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Plod3 (NM_011962) Mouse Tagged ORF Clone
CAT＃: MG210405
- TrueORF®
Plod3 (tGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3)
ORF Plasmid: DDK
"NM_011962" in other vectors (3)
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CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI414586; LH3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210405 representing NM_011962
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGCAGGCCCGGAACCCCGGCTTTTGCTCCTGCTCCTGCTGCTGCTGCCGCCGCTGCCCCCCG TAACTTCTGCCTCCGATCGACCCCGGGGCGCCAATGCTGTCAACCCAGACAAATTGCTGGTGATCACTGT GGCTACGGCAGAGACAGAGGGGTACCGGCGTTTTCTGCAGTCTGCGGAGTTCTTTAACTACACTGTACGG ACCCTGGGCCTGGGACAGGAGTGGCGAGGGGGTGATGTGGCTCGAACAGTTGGTGGAGGCCAGAAGGTCA GGTGGCTCAAGAAGGAAATGGAGAAATATGCAGATCAGAAAGACATGATCATCATGTTTGTGGACAGCTA CGACGTGATTCTGGCAAGCAGCCCGACAGAGCTGCTGAAGAAGTTTGTTCAGAGTGGCAGTCATCTGCTG TTCTCTGCTGAGAGCTTCTGCTGGCCTGAGTGGGGGCTGGCAGAGCAGTACCCTGAGGTGGGCATGGGGA AGCGCTTCCTCAACTCTGGTGGATTCATCGGCTTTGCTCCCACCATCCATCAGATTGTCCGCCAGTGGAA CTACAAAGACGACGATGATGATCAATTGTTCTACACTCAACTGTACCTGGACCCAGGGCTGCGGGAGAAA CTCAAACTGAGTCTGGACCATAAATCCCGGATCTTTCAGAACCTCAATGGAGCCTTAGATGAAGTGATCT TAAAGTTTGACCAGAATCGTGTGCGCATCCGGAATGTGGCCTACGACACACTTCCTGTTGTGGTCCATGG AAATGGTCCCACTAAGCTCCAGCTCAACTACCTGGGGAACTATGTCCCCAATGGCTGGACTCCCCAGGGA GGCTGTGGATTCTGCAACCAGACCCTGAGGACACTCCCGGGGGGGCAGCCTCCCCCCCGGGTGCTTCTGG CCGTGTTTGTGGAGCAGCCTACTCCCTTCCTGCCTCGGTTCCTGCAGCGCCTACTGCTCCTGGATTACCC CCCGGACAGGATCTCTCTCTTCCTTCACAACAGCGAGGTGTACCACGAGCCTCATATTGCAGATGCCTGG CCACAGCTCCAGGACCATTTTTCAGCTGTAAAGCTGGTGGGGCCAGAGGAGGCCCTGAGCGCAGGGGAGG CCAGGGACATGGCCATGGACAGCTGTCGGCAGAACCCTGAGTGTGAGTTCTACTTTAGCCTGGATGCTGA TGCCGTCCTTACGAACCCGGAGACCTTGCGTGTCTTGATTGAACAAAACAGGAAGGTAATAGCTCCCATG CTTTCCCGCCATGGCAAGCTCTGGTCCAACTTCTGGGGTGCCCTGAGCCCCAATGAGTACTACGCCCGCT CCGAAGACTACGTGGAACTGGTGCAGCGGAAGCGAGTGGGCGTGTGGAATGTACCCTACATATCTCAGGC ATATGTGATCCGCGGGGAGACCCTGAGGACTGAACTGCCCCAAAAGGAGGTATTCTCCAGCAGCGACACA GACCCAGATATGGCTTTCTGCAAGAGCGTCCGGGACAAGGGCATCTTCCTCCACCTCAGCAACCAGCACG AGTTTGGCCGGCTGCTGGCTACTTCTCGCTATGACACAGACCACCTACACCCAGACCTCTGGCAGATCTT TGACAACCCTGTGGACTGGAGAGAACAGTACATTCACGAGAATTACAGCCGGGCCCTGGATGGGGAAGGG CTAGTGGAACAGCCATGCCCAGATGTGTACTGGTTCCCACTGCTGACCGAGCAGATGTGTGATGAGCTGG TGGAGGAAATGGAACACTATGGCCAGTGGTCTGGAGGCCGGCACGAGGATTCCAGGTTGGCTGGAGGATA CGAGAATGTTCCCACCGTTGATATCCACATGAAACAGGTGGGATACGAGGACCAGTGGCTTCAGCTGCTG CGGACATATGTGGGGCCCATGACGGAGTACCTGTTCCCTGGCTACCACACCAAGACACGGGCAGTGATGA ACTTCGTGGTCCGCTACCGGCCAGATGAGCAGCCCTCGCTTCGGCCACACCATGACTCGTCCACCTTCAC TCTCAATGTCGCCCTCAACCACAAGGGTGTAGATTATGAGGGAGGCGGCTGCCGCTTCCTGCGTTACGAC TGCAGAATCTCCTCTCCGAGGAAAGGCTGGGCCCTCCTGCACCCTGGCCGCCTCACACACTACCATGAGG GGCTGCCCACCACCCGGGGTACTCGATACATCATGGTGTCCTTTGTTGACCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210405 representing NM_011962
