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Rasa1 (NM_145452) Mouse Tagged ORF Clone
CAT＃: MG210785
- TrueORF®
Rasa1 (tGFP-tagged) - Mouse RAS p21 protein activator 1 (Rasa1)
ORF Plasmid: DDK
"NM_145452" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Gap; Rasa; RasGAP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210785 representing NM_145452
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCGGCCGAGGCGGGCAGTGAGGAAGGCGGCCCTGCGACGGCGGGCCCCGGCGGCGGCGCGGCCA CGGGCTCCAGCGCCTACTCGGCAGCGTGCCGGGTCAAGTTACCCGCGGCCCTGCCGGTGGCAGCCGCCCC GTGTCCCGGGCTGGCGGACGCCGACTTGGTTGCGGCCCTCGGTGGCGGAGCCGCGTCGGGGTCCGGGTTC CTGGGGACCGGGCCGGTGGCCGGTGTCTTGGGGGGCGCCGCGCTGACCGGCAGTGCTGCTGCTGGCGTGG CTGGTGCTGCGGCCGCCGGACCTGCGGGAGACGTCGCTTTCACCAAGGGGACTCTGTCTTTGCCTGCTGA GACGCTCGGGCCGGGCGGCGGATTCCCTCCTCTGCCGCCGCCTCCTCAGCTGCCCCCTTTGGGCTCCGGT TTGGGGACCGTGGATGAAGGTGACTCCCTGGATGGACCAGAATACGAAGAGGAAGAGGTGGCCATACCGC TGACCGCACCTCCCACTAACCAGTGGTATCATGGAAAACTTGATAGAACTATAGCAGAAGAACGCCTCAG GCAGGCAGGGAAATCTGGAAGCTATCTTATAAGAGAGAGTGATCGGAGGCCGGGGTCCTTTGTACTTTCA TTCCTTAGTCAGACAAATGTTGTCAATCATTTTAGGATTATTGCTATGTGTGGTGATTACTACATTGGTG GAAGGCGTTTTTCTTCACTCTCAGACCTAATAGGTTATTACAGTCACGTTTCTTGTTTACTTAAAGGAGA AAAATTACTCTATCCAGTTGCACCACCAGAGCCAGTAGAAGATAGAAGGCGTGTACGAGCCATTCTGCCC TACACAAAAGTACCAGACACTGATGAAATAAGTTTTTTAAAAGGAGATATGTTCATTGTTCACAATGAGT TAGAAGATGGATGGATGTGGGTTACCAATCTAAGAACAGATGAGCAAGGCCTTATTGTTGAAGACCTAGT GGAAGAGGTGGGCCGAGAAGAAGATCCACACGAAGGCAAAATATGGTTCCACGGGAAAATTTCCAAACAA GAAGCTTATAATTTATTAATGACAGTTGGACAAGTCTGCAGCTTTCTTGTGAGGCCCTCAGATAATACTC CTGGAGATTATTCTCTGTATTTTCGGACCAATGAAAATATTCAGCGATTTAAAATTTGTCCAACACCTAA CAACCAGTTTATGATGGGAGGCCGCTATTATAACAGTATCGGGGACATCATAGACCATTATCGGAAAGAA CAGATTGTTGAAGGCTATTATCTTAAGGAACCTGTACCAATGCAGGATCAAGGGCAAGTACTGAACGACA CTGTGGATGGGAAGGAGATCTATAACACAATTCGTCGGAAGACAAAGGATGCTTTTTATAAAAATATTGT TAAAAAAGGTTATCTTCTGAAGAAGGGTAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAAGGC AGTGATGCTCAACTTATTTATTTTGAAAGTGAAAAACGAGCTACAAAACCAAAAGGGCTAATAGACCTTA GTGTGTGTTCTGTCTACGTTGTCCATGACAGTCTCTTCGGCAGGCCAAACTGTTTTCAGATAGTAGTTCA GCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATG AAAGGTCTGCAAGCATTTTGCAGTTTACGGAAAAGTAGCCCAGGGACATCTAATAAACGCCTTCGTCAGG TCAGCAGCCTTGTTTTACATATTGAAGAAGCCCATAAACTACCAGTAAAACACTTTACTAATCCATATTG TAACATCTACTTGAATAGCGTCCAAGTAGCAAAAACTCATGCAAGGGAGGGGCAAAACCCAGTATGGTCA GAGGAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATCACACTTAGTAATAAAACAA AGAAAAGCAAAGATCCAGATATCTTATTTATGCGTTGCCAGTTGAGCAGGTTACAGAAAGGACATGCAAC AGATGAATGGTTTCTACTAAGTTCCCATATACCACTAAAAGGTATTGAACCAGGATCTTTGCGTGTTCGA GCACGATACTCCATGGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAA AGGAACTTCATGTCGTCTATGCTTTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCT