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Enpp2 (NM_015744) Mouse Tagged ORF Clone
CAT＃: MG210991
- TrueORF®
Enpp2 (tGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2)
ORF Plasmid: DDK
"NM_015744" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AT; ATX; Auto; E-NPP 2; lysoPLD; N; Npps2; Pd; PD-; PD-Ialpha; Pdnp2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210991 representing NM_015744
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGACAAGGCTGTTTCGGGTCATACCAGGTAATATCCTTGTTCACTTTTGCCATCGGCGTCAATC TCTGCTTAGGATTCACAGCAAGTCGAATTAAGAGGGCCGAATGGGATGAAGGACCTCCCACAGTGTTATC TGACTCTCCATGGACCAACACATCTGGATCCTGCAAAGGTAGATGCTTTGAGCTTCAAGAGGTTGGACCT CCTGACTGTCGGTGTGACAACCTATGTAAGAGCTACAGCAGCTGCTGCCATGATTTTGATGAGCTCTGTT TGAAAACAGCTCGAGGCTGGGAGTGCACCAAAGACAGATGTGGGGAAGTACGAAATGAGGAAAATGCCTG TCACTGCTCAGAAGACTGCTTGTCCCGGGGAGACTGCTGTACCAACTATCAAGTGGTCTGCAAAGGAGAA TCACACTGGGTAGATGATGACTGTGAAGAAATAAGAGTCCCTGAATGCCCTGCAGGGTTTGTCCGCCCTC CGTTAATCATCTTCTCTGTGGATGGATTCCGTGCATCGTACATGAAGAAAGGCAGCAAGGTTATGCCCAA CATTGAGAAACTGCGGTCCTGTGGCACCCATGCTCCCTACATGAGGCCTGTGTACCCTACAAAAACCTTC CCTAATCTGTATACGCTGGCCACTGGTTTATATCCAGAATCCCATGGAATCGTTGGCAATTCAATGTATG ACCCTGTCTTTGATGCTACTTTCCATCTTCGAGGGCGAGAGAAGTTTAACCATAGATGGTGGGGAGGCCA ACCGCTATGGATTACAGCCACCAAGCAAGGGGTGAGAGCCGGGACATTCTTTTGGTCTGTGAGCATCCCT CACGAGCGGAGAATCCTAACTATCCTTCAGTGGCTTTCCCTGCCAGACAATGAGAGGCCTTCAGTTTATG CCTTCTACTCCGAGCAGCCTGATTTTTCTGGACACAAGTACGGCCCTTTTGGCCCTGAGATGACAAATCC TCTGAGGGAGATTGACAAGACCGTGGGGCAGTTAATGGACGGACTGAAACAACTCAAGCTGCACCGTTGT GTGAATGTTATCTTTGTTGGAGACCATGGAATGGAAGACGTGACATGTGACAGAACTGAGTTCTTGAGCA ACTATCTGACTAACGTGGATGATATTACTTTAGTACCTGGAACTCTAGGAAGAATTCGACCCAAGATTCC CAATAATCTTAAATATGACCCTAAAGCCATTATTGCTAACCTCACGTGTAAAAAACCAGATCAGCACTTT AAGCCTTACATGAAACAGCACCTTCCCAAACGTTTGCACTATGCCAACAATCGGAGAATCGAGGATCTCC ATTTATTGGTGGAACGCAGATGGCATGTTGCAAGGAAACCTTTGGACGTTTATAAGAAGCCGTCAGGAAA ATGTTTTTTCCAGGGTGACCACGGCTTTGATAACAAGGTCAATAGCATGCAGACTGTTTTTGTAGGTTAT GGCCCAACTTTTAAGTACAGGACTAAAGTGCCTCCATTTGAAAACATTGAACTTTATAATGTTATGTGCG ATCTCCTAGGCTTGAAGCCAGCTCCCAATAATGGAACACATGGAAGTTTGAATCACCTGCTACGCACAAA TACCTTTAGGCCAACCCTACCAGAGGAAGTCAGCAGACCCAATTACCCAGGGATTATGTACCTTCAGTCT GATTTTGACCTGGGCTGCACCTGTGATGATAAGGTAGAGCCAAAGAACAAATTGGAAGAACTAAATAAAC GCCTTCATACCAAAGGATCTACAGAAGAGAGACATCTCCTGTATGGACGACCTGCAGTGCTTTATCGGAC TAGCTATGATATCTTATACCATACGGACTTTGAAAGTGGTTACAGTGAAATATTCTTAATGCCTCTCTGG ACTTCTTATACCATTTCTAAGCAGGCTGAGGTCTCTAGCATCCCAGAGCACCTGACCAACTGTGTTCGCC CTGATGTCCGTGTATCTCCTGGATTCAGTCAGAACTGTTTAGCCTATAAAAATGATAAACAGATGTCCTA TGGATTCCTTTTTCCTCCCTATCTGAGCTCTTCCCCAGAAGCGAAATATGATGCATTCCTTGTAACCAAC ATGGTTCCAATGTACCCTGCCTTCAAACGTGTTTGGACTTATTTCCAAAGGGTCTTGGTGAAGAAATATG CGTCAGAAAGGAATGGGGTCAACGTAATAAGTGGACCGATCTTTGACTACAATTACGATGGCTTACGTGA CATTGAGGATGAAATTAAACAGTATGTGGAAGGCAGCTCTATTCCTGTCCCTACCCACTACTACAGCATC ATCACCAGCTGCCTGGACTTCACTCAGCCTGCAGACAAGTGTGATGGTCCTCTCTCTGTGTCTTCTTTCA TCCTTCCTCACCGACCTGACAATGATGAGAGCTGTAATAGTTCCGAGGATGAGTCGAAGTGGGTAGAGGA ACTCATGAAGATGCACACAGCTCGGGTGAGGGACATCGAGCATCTCACCGGTCTGGATTTCTACCGGAAG ACTAGCCGTAGCTATTCGGAAATTCTGACCCTCAAGACATACCTGCATACATATGAGAGCGAGATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210991 representing NM_015744
Red=Cloning site Green=Tags(s) MARQGCFGSYQVISLFTFAIGVNLCLGFTASRIKRAEWDEGPPTVLSDSPWTNTSGSCKGRCFELQEVGP PDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGE SHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTF PNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIP HERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRC VNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHF KPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGY GPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQS DFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLW TSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTN MVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYDGLRDIEDEIKQYVEGSSIPVPTHYYSI ITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRK TSRSYSEILTLKTYLHTYESEI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015744 |
| ORF Size | 2586 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015744.1, NP_056559.1 |
| RefSeq Size | 3242 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 18606 |
| UniProt ID | Q9R1E6 |
| Gene Summary | This gene encodes a member of the phosphodiesterase and nucleotide pyrophosphatase family of bifunctional enzymes that hydrolize phosphodiester bonds of various nucleotides. The encoded protein undergoes proteolytic processing to generate a mature protein with lysophospholipase D activity, catalyzing the cleavage of the choline group from lysophosphatidylcholine to produce lysophosphatidic acid. This gene is expressed in numerous tissues and participates in neural development, obesity, inflammation and oncogenesis. A complete lack of the encoded protein in mice results in aberrant vascular and neuronal development leading to embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204624 | Enpp2 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2, (10ug) |
CNY 2,000.00 |
|
| MR210991 | Enpp2 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2 |
CNY 6,320.00 |
|
| MR210991L3 | Lenti ORF clone of Enpp2 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2 |
CNY 9,030.00 |
|
| MR210991L4 | Lenti ORF clone of Enpp2 (mGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2 |
CNY 9,030.00 |
