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Rasgrp1 (NM_011246) Mouse Tagged ORF Clone
CAT＃: MG210704
- TrueORF®
Rasgrp1 (tGFP-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1)
ORF Plasmid: DDK
"NM_011246" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Rasgrp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210704 representing NM_011246
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCTTGCCATGGCTCCAGAGCTGGCCCCAAAG CAAGACTAGAGGCCAAATCAACCAACAGCCCTCTCCCTGCCCAGCCCAGCTTGGCCCAGATCACCCAGTT CCGAATGATGGTGTCCCTGGGACATTTGGCCAAAGGAGCCAGCCTGGATGATCTTATTGACAGCTGCATT CAATCTTTTGATGCGGATGGAAACCTGTGTCGGAATAACCAACTGTTACAAGTCATGCTAACCATGCACC GGATCATCATCTCCTCAGCCGAGCTGCTACAAAAAGTCATGAATCTATATAAGGATGCCCTGGAAAAGAA TTCTCCAGGAGTTTGCCTGAAGATCTGCTATTTTGTCAGGTATTGGATAACAGAATTCTGGATCATGTTC AAGATGGATGCCAGCTTGACCAGCACCATGGAAGAGTTCCAAGACCTGGTGAAAGCCAATGGTGAGGAGA CCCACTGCCACCTCATCGACACGACCCAAATTAATTCTCGAGACTGGTCCAGGAAACTGACTCAGAGGAT AAAATCGAATACTAGCAAGAAGCGCAAAGTGTCCCTGCTGTTTGACCATCTGGAACCTGAAGAACTGTCT GAACACCTCACCTACCTTGAGTTCAAGTCCTTCCGACGGATATCTTTCTCTGATTACCAAAATTACCTTG TAAATAGCTGCGTAAAAGAGAACCCCACCATGGAGAGGTCCATTGCCCTGTGCAATGGCATCTCCCAGTG GGTACAACTGATGGTTCTCAGCCGTCCCACCCCGCAGCTCCGGGCAGAGGTCTTCATCAAGTTCATCCAT GTGGCTCAGAAACTCCACCAGCTACAGAACTTCAACACGCTGATGGCTGTGATCGGGGGACTGTGTCACA GCTCCATCTCCAGGCTTAAGGAAACAAGTTCACATGTCCCACATGAGATCAATAAGGTTCTGGGCGAGAT GACTGAACTGCTGTCCTCCTGCAGAAACTATGACAACTACAGGCGAGCCTATGGGGAGTGCACCCACTTC AAAATCCCCATACTGGGTGTGCACCTCAAGGACCTCATATCCCTGTATGAGGCTATGCCCGACTATCTGG AAGATGGGAAGGTGAATGTCCAAAAGCTCCTGGCCCTTTACAATCATATCAATGAATTGGTCCAGCTGCA GGAGATGGCCCCACCATTGGATGCCAACAAGGACTTGGTGCATCTGCTGACGTTATCCCTGGATCTATAC TATACAGAAGATGAAATCTATGAGCTTTCCTATGCCCGGGAGCCGAGGAACCACAGAGCTCCGCCACTGA CACCTTCGAAGCCACCAGTTGTAGTAGACTGGGCCTCTGGAGTGTCTCCCAAACCTGACCCAAAGACCAT CAGCAAACACGTCCAAAGGATGGTGGATTCTGTCTTTAAGAACTATGATCTCGACCAGGATGGCTATATC TCTCAGGAGGAGTTTGAGAAGATCGCTGCAAGCTTTCCATTTTCCTTCTGTGTGATGGACAAAGATAGGG AAGGCCTCATCAGCAGAGACGAGATCACGGCCTACTTCATGAGGGCCAGCTCCATCTATTCCAAGCTGGG CCTGGGCTTTCCACACAACTTTCAAGAAACCACTTACCTGAAGCCCACCTTCTGTGACAACTGTGCTGGC TTTCTCTGGGGCGTGATCAAGCAAGGCTATCGCTGTAAAGACTGCGGGATGAACTGCCACAAACAGTGCA AAGACCTGGTGGTGTTCGAGTGCAAGAAGCGAATCAAGAGCCCAGCAATATCCACAGAGAACATCAGCTC TGTGGTACCAATGTCCACCCTTTGTCCACTGGGAACCAAAGATCTGCTCCATGCACCCGAAGAAGGATCT TTCATTTTCCAAAACGGAGAGATTGTGGACCACAGTGAGGAGAGCAAGGATAGGACCATCATGCTCCTCG GAGTGTCCTCCCAGAAAATTTCAGTTCGGCTGAAGAGGACTGTTGCCCACAAGAGCACCCAAACAGAATC GTTCCCGTGGGTTGGTGGCGAGACGACCCCTGGTCACTTTGTGCTGTCTTCTCCAAGGAAGTCGGCGCAG GGCGCTCTTTATGTGCACAGTCCAGCATCTCCATGCCCCAGCCCAGCACTGGTCCGAAAGCGGGCATTCG TCAAGTGGGAGAACAAAGAGTCCCTTATAAAACCAAAACCAGAACTTCACCTCCGGCTCCGGACCTACCA AGAACTGGAACAGGAAATAAATACCCTGAAAGCAGATAACGATGCTCTGAAGATCCAACTGAAGTACGCA CAGAAGAAAATAGAATCCCTGCAGCTTGGGAAAAGCAATCATGTCTTAGCCCAGATGGACCACGGTGATA GTGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210704 representing NM_011246
Red=Cloning site Green=Tags(s) MGTLGKAREAPRKPCHGSRAGPKARLEAKSTNSPLPAQPSLAQITQFRMMVSLGHLAKGASLDDLIDSCI QSFDADGNLCRNNQLLQVMLTMHRIIISSAELLQKVMNLYKDALEKNSPGVCLKICYFVRYWITEFWIMF KMDASLTSTMEEFQDLVKANGEETHCHLIDTTQINSRDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELS EHLTYLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIH VAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSCRNYDNYRRAYGECTHF KIPILGVHLKDLISLYEAMPDYLEDGKVNVQKLLALYNHINELVQLQEMAPPLDANKDLVHLLTLSLDLY YTEDEIYELSYAREPRNHRAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDLDQDGYI SQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAG FLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRIKSPAISTENISSVVPMSTLCPLGTKDLLHAPEEGS FIFQNGEIVDHSEESKDRTIMLLGVSSQKISVRLKRTVAHKSTQTESFPWVGGETTPGHFVLSSPRKSAQ GALYVHSPASPCPSPALVRKRAFVKWENKESLIKPKPELHLRLRTYQELEQEINTLKADNDALKIQLKYA QKKIESLQLGKSNHVLAQMDHGDSA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011246 |
| ORF Size | 2385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011246.3 |
| RefSeq Size | 5155 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 19419 |
| UniProt ID | Q9Z1S3 |
| Gene Summary | Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways (By similarity). Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses (PubMed:17190838). May also function in differentiation of other cell types. Proto-oncogene, which promotes T-cell lymphomagenesis when its expression is deregulated (PubMed:15829980, PubMed:17210708).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221698 | Rasgrp1 (untagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1), (10ug) |
CNY 10,260.00 |
|
| MR210704 | Rasgrp1 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1) |
CNY 5,800.00 |
|
| MR210704L3 | Lenti ORF clone of Rasgrp1 (Myc-DDK-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1) |
CNY 7,700.00 |
|
| MR210704L4 | Lenti ORF clone of Rasgrp1 (mGFP-tagged) - Mouse RAS guanyl releasing protein 1 (Rasgrp1) |
CNY 7,700.00 |
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