Sec14l1 (BC005766) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1200017E04Rik; 2810012L19Rik; Naa-35
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210239 representing BC005766 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAAAAATACCAGTCCCCAGTGCGGGTGTATAAACATCCCTTTGAGTTAATTATGGCTGCCTATG AGAGGAGGTTTCCTACATGTCCTCTGATTCCAATGTTCGTGGACAGTGACACTGTGAGCGAGTTCAAGAG TGAGGATGGGGCTCTTCATGTCATCGAGAGGCGCTGCAAGCTGGATATAGATGCGCCCAGGCTGTTGAAG AAGATCGCAGGAGTCGATTACGTTTATTTTGTCCAGAAAAACTCCCTGAATTCTCGAGATCGCACTTTGC ACATCGAGGCCCACAATGAGACATTTTCTAACCGGGTCATCATCCACGAACACTGCTGTTACACGGTTCA CCCTGAGAATGAAGACTGGACCTGTTTCGAACAGTCTGCAAGTTTAGATATCAAATCCTTCTTTGGTTTT GAAAGTACAGTGGAAAAAATTGCCATGAAACATTATACAAGCAACATTAAAAAAGGGAAGGAGATCATTG AATACTACCTACGGCAGCTGGAGGAGGAAGGCATCACCTTCGTGCCCCGCTGGACCCCACCCCCCGTGGG ACCCTCAGAAACATGTTCATCAAGCAAGAATCAAGTCACATCTGCAGCTGTCCTTGTCCCAGATGCTGCT GCCGTCATGGAGGGGCTGAGTGGGGAAAACCTCAGTAGTCCAGGAACAGCGTCTGAGCCCGTGGTGGGAA CCCCTGACGATAAATTAGATGCCGACTACATCAAGAGATACTTGGGTGACCTGACTCCCCTCCAAGAGAG CTGCCTCATTCGACTGCGCCAGTGGCTCCAGGAGACACACAAGGGCAAGATCCCAAAGGACGAGCATATC CTTCGGTTCCTGCGTGCCCGGGATTTTAATATTGACAAGGCCAGAGAGATCATGTGCCAGTCTCTAACCT GGCGGAAGCAGCACCAGGTGGATTACATCCTGGACACTTGGACTCCGCCCCAGGTCCTTCTGGACTACTA CGCTGGGGGCTGGCATCACCATGACAAAGATGGACGGCCGCTGTATGTGCTTAGGCTGGGCCAGATGGAC ACCAAAGGCTTGGTGCGAGCTCTCGGAGAGGAGGCCCTACTGCGATACGTTCTTTCCATAAACGAAGAAG GCCTGAGACGATGCGAGGAAAACACCAAGGTCTTCGGCCGGCCAATCAGCTCGTGGACCTGCTTGGTGGA CCTTGAAGGACTGAACATGCGCCATCTATGGAGACCAGGGGTCAAAGCCTTGCTGCGCATCATCGAGGTG GTGGAGGCCAACTACCCGGAGACGCTGGGCCGCCTCCTCATCCTCCGAGCCCCCAGGGTCTTCCCTGTCC TCTGGACGCTGGTTAGTCCATTTATTGATGACAACACCAGAAGGAAGTTCCTCATTTATGCAGGAAATGA CTACCAGGGCCCAGGAGGCCTGCTGGATTACATCGATAAAGAGATTATTCCCGATTTCCTGAGTGGGGAG TGCATGTGTGACGTGCCAGAGGGTGGATTGGTCCCCAAATCCCTGTACAGGACTGCAGAGGAGCTGGAAA ATGAAGACCTGAAGCTCTGGACGGAGACCATCTACCAGTCTGCCAGCGTGTTCAAAGGAGCTCCACATGA GATTCTCATTCAGATTGTGGACGCCTCCTCAGTGATCACCTGGGATTTTGATGTGTGCAAAGGGGACATC GTCTTTAACATTTATCACTCCAAGAGGTCTCCCCAGCCACCCAAGAAGGACTCGCTAGGGGCTCACAGCA TCACTTCCCCAGGAGGCAACAACGTGCAGCTGATAGACAAAGTCTGGCAGCTGGGCCGGGACTATAGCAT GGTGGAGTCTCCTCTAATCTGCAAAGAAGGAGAGAGCGTGCAGGGGTCCCATGTGACCAGGTGGCCAGGC TTCTACATCCTACAGTGGAAATTCCACACCATGCCAGCATGTGCTGCCACCAACCTACCCCGAGTGGACG ACGTGCTGGCCTCCCTGCAGGTCTCCTCCCACAAGTGCAAAGTCATGTACTACACAGAAGTGATCGGCTC TGAAGATTTCAGAGGCTCCATGACCAGCCTGGAATCTAGCCACAGCGGGTTCTCACAGCTCAGTGCCGCC ACCACCTCCTCCAGCCAGTCGCAATCCAGCTCCATGATTTCCAGTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210239 representing BC005766 Red=Cloning site Green=Tags(s) MVQKYQSPVRVYKHPFELIMAAYERRFPTCPLIPMFVDSDTVSEFKSEDGALHVIERRCKLDIDAPRLLK KIAGVDYVYFVQKNSLNSRDRTLHIEAHNETFSNRVIIHEHCCYTVHPENEDWTCFEQSASLDIKSFFGF ESTVEKIAMKHYTSNIKKGKEIIEYYLRQLEEEGITFVPRWTPPPVGPSETCSSSKNQVTSAAVLVPDAA AVMEGLSGENLSSPGTASEPVVGTPDDKLDADYIKRYLGDLTPLQESCLIRLRQWLQETHKGKIPKDEHI LRFLRARDFNIDKAREIMCQSLTWRKQHQVDYILDTWTPPQVLLDYYAGGWHHHDKDGRPLYVLRLGQMD TKGLVRALGEEALLRYVLSINEEGLRRCEENTKVFGRPISSWTCLVDLEGLNMRHLWRPGVKALLRIIEV VEANYPETLGRLLILRAPRVFPVLWTLVSPFIDDNTRRKFLIYAGNDYQGPGGLLDYIDKEIIPDFLSGE CMCDVPEGGLVPKSLYRTAEELENEDLKLWTETIYQSASVFKGAPHEILIQIVDASSVITWDFDVCKGDI VFNIYHSKRSPQPPKKDSLGAHSITSPGGNNVQLIDKVWQLGRDYSMVESPLICKEGESVQGSHVTRWPG FYILQWKFHTMPACAATNLPRVDDVLASLQVSSHKCKVMYYTEVIGSEDFRGSMTSLESSHSGFSQLSAA TTSSSQSQSSSMISSE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	BC005766
ORF Size	2148 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005766, AAH05766
RefSeq Size	2628 bp
RefSeq ORF	2150 bp
Locus ID	74136
Gene Summary	May play a role in innate immunity by inhibiting the antiviral RIG-I signaling pathway. In this pathway, functions as a negative regulator of DDX58/RIG-I, the cytoplasmic sensor of viral nucleic acids. Prevents the interaction of DDX58 with MAVS/IPS1, an important step in signal propagation. May also regulate the SLC18A3 and SLC5A7 cholinergic transporters.[UniProtKB/Swiss-Prot Function]

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