Ski (BC068305) Mouse Tagged ORF Clone
CAT＃: MG209957
- TrueORF®
Ski (tGFP-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626)
ORF Plasmid: DDK
"BC068305" in other vectors (4)
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CNY 4280.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC8300 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209957 representing BC068305
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCTGCAGCGGCCGGCCGGGGCGGCTTCCAGCCGCACCCGGGGCTGCAGAAGACGCTGGAGCAGT TCCACCTGAGCTCCATGAGCTCGCTGGGCGGCCCGGCCGCTTTCTCGGCGCGCTGGGCGCAGGAGGCCTA CAAGAAGGAGACCGTGCTGGAAGGGGAGACCATCTCTTGTTTCGTGGTGGGAGGTGAGAAGCGTCTGTGC CTGCCGCAGATCCTCAACTCGGTGCTGCGCGACTTCTCACTGCAGCAGATCAACTCCGTGTGCGATGAGC TGCACATCTACTGCTCGCGCTGCACCGCCGACCAGCTGGAGATCCTCAAAGTCATGGGCATCCTGCCCTT CTCCGCGCCCTCCTGCGGGCTCATCACCAAGACGGACGCCGAGCGCCTGTGCAACGCGCTGCTCTACGGC GGCGCCTACCCGCCGCCTTGCAAGAAGAAGTTGGCGGCCAGCCTGGCGCTGGGCCTGGAGCTCAGCGAAC GCAGCGTCCGCGTGTACCACGAGTGCTTCGGCAAGTGCAAAGGGCTGCTGGTGCCCGAGCTGTACAGCAG TCCCAGCGCTGCCTGCATCCAGTGCCTCGACTGCCGCCTCATGTACCCGCCGCACAAGTTCGTTGTACAC TCACACAAGGCCCTGGAGAACCGGACCTGCCACTGGGGCTTCGACTCAGCCAACTGGCGGGCCTACATCC TGCTGAGCCAGGACTACACGGGCAAGGAGGAGCAGGCCCGCCTTGGCCGCTGCCTGGACGACGTGAAGGA GAAGTTCGACTATGCCAACAAGTACAAGCGGAGGGTGCCCCGGGTCTCAGAGCCCCCAGCTTCCATAAGA CCCAAAACAGACGACACTTCCTCACAGTCCCCTGCGTCTTCTGAGAAGGACAAGCAGTCCACTTGGCTGC GGACCTTGGCTGGTTCCTCCAATAAGAGCCTCGGCTGCACTCACCCTCGCCAGCGCCTTTCTGCTTTCCG GCCCTGGTCCCCTGCAGTGTCTGCGAGTGAGAAAGAGACGTCCCCACACCTCCCAGCTTTGATTCGAGAC AGCTTCTACTCCTACAAGAGCTTTGAGACAGCCGTGGCCCCCAATGTGGCCCTAGCACCACCAACCCAGC AAAAGGTTGTGAACAGTCCACCCTGCACCACCGTGGTCTCCCGGGCACCAGAGCCTCTTACTACTTGTAT CCAGCCACGAAAACGGAAGCTGACTCTGGACACAGCAGGAGCCCCAGACATGCTGACACCTGTGGCTGCT GCAGAGGAAGACAAGGATTCTGAGGCCGAGGTTGAAGTTGAAAGCAGAGAGGAGTTCACCTCCTCCTTGT CCTCGCTGTCCTCTCCGTCCTTTACCTCGTCCAGCTCTGCCAAGGATCTAAGCTCCCCAGGCATGCATGC TCCGCCCGTGGTTGCCCCCGATGCTGCAGCCCATGTTGATGCCCCAAGTGGGCTGGAGGCAGAGTTGGAG CACCTGCGGCAAGCCCTGGAGGGTGGCCTGGATACTAAGGAAGCCAAAGAAAAGTTCCTGCATGAGGTGG TCAAAATGCGAGTTAAGCAAGAGGAAAAGCTGACAGCAGCCCTGCAGGCTAAGCGCAGCCTGCACCAGGA GCTAGAATTCTTGCGTGTGGCCAAGAAGGAGAAACTGCGGGAAGCCACAGAGGCCAAGCGCAGTTTGAGG AAAGAGATTGAGCGGCTCCGAGCTGAAAACGAGAAGAAGATGAAAGAGGCCAACGAGTCCCGGGTGCGCC TGAAGCGAGAGCTGGAGCAGGCGCGGCAGGTCCGGGTGTGTGACAAGGGCTGCGAGGCCGGGCGCCTGCG TGCCAAGTACTCAGCCCAGATTGAGGACCTGCAAGCGAAGCTGCAGCATGCGGAGGCCGACCGCGAGCAG CTTCGAGCTGACCTGCTGCGGGAACGTGAGGCCCGCGAGCATCTGGAGAAGGTGGTGAGGGAGCTTCAGG AGCAGCTGCGGCCTCGGCCACGGCCTGAGCATCCTGGAGGTGAGAGCAACGCTGAGCTGGGTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209957 representing BC068305
Red=Cloning site Green=Tags(s) MEAAAAGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKETVLEGETISCFVVGGEKRLC LPQILNSVLRDFSLQQINSVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYG GAYPPPCKKKLAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVH SHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYANKYKRRVPRVSEPPASIR PKTDDTSSQSPASSEKDKQSTWLRTLAGSSNKSLGCTHPRQRLSAFRPWSPAVSASEKETSPHLPALIRD SFYSYKSFETAVAPNVALAPPTQQKVVNSPPCTTVVSRAPEPLTTCIQPRKRKLTLDTAGAPDMLTPVAA AEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLSSPGMHAPPVVAPDAAAHVDAPSGLEAELE HLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLTAALQAKRSLHQELEFLRVAKKEKLREATEAKRSLR KEIERLRAENEKKMKEANESRVRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQAKLQHAEADREQ LRADLLREREAREHLEKVVRELQEQLRPRPRPEHPGGESNAELGP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC068305 |
| ORF Size | 2025 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068305, AAH68305 |
| RefSeq Size | 3912 bp |
| RefSeq ORF | 2027 bp |
| Locus ID | 20481 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220756 | Ski (untagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626), (10ug) |
CNY 9120.00 |
|
| MR209957 | Ski (Myc-DDK-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626) |
CNY 4944.00 |
|
| MR209957L3 | Lenti ORF clone of Ski (Myc-DDK-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626) |
CNY 5800.00 |
|
| MR209957L4 | Lenti ORF clone of Ski (mGFP-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626) |
CNY 7344.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