ACTAAAGATATTTCTTCATGAAAAGCTTGAATCATTGTTGTTATGCACACTAAATGACAGAGAGATAAGT ATGGAAGATGAAGCCACTACCCTATTTCGAGCTACAACACTTGCAAGTACCTTGATGGAGCAGTATATGA AAGCTACTGCCACACAGTTTGTTCATCATGCTTTGAAAGACTCCATTTTAAAGATTATGGAAAGCAAGCA ATCATGTGAGTTAAGTCCGTCAAAGTTAGAAAAAAATGAAGATGTGAACACTAATTTAGCACACCTATTG AGCATACTTTCAGAGCTTGTGGAGAAAATATTCATGGCTTCAGAAATACTTCCACCGACACTGAGATATA TCTATGGGTGTTTACAGAAATCAGTTCAGCATAAGTGGCCTACAAATAACACCATGAGAACAAGAGTCGT TAGTGGTTTTGTTTTTCTTCGCCTTATCTGCCCTGCTATCCTGAATCCACGGATGTTTAATATCATCTCA GATTCCCCATCTCCTATTGCTGCAAGAACACTGACATTAGTGGCTAAATCTGTGCAGAATTTAGCAAATC TTGTGGAATTTGGAGCTAAGGAGCCTTATATGGAAGGTGTCAATCCATTTATAAAAAGTAATAAACATCG CATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAACTTCCGGACACTACAGAGCATTCTAGAACT GACCTGTCTCGTGACTTAGCAGCATTGCATGAGATTTGTGTAGCTCATTCAGATGAACTGAGAACACTCA GCAATGAGCGTGGTGTACAGCAGCACGTACTGAAAAAGCTTCTGGCGATAACAGAACTGCTTCAACAAAA ACAAAACCAGTATACAAAAACCAATGACATCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210785 representing NM_145452
Red=Cloning site Green=Tags(s) MMAAEAGSEEGGPATAGPGGGAATGSSAYSAACRVKLPAALPVAAAPCPGLADADLVAALGGGAASGSGF LGTGPVAGVLGGAALTGSAAAGVAGAAAAGPAGDVAFTKGTLSLPAETLGPGGGFPPLPPPPQLPPLGSG LGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLS FLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILP YTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQ EAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKE QIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEG SDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWM KGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWS EEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVR ARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREIS MEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLL SILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIIS DSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRT DLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDIR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145452 |
| ORF Size | 3114 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145452.3, NP_663427.2 |
| RefSeq Size | 5101 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 218397 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203713 | Rasa1 (untagged) - Mouse RAS p21 protein activator 1 (Rasa1), (10ug) |
CNY 2,000.00 |
|
| MR210785 | Rasa1 (Myc-DDK-tagged) - Mouse RAS p21 protein activator 1 (Rasa1) |
CNY 7,232.00 |
|
| MR210785L3 | Lenti ORF clone of Rasa1 (Myc-DDK-tagged) - Mouse RAS p21 protein activator 1 (Rasa1) |
CNY 10,260.00 |
|
| MR210785L4 | Lenti ORF clone of Rasa1 (mGFP-tagged) - Mouse RAS p21 protein activator 1 (Rasa1) |
CNY 10,260.00 |
