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Nedd9 (NM_017464) Mouse Tagged ORF Clone
CAT＃: MG218896
- TrueORF®
Nedd9 (tGFP-tagged) - Mouse neural precursor cell expressed developmentally down-regulated gene 9 (Nedd9) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_017464" in other vectors (5)
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CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cas-L; CasL; HEF1; MEF1; Nedd-9; p105 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218896 representing NM_017464
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTACAAGAATCTTATGGCAAGGGCCTTGTATGACAACGTCCCTGAGTGTGCTGAGGAGCTGGCCT TCCGCAAGGGAGACATCTTAACTGTCATAGAGCAGAACACAGGAGGGCTTGAGGGATGGTGGCTGTGTTC CCTCCACGGTCGCCAAGGCATTGTCCCAGGGAACCGGGTGAAGCTTCTGATTGGTCCAGTGCAAGAGACC CCCGGTCATGAGCAGCCTACTCCTGGACCTATGCATCAGACCTTTGGCCAACAGAAACTCTATCAAGTGC CAAATTCCCAGGCAGCATCTCGGGATACCATCTACCAAGTGCCACCCTCCTACCAGAATCAGGGAATTTA CCAAGTACCCACTGGCCATGGCACTCCAGAACAAGATGTATATCAAGTACCACCATCAGTTCAGAGGAAC ATTGGCGGCACTAATGGACCCCTTCTAAGCAAAAAGGTGATCACCCCAGTGAGGACGGGCCATGGCTATG TGTACGAGTACCCATCCAGATACCAAAAGGATGTCTACGATGTCCCTCCTTCCCACAGCACTCAAGGGGT ATATGACATCCCTCCTTCCTCAGTAAAAGGCCCTGTGTTTTCAGTTCCAGTGGGAGAGATAAAACCTCAA GGGGTATATGACATTCCCCCCACCCAAGGGGTCTATGCCATTCCACCATCGGCTTGCCGAGATGAGGCAG GGCTCAGGGAAAAGGAATATGATTTCCCTCCTCCAATGAAGCAAGATGGAAAACCAGACACCAGACCTGA GGGGGTTTATGACATCCCTCCAACCAGCACCAAGACAGCAGGCAAGGACCTTCACATCAAATTCCCCTGT GATGCTCCAGGAGGTGTCGAACCAATGGCACGAAGACACCAGAGCTTTTCCCTGCACCATGCACCCTCTC AGCTGGGACAGTCTGGGGACACTCAGAGTGATGCCTATGATGTCCCCCGGGGAGTTCAGTTTCTGGAGGT ACCAACAGAAACCAGTGAAAAGGCAAATCCGGAGGAAAGAGACGGTGTCTACGATGTCCCTCTGCACAAC CCAGCAGATGCCAAAGGCTCTCGGGACGTGGTAGATGGGATCAACAGACTGTCTTTCTCCAGCACTGGCA GTACCAGGAGTAACATGTCCACCTCTTCCACCTCCTCAAAGGAGTCTTCACTGTCAGCCTCCCCGTCTCA AGACAAAAGGCTCCGACTGGACCCAGACACAGCCATAGAGAAGCTCTATCGGCTCCAGCAGACCCTGGAG ATGGGTGTGTGCAGCCTCATGTCACTGGTCACCACAGACTGGAGGTGCTACGGATACATGGAAAGGCACA TCAATGAGATCCGCACCGCGGTGGACAAAGTAGAGCTGTTCTTACGAGAATACCTCCATTTTGCCAAGGG AGCTTTAGCCAATGCCTCCTGCCTCCCAGAACTGGTCCTCCACAACAAAATGAAGCGGGAACTCCAAAGA GTAGAAGATTCCCACCAGATTCTAAGCCAAACCAGCCATGACTTGAATGAATGCAGCTGGTCCCTGAATA TTTTAGCTATCAATAAGCCCCAAAATAAGTGTGATGACCTAGACCGGTTTGTGATGGTCGCCAAGACAGT GCCAGACGACGCCAAACAACTGACCACCACCATCAGCACCTACGCGGAGACCCTCTTTAGAGCAGATCCT GCCAATTCCCATCTGAAGAATGGGCCCAACAGCATCATGAACTCAAGCGAGTACACACATCCGGGCTCCC AGATGCAGCCACTGCATCCTGGTGACTACAAAGCCCAGGTCCACAGTAAGCCGTTGCCTCCTAGTCTAAG CAAGGACCAGCCACCAGACTGCGGTAGCAGTGACGGTTCTGAGCGGAGTTGGATGGATGATTATGATTAT GTTCACCTACAGGGCAAGGAGGAGTTTGAGCGACAGCAGAAGGAGCTCTTGGAAAAGGAGAACATCATGA AGCAGAGTAAGGCGCAGCTGGAGCATCACCAGCTGAGTCAGTTCCAGCTGTTGGAACAAGAGATCACCAA GCCTGTGGAGAATGACATCTCTAAATGGAAGCCCTCTCAGAGCCTCCCAACCACCAACAACAGTGTGGGT GCTCAGGATAGGCAGTTGCTTTGCTTCTACTATGACCAGTGCGAGACCCATTTCATTTCCCTACTCAACG CCATCGACGCCCTCTTCAGCTGCGTCAGCTCAGCCCAACCCCCACGGATCTTTGTGGCGCACAGCAAGTT TGTCATTCTTAGTGCGCACAAACTGGTGTTCATTGGAGACACTCTGACAAGGCAGGTGGCTGCCCAGGAC ATTCGCAACAAAGTCAGGAACTCCAGCAACCAGCTCTGCGAACAGCTCAAGACGATAGTGATGGCGACCA AAATGGCCGCCCTCCACTACCCCAGTACCACCGCCTTGCAGGAAATGGTGCACCAGGTGACAGACCTGTC CAGAAATGCTCAGCTGTTTAAGCGTTCCTTGCTGGAGATGGCCACCTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218896 representing NM_017464
Red=Cloning site Green=Tags(s) MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRVKLLIGPVQET PGHEQPTPGPMHQTFGQQKLYQVPNSQAASRDTIYQVPPSYQNQGIYQVPTGHGTPEQDVYQVPPSVQRN IGGTNGPLLSKKVITPVRTGHGYVYEYPSRYQKDVYDVPPSHSTQGVYDIPPSSVKGPVFSVPVGEIKPQ GVYDIPPTQGVYAIPPSACRDEAGLREKEYDFPPPMKQDGKPDTRPEGVