Dhx34 (BC080856) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Ddx34, mKIAA0134
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211703 representing BC080856 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCTCCCAGGACAAGGGAGGGGAGGGGTCACCGAGACCGAGACCACCATCGAGCTCCCAGGGAGG AAGAGGCCCCGGAGAAATGGGACTGGAATTGTCCAGAGACTCGTTGTCTCTTGGAAGATGTATTCTTCCG CGATGAGGATTATATCCGTCGTGGTTCAGAGGAGTGTCAGAAGTTCTGGGCTTTCTTTGAACGCCTGCAG AGATTCCAGCACCTCAAGACCTCCCAGAAGAAGGAGAAGGACCCTGGCATGCCCAAACATGGCATCGCTG CTTTAGCTGACCTACCTCTCACTTACGACCCACGCTACCGCATCAACCTCTCCATTCTCAGCCCAGACAC TCGGGGTCGTCATGGGCCCGGCAGGGGGCTGCCGCCCGAGAGAGTGTCCGAGTTCCGCCGTGCATTGCTT CACTACCTAGACTTCCAGCAGAAGCAGGCTTTTGGGAGGCTCGTCAAACTGCAGCGTGAGCGAGCGGCAC TTCCCATCGCCCAGTATGGGAACCGCATCCTGCAGACTCTCAAGGAACACCAGGTGGTGGTGGTGGCCGG GGACACAGGCTGTGGCAAGTCTACTCAGGTGCCCCAGTACTTGCTGGCCGCCGGCTTCAGTCACGTGGCA TGCACTCAGCCCCGGAGAATCGCCTGCATCTCACTGGCCAAGCGCGTTGGCTTCGAGAGCCTCAGTCAGT ATGGCTCCCAGGTTGGCTACCAGATCCGCTTTGAAAGCACTCGGTCGGCAGCCACCAAGATCGTGTTCCT GACGGTGGGGCTGCTCCTACGCCAGATCCAGCGTGAGCCCAGCCTGCCCCAGTACCAGGTCCTGATCGTG GACGAGGTCCACGAGCGACACCTGCACAATGACTTCCTGCTGGGTGTCCTGCAGCGCCTGCTGCCCCAGC GGCCTGACCTCAAGGTCATCCTCATGTCGGCCACCATCAACATCTCTCTCTTCTCCAGCTACTTCAGCCA TGCTCCGGTGGTGCAGGTGCCTGGGAGGCTCTTCCCCATCACGGTAGTCTACCAGCCGCAGGAGGCAGAC CAGACAGCATCCAAGTCAGAGAAGCTGGACCCACGGCCCTTCCTGAGGGTGCTGGAGGCCATCAACAATA AGTATCCCCCCGAGGAGCGGGGGGACCTTCTTGTCTTCCTCAGTGGCATGGCGGAGATCACCACGGTGCT GGACGCAGCCCAGGCTTATGCCAGCCTCACCCAGCGCTGGGTGGTGCTGCCGCTGCACAGCGCCCTCTCT GTGTCTGACCAGGACAAGGTGTTCGATGTGGCTCCAGCTGGAGTCCGGAAGTGCATCCTGTCCACCAACA TTGCCGAGACCTCAGTCACGATCGATGGCATCCGCTTCGTGGTGGATTCTGGGAAGGTGAAGGAGATGAG CTACGACCCACAGGCCAAACTGCAGCGGCTGCAGGAATTCTGGATCAGCCAGGCCAGTGCTGAGCAGCGC AAGGGCCGAGCGGGCCGCACGGGCCCCGGCGTCTGCTACCGTCTCTATGCCGAGTCTGACTATGATGCCT TTGCGCCGTACCCAGTCCCGGAAATCCGCAGGGTGGCCCTGGATGCACTGGTGCTTCAGATGAAGAGTAT GAGTGTGGGGGACCCACGCACCTTCCCCTTCATCGAGCCCCCTCCACCTGCCAGCGTGGAAACCGCTATC CTCTATCTCCAAGAGCAGGGGGCCCTGGACAGCTCGGAGGCCCTCACCCCCATCGGGTCCCTGCTGGCCC AGCTGCCTGTAGATGTTGTAATTGGGAAGATGCTGATCCTCGGCTCCATGTTCAGCCTGGCAGAGCCTGT GCTCACCATTGCTGCTGCCCTCAGCGTCCAGTCACCATTCACCCGCAGTGCCCAGAGCAACCTGGACTGT GCCACTGCCCGGAGACCGCTGGAAAGTGACCAGGGTGACCCCTTCACACTCTTCAATGTCTTCAATGCCT GGGTGCAGGTGAAGTCTGAGCGGAGCGGAAACTCTCGAAAGTGGTGCCGCCGCCGGGGTGTGGAGGAGCA CCGGCTGTATGAGATGGCCAACCTGCGGCGCCAGTTCAAGGAGCTGTTGGAGGATCATGGATTGCTGAGT GGGGCCCAGGTAGTGGCCCCAGGGGACAGCTACAGTCGGCTGCAGCAGCGCCGAGAGCGCAGGGCCCTGC ACCAGCTGAAGCGACAGCATGAGGAGGGTGGGGGACGCAGGCGCAAGGTGCTGAGGCTGCAGGAGGATGG CTGCTCCAGCGATGAAGAGGACCGAGAGGGCTCTACCTCCCAGAGGGCTGACAGCGTTGACATCCAGGAT GTGAAATTCAAGCTTCGGCACAACCTGGAGCAGCTGCAGGCAGCTGCCAGCTCAGCCCAGGACCTGACCC