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Bap1 (NM_027088) Mouse Tagged ORF Clone
CAT＃: MG210327
- TrueORF®
Bap1 (tGFP-tagged) - Mouse Brca1 associated protein 1 (Bap1)
ORF Plasmid: DDK
"NM_027088" in other vectors (4)
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CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2300006C11Rik; AA989761; AW553466; mKIAA0272; uch-x4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210327 representing NM_027088
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAAGGGCTGGCTGGAGCTGGAGAGTGACCCAGGCCTCTTCACCCTCCTGGTGGAAGATTTCGGTG TCAAAGGGGTGCAAGTGGAGGAGATCTATGACCTTCAGAGTAAATGCCAGGGGCCTGTGTATGGATTTAT CTTCCTGTTCAAATGGATCGAAGAGCGCAGGTCCCGCCGCAAGGTTTCTACGTTGGTGGATGACACGTCT GTGATTGATGATGATATTGTGAATAACATGTTCTTTGCTCACCAGCTGATTCCCAACTCTTGTGCCACTC ACGCCTTGCTAAGCGTGCTTCTGAACTGCAGCAATGTGGATCTGGGGCCCACGCTGAGCCGAATGAAGGA TTTCACCAAAGGCTTCAGTCCTGAGAGCAAAGGATATGCAATTGGCAATGCCCCTGAACTGGCCAAGGCA CATAATAGCCATGCCAGGCCAGAACCACGTCACCTTCCTGAGAAACAGAATGGCCTCAGTGCAGTGCGGA CCATGGAGGCGTTTCATTTTGTCAGCTATGTGCCTATCACAGGGAGGCTCTTTGAATTGGATGGATTGAA AGTCTACCCAATTGATCATGGGCCCTGGGGAGAGGACGAGGAGTGGACAGATAAAGCTCGAAGGGTCATC ATGGAGCGAATCGGTCTTGCTACTGCAGGGGAGCCCTATCATGACATTCGCTTCAACCTGATGGCAGTGG TGCCTGACCGCAGGATTAAGTATGAGACCAGGCTACATGTACTGAAGGTGAACCGACAAACAGTCCTGGA GGCCCTGCAGCAGCTGATTAGAGTAACCCAGCCGGAGCTGATTCAGACCCACAAATCTCAAGAGTCACAG CTGCCTGAGGAGAGCAAGCCAGCCAGCAGCAAGTCCCCCCTTGGACTGGAGGCAGGCAGAACCCCAGTGG CCTCTGAGTGCACTCAGACAGATGGTGCAGAGGAGGTGGCTGGTTCGTGCCCACAAACTACAACCCATAG TCCTCCCAGCAAATGTAAGCTGGTGGTGAAGCCTCCAGGGAGCAGCCTCAATGGGGTTCCCCCAAACCCT GCCCCTATTGTCCAGCGGCTGCCAGCCTTCCTAGACAATCACAATTATGCCAAATCCCCTATGCAGGAGG AGGAAGACCTGGCGGCAGGAGTGGGTCGCAGCCGCGTTCCCGTCCGAGCGCCCCAGCAGTACTCTGAAGA CGAGGATGACTACGAGGATGAGGACGAGGACGTGCAGAACACCAACCCTGCCATCAGATACAAGCGGAAG GGGACAGGGAAGCCAGGATCGTTGAGCAATTCTTCAGATGGGCAGCTGTCAGTGCTGCAGCCCAACACCA TCAATGTCTTAACTGAGAAGCTTCAAGAGTCTCAGAAAGACCTTTCAGTTCCTCTGTCCATCAAGACTAG CAGTGGGGCTGGGAGTCCAGCTGTGGCTGTGCCCACACACTCGCAACCTTCACCCACCCCTAGCAATGAG AGCACGGACACAGCCTCTGAGATTGGCAGTGCTTTCAACTCACCCTTGCGCTCGCCCATCCGCTCGGCCA ACCCAACACGGCCCTCTAGCCCTGTCACCTCTCACATCTCCAAGGTGCTTTTTGGAGAAGATGACAGCCT ACTTCGTGTTGACTGCATACGCTACAACCGTGCTGTCCGTGACCTGGGTCCTGTCATTAGCACGGGCCTG CTGCACCTTGCTGAAGATGGTGTACTGAGTCCCCTGGCACTCACAGAGGGTGGGAAGGGTTCCTCACCTT CTACCAGATCAAGCCAAGGCAGCCAGGGGTCCAGTGGCCTAGAGGAGAAGGAGGTGGTGGAAGTCACAGA GAGCAGAGACAAGCCTGGGCTGAACAGGTCCAGTGAGCCCTTGAGTGGAGAGAAGTACTCACCCAAGGAG CTGCTAGCACTGCTAAAGTGTGTAGAAGCCGAGATTGCAAACTATGAGGCCTGTCTCAAGGAGGAGGTGG AGAAAAGGAAGAAGTTCAAGATTGATGACCAGCGAAGGACCCACAACTATGACGAGTTTATCTGTACCTT CATATCCATGCTGGCTCAGGAAGGAATGCTGGCCAACCTAGTGGAACAGAACATCTCAGTGCGCCGGCGC CAAGGGGTCAGCATTGGGCGGCTTCACAAGCAGCGGAAGCCTGACCGGCGGAAACGCTCTCGCCCCTACA AGGCCAAACGCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210327 representing NM_027088
Red=Cloning site Green=Tags(s) MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTS VIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSNVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKA HNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVI MERIGLATAGEPYHDIRFNLMAVVPDRRIKYETRLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQ LPEESKPASSKSPLGLEAGRTPVASECTQTDGAEEVAGSCPQTTTHSPPSKCKLVVKPPGSSLNGVPPNP APIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRAPQQYSEDEDDYEDEDEDVQNTNPAIRYKRK GTGKPGSLSNSSDGQLSVLQPNTINVLTEKLQESQKDLSVPLSIKTSSGAGSPAVAVPTHSQPSPTPSNE STDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGL LHLAEDGVLSPLALTEGGKGSSPSTRSSQGSQGSSGLEEKEVVEVTESRDKPGLNRSSEPLSGEKYSPKE LLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRR QGVSIGRLHKQRKPDRRKRSRPYKAKRQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027088 |
| ORF Size | 2184 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027088.2, NP_081364.1 |
| RefSeq Size | 3406 bp |
| RefSeq ORF | 2187 bp |
| Locus ID | 104416 |
| UniProt ID | Q99PU7 |
| Gene Summary | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202559 | Bap1 (untagged) - Mouse Brca1 associated protein 1 (Bap1), (10ug) |
CNY 2,000.00 |
|
| MR210327 | Bap1 (Myc-DDK-tagged) - Mouse Brca1 associated protein 1 (Bap1) |
CNY 5,344.00 |
|
| MR210327L3 | Lenti ORF clone of Bap1 (Myc-DDK-tagged) - Mouse Brca1 associated protein 1 (Bap1) |
CNY 8,270.00 |
|
| MR210327L4 | Lenti ORF clone of Bap1 (mGFP-tagged) - Mouse Brca1 associated protein 1 (Bap1) |
CNY 7,744.00 |
