Fzd1 (NM_021457) Mouse Tagged Lenti ORF Clone
CAT#: MR209705L3
- LentiORF®
Lenti ORF clone of Fzd1 (Myc-DDK-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1)
"NM_021457" in other vectors (4)
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CNY 6,460.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AW227548; FZ-1; Fz1 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR209705).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021457 |
ORF Size | 1926 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021457.3, NP_067432.2 |
RefSeq Size | 4395 bp |
RefSeq ORF | 1929 bp |
Locus ID | 14362 |
UniProt ID | O70421 |
Gene Summary | Receptor for Wnt proteins (PubMed:15923619). Activated by WNT7B (PubMed:15923619). Activated by WNT3A, WNT3, WNT1 and to a lesser extent WNT2, but apparently not by WNT4, WNT5A, WNT5B, WNT6, WNT7A or WNT7B (By similarity). Contradictory results showing activation by WNT7B have been described for mouse (PubMed:15923619). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:15923619). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed:15923619). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203642 | Fzd1 (untagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1), (10ug) |
CNY 2,000.00 |
|
MG209705 | Fzd1 (tGFP-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1) |
CNY 5,040.00 |
|
MR209705 | Fzd1 (Myc-DDK-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1) |
CNY 4,560.00 |
|
MR209705L4 | Lenti ORF clone of Fzd1 (mGFP-tagged) - Mouse frizzled homolog 1 (Drosophila) (Fzd1) |
CNY 6,460.00 |