GAGACCAACTGGCCCTGCTCAAGCTGGTGCTCGGGCGGGGGCTGTACCCACAGCTGGCTGTGCCCGATGC GTTCAACAGTGGCCGCAAGGACTCAGACCAGATTTTCCACACGCAGGCCAAGCAGGGCACCGTGCTGCAC CCCACCTGTGTCTTTGCCAACAGCCCTGAGGTATTACACACACAGGGGCAGGAGGCCTCAGGCCAAGAAG GGAGCCAAGATGGCAGGGACCAAATGAGCTGCAAGCATCAGCTGCTGGCCTTCGTCTCCCTACTGGAGAC CAACAAGCCATACCTGGTGAACTGCGTCAGAATCCCGGCCCTCCAGTCCCTCCTGCTGTTCAGCCGCTCC ATTGACACCAACGGTGACTGCTCCCGCCTGGTGGCTGACGGTTGGCTAGAGCTGCAGCTCGCAGATAGCG AGAGTGCCGTCCGGCTCCTGGCAACATCCCTGCGGCTCCGTGTCCACTGGGAAAGTGCCCTGGACCGGCA GCTGGCCCGCCAGGCCCAGCGACGGAAGCTAGAGCAGGAGGAAGACATGGGGTCTCCAGCAGTCAGCCCC CAGGAGGTGGCTGCTCTGAGCAGGGAGCTGCTGCAGTTCATGGTGGCCAAGGTTCCCTACCGCCTCCGAA GGCTCACTGGTCTAGAAGCCCAGAACCTCTACGTGGGACCCCAGACCATCACGACCGCCCCTAGTCTGCC CGGCCTCTTTGGAAATTCTACCCTGTCTCCACACCCCACCAAGGGGGGCTACGCTGTCTCTGATTATCTC ACCTACAACTGCCTCACGAGTGACACTGACCTGTACAGTGACTGTCTGCGTTCCTTCTGGACCTGCCCAC ACTGTGGCCTGCACATGCCCTTCACACCCTTGGAGCGCATTGCTCATGAGAACACCTGCCCTGAGGCGCC AGGCGATGATCCAGGGTCTGAGGAGGCTGCTCCTGCACCACCACAGAAGACATCAGCCCTGCAGAGGCCC TACCACTGCCAGGTGTGCGGGCAGGACTTCCTCTTTACCCCAACTGAGGTACTAAGACATCGAAGGCAGC ATGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211703 representing BC080856 Red=Cloning site Green=Tags(s) MPPPRTREGRGHRDRDHHRAPREEEAPEKWDWNCPETRCLLEDVFFRDEDYIRRGSEECQKFWAFFERLQ RFQHLKTSQKKEKDPGMPKHGIAALADLPLTYDPRYRINLSILSPDTRGRHGPGRGLPPERVSEFRRALL HYLDFQQKQAFGRLVKLQRERAALPIAQYGNRILQTLKEHQVVVVAGDTGCGKSTQVPQYLLAAGFSHVA CTQPRRIACISLAKRVGFESLSQYGSQVGYQIRFESTRSAATKIVFLTVGLLLRQIQREPSLPQYQVLIV DEVHERHLHNDFLLGVLQRLLPQRPDLKVILMSATINISLFSSYFSHAPVVQVPGRLFPITVVYQPQEAD QTASKSEKLDPRPFLRVLEAINNKYPPEERGDLLVFLSGMAEITTVLDAAQAYASLTQRWVVLPLHSALS VSDQDKVFDVAPAGVRKCILSTNIAETSVTIDGIRFVVDSGKVKEMSYDPQAKLQRLQEFWISQASAEQR KGRAGRTGPGVCYRLYAESDYDAFAPYPVPEIRRVALDALVLQMKSMSVGDPRTFPFIEPPPPASVETAI LYLQEQGALDSSEALTPIGSLLAQLPVDVVIGKMLILGSMFSLAEPVLTIAAALSVQSPFTRSAQSNLDC ATARRPLESDQGDPFTLFNVFNAWVQVKSERSGNSRKWCRRRGVEEHRLYEMANLRRQFKELLEDHGLLS GAQVVAPGDSYSRLQQRRERRALHQLKRQHEEGGGRRRKVLRLQEDGCSSDEEDREGSTSQRADSVDIQD VKFKLRHNLEQLQAAASSAQDLTRDQLALLKLVLGRGLYPQLAVPDAFNSGRKDSDQIFHTQAKQGTVLH PTCVFANSPEVLHTQGQEASGQEGSQDGRDQMSCKHQLLAFVSLLETNKPYLVNCVRIPALQSLLLFSRS IDTNGDCSRLVADGWLELQLADSESAVRLLATSLRLRVHWESALDRQLARQAQRRKLEQEEDMGSPAVSP QEVAALSRELLQFMVAKVPYRLRRLTGLEAQNLYVGPQTITTAPSLPGLFGNSTLSPHPTKGGYAVSDYL TYNCLTSDTDLYSDCLRSFWTCPHCGLHMPFTPLERIAHENTCPEAPGDDPGSEEAAPAPPQKTSALQRP YHCQVCGQDFLFTPTEVLRHRRQHV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC080856
ORF Size	3435 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC080856, AAH80856
RefSeq Size	4097 bp
RefSeq ORF	3437 bp
Locus ID	71723
Gene Summary	Probable ATP-binding RNA helicase.[UniProtKB/Swiss-Prot Function]

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