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Ano1 (NM_178642) Mouse Tagged ORF Clone
CAT＃: MG211299
- TrueORF®
Ano1 (tGFP-tagged) - Mouse transmembrane protein 16A (Tmem16a)
ORF Plasmid: DDK
"NM_178642" in other vectors (4)
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CNY 9,392.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Tmem16a |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211299 representing NM_178642
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGACGCGCAGGACAGCGACATCGGCCTGGAGGGCTTGACCCCAGAGGGAACCTCTGCGGACCGAG AGTGCCAGCGGGGACCGGAAACAATCGCGCACGAGGCACAGGACGCAGGAACCCCCAACTCAGACGCCAC CGGGGTTGTGGATGGGGAGCGCGAGGCCACCATGAGGGTCCCCGAGAAGTACTCGACGCTCCCGGCGGAG GACCGCAGCGTCCACATCGTGAACATCTGCGCCATCGAGGACCTGGGCTACCTGCCGTCCGAGGGCACGT TGCTGAACTCTCTGTCCGTGGACCCCGACGCCGAATGCAAGTATGGACTATACTTCAGGGATGGCAAACG GAAGGTGGACTACATCTTGGTATACCATCACAAGAGAGCCTCAGGGAGCAGGACTCTGGCCAGGAGGGGA CTACAAAATGACATGGTCCTGGGGACCCGCAGCGTCAGGCAGGACCAGCCCCTTCCCGGGAAGGGGAGCC CTGTGGATGCAGGCTCACCGGAAGTCCCCATGGATTACCATGAAGATGACAAACGCTTCAGACGGGAGGA ATATGAGGGCAACCTGTTGGAGGCAGGCCTGGAGTTGGAGAATGACGAGGATACCAAAATCCATGGTGTC GGGTTTGTGAAGATCCATGCGCCCTGGCATGTGCTCTGTAGGGAAGCTGAGTTTTTGAAACTAAAGATGC CCACAAAGAAGGTGTACCACATCAGTGAGACGCGAGGCCTCCTGAAAACCATCAACTCGGTTCTGCAGAA GATCACAGACCCCATCCAGCCCAAGGTGGCTGAGCACAGGCCACAGACCACAAAGAGGCTCTCCTATCCC TTCTCCCGGGAGAAGCAACACCTATTCGACCTGACTGACAGGGACTCTTTTTTCGACAGCAAAACCCGGA GCACAATAGTCTATGAAATCCTGAAGAGAACAACGTGCACCAAGGCCAAGTACAGCATGGGTATCACCAG CCTCCTGGCCAATGGCGTATACTCAGCTGCATACCCTCTGCACGATGGGGACTATGAGGGTGACAACGTT GAGTTCAACGACAGGAAACTCCTGTATGAGGAATGGGCAAGTTACGGAGTCTTCTACAAATACCAGCCCA TTGACCTGGTCAGGAAATACTTTGGTGAGAAGGTTGGCCTGTACTTTGCCTGGCTTGGAGCCTACACCCA GATGCTCATCCCTGCCTCGATCGTGGGTGTCATTGTCTTTCTCTATGGATGTGCCACTGTGGACGAAAAC ATCCCCAGTATGGAGATGTGTGACCAGAGATACAACATCACCATGTGTCCTCTGTGTGACAAGACCTGCA GCTACTGGAAGATGAGCTCAGCCTGTGCCACAGCCCGTGCCAGTCACCTTTTTGATAACCCTGCCACCGT CTTCTTCTCTGTGTTTATGGCCCTCTGGGCTGCCACTTTCATGGAGCACTGGAAACGGAAGCAGATGAGG CTCAACTACCGATGGGACCTCACAGGCTTCGAGGAGGAGGAGGAAGCTGTCAAGGATCATCCCAGAGCAG AGTATGAAGCCAGAGTCTTAGAGAAGTCACTGAGAAAAGAATCCAGAAACAAAGAGACCGACAAGGTGAA GCTGACCTGGAGGGACCGATTCCCAGCCTATTTCACCAATCTTGTCTCCATCATCTTCATGATCGCAGTG ACATTTGCAATCGTCCTCGGAGTTATCATCTATAGAATCTCCACAGCTGCAGCCTTGGCCATGAACTCCT CCCCGTCTGTGCGGTCCAACATCCGGGTTACAGTCACGGCCACCGCTGTTATCATCAACCTCGTGGTCAT CATTCTGCTGGATGAAGTTTACGGCTGCATTGCCAGGTGGCTCACCAAGATTGAGGTCCCAAAGACAGAG AAGAGCTTTGAGGAGAGGCTAACCTTCAAGGCCTTCCTGCTCAAGTTTGTGAACTCTTACACTCCCATCT TCTATGTCGCCTTCTTCAAAGGCCGGTTTGTTGGTCGGCCCGGTGACTACGTGTACATCTTCCGCTCTTT CCGGATGGAGGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCATTATGCTG GGCAAGCAGCTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATCCGCTACC TGAAGCTGCGCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATGAGGTGGA CTTCAACCTCGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGGCTTTGTC ACCCTGTTTGTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAGATCCGCC TGGATGCCAAAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCGGCATCTG GTATAACATCCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTTCACGTCT GACTTCATCCCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTCGTCAATC ACACGCTCTCTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGGACCTGGG CTATGAGGTTCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTATGACATC TCCAAAGACTTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTGGTGATGT TCATGAGTGACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCCACAAAGA GAAGGTTCTCATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACACATGGATG GAGAAGGAGAAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCAGGCGACG GCAGCCCAGTCCCCAGCTACGAGTACCATGGGGACGCGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211299 representing NM_178642
Red=Cloning site Green=Tags(s) MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSDATGVVDGEREATMRVPEKYSTLPAE DRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARRG LQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHGV GFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSYP FSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDNV EFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDEN IPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQMR LNYRWDLTGFEEEEEAVKDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAV TFAIVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTE KSFEERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIML GKQLIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFV TLFVASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTS DFIPRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDI SKDFWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWM EKEKPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178642 |
| ORF Size | 3051 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178642.5, NP_848757.4 |
| RefSeq Size | 4614 bp |
| RefSeq ORF | 3054 bp |
| Locus ID | 101772 |
| UniProt ID | Q8BHY3 |
| Gene Summary | Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205263 | Ano1 (untagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1), (10ug) |
CNY 2,000.00 |
|
| MR211299 | Ano1 (Myc-DDK-tagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1) |
CNY 10,170.00 |
|
| MR211299L3 | Lenti ORF clone of Ano1 (Myc-DDK-tagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1) |
CNY 12,070.00 |
|
| MR211299L4 | Lenti ORF clone of Ano1 (mGFP-tagged) - Mouse anoctamin 1, calcium activated chloride channel (Ano1) |
CNY 12,070.00 |