Red=Cloning site Green=Tags(s) MAAAGPEPRLLLLLLLLLPPLPPVTSASDRPRGANAVNPDKLLVITVATAETEGYRRFLQSAEFFNYTVR TLGLGQEWRGGDVARTVGGGQKVRWLKKEMEKYADQKDMIIMFVDSYDVILASSPTELLKKFVQSGSHLL FSAESFCWPEWGLAEQYPEVGMGKRFLNSGGFIGFAPTIHQIVRQWNYKDDDDDQLFYTQLYLDPGLREK LKLSLDHKSRIFQNLNGALDEVILKFDQNRVRIRNVAYDTLPVVVHGNGPTKLQLNYLGNYVPNGWTPQG GCGFCNQTLRTLPGGQPPPRVLLAVFVEQPTPFLPRFLQRLLLLDYPPDRISLFLHNSEVYHEPHIADAW PQLQDHFSAVKLVGPEEALSAGEARDMAMDSCRQNPECEFYFSLDADAVLTNPETLRVLIEQNRKVIAPM LSRHGKLWSNFWGALSPNEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGETLRTELPQKEVFSSSDT DPDMAFCKSVRDKGIFLHLSNQHEFGRLLATSRYDTDHLHPDLWQIFDNPVDWREQYIHENYSRALDGEG LVEQPCPDVYWFPLLTEQMCDELVEEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLL RTYVGPMTEYLFPGYHTKTRAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGVDYEGGGCRFLRYD CRISSPRKGWALLHPGRLTHYHEGLPTTRGTRYIMVSFVDP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011962 |
| ORF Size | 2223 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011962.3 |
| RefSeq Size | 3186 bp |
| RefSeq ORF | 2226 bp |
| Locus ID | 26433 |
| UniProt ID | Q9R0E1 |
| Gene Summary | Multifunctional enzyme that catalyzes a series of post-translational modifications on Lys residues in procollagen (PubMed:16447251). Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:16447251). Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine (By similarity). Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:16447251, PubMed:16467571, PubMed:21220425). Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:25419660). Catalyzes hydroxylation and glycosylation of Lys residues in the ADIPOQ collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues (PubMed:23209641). Essential for normal biosynthesis and secretion of type IV collagens (PubMed:15377789, PubMed:16467571, PubMed:17873278). Essential for normal formation of basement membranes (PubMed:15377789, PubMed:16467571).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR210405 | Plod3 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) |
CNY 5,432.00 |
|
| MR210405L3 | Lenti ORF clone of Plod3 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) |
CNY 8,360.00 |
|
| MR210405L4 | Lenti ORF clone of Plod3 (mGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (Plod3) |
CNY 8,360.00 |
