTCTGTTGGAGGACCGGG TGGTGTGCAACGACAAGTTTGCAGAGGACGGGGCGCGCACGGTGGCGCAGGGCACTAAGAAAGAAGGCTG CACTATACTCTTTATGATGCTCTACTTCTTCAGCATGGCCAGCTCCATCTGGTGGGTGATCCTGTCCCTC ACCTGGTTCCTGGCAGCCGGCATGAAGTGGGGCCACGAAGCCATCGAGGCCAACTCACAGTATTTCCATT TAGCCGCCTGGGCTGTGCCAGCCATCAAAACTATAACCATCTTGGCGTTGGGCCAGGTGGATGGCGACGT ACTGAGCGGAGTGTGTTTTGTGGGGCTCAACAACGTGGACGCACTGCGTGGCTTTGTGCTGGCGCCTCTC TTCGTTTATCTGTTCATTGGCACTTCTTTCCTGCTGGCCGGTTTCGTGTCACTCTTCCGCATCCGCACCA TCATGAAGCATGACGGCACCAAGACAGAGAAGCTGGAGAAGCTCATGGTGCGCATCGGAGTCTTCAGTGT CCTCTACACTGTGCCGGCCACCATCGTCATCGCCTGCTACTTCTATGAACAGGCCTTTCGGGACCAGTGG GAGCGCAGCTGGGTGGCCCAGAGCTGCAAGAGTTATGCCATCCCTTGCCCTCACCTCCAGGGAGGTGGAG GAGTCCCACCACACCCGCCCATGAGCCCAGACTTTACAGTCTTCATGATCAAGTATCTCATGACGCTGAT TGTGGGCATCACATCGGGCTTCTGGATCTGGTCCGGCAAGACACTGAATTCCTGGAGGAAGTTCTACACG AGGCTTACCAACAGCAAACAGGGGGAGACTACCGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209705 representing NM_021457
Red=Cloning site Green=Tags(s) MAEEAAPSESRAAGRLSLELCAEALPGRREEVGHEDTASHRRPRADPRRWASGLLLLLWLLEAPLLLGVR AQAAGQVSGPGQQAPPPPQPQQSGQQYNGERGISIPDHGYCQPISIPLCTDIAYNQTIMPNLLGHTNQED AGLEVHQFYPLVKVQCSAELKFFLCSMYAPVCTVLEQALPPCRSLCERARQGCEALMNKFGFQWPDTLKC EKFPVHGAGELCVGQNTSDKGTPTPSLLPEFWTSNPQHGGGGYRGGYPGGAGTVERGKFSCPRALRVPSY LNYHFLGEKDCGAPCEPTKVYGLMYFGPEELRFSRTWIGIWSVLCCASTLFTVLTYLVDMRRFSYPERPI IFLSGCYTAVAVAYIAGFLLEDRVVCNDKFAEDGARTVAQGTKKEGCTILFMMLYFFSMASSIWWVILSL TWFLAAGMKWGHEAIEANSQYFHLAAWAVPAIKTITILALGQVDGDVLSGVCFVGLNNVDALRGFVLAPL FVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLEKLMVRIGVFSVLYTVPATIVIACYFYEQAFRDQW ERSWVAQSCKSYAIPCPHLQGGGGVPPHPPMSPDFTVFMIKYLMTLIVGITSGFWIWSGKTLNSWRKFYT RLTNSKQGETTV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021457 |
| ORF Size | 4392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021457.3, NP_067432.2 |
| RefSeq Size | 4150 bp |
| RefSeq ORF | 1929 bp |
| Locus ID | 14362 |
| UniProt ID | O70421 |
| Gene Summary | Receptor for Wnt proteins (PubMed:15923619). Activated by WNT7B (PubMed:15923619). Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B (By similarity). Contradictory results showing activation by WNT7B have been described for mouse (PubMed:15923619). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:15923619). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:15923619). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203642 | Fzd1 (untagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1), (10ug) |
CNY 2,000.00 |
|
| MR209705 | Fzd1 (Myc-DDK-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1) |
CNY 4,560.00 |
|
| MR209705L3 | Lenti ORF clone of Fzd1 (Myc-DDK-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1) |
CNY 6,460.00 |
|
| MR209705L4 | Lenti ORF clone of Fzd1 (mGFP-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1) |
CNY 6,460.00 |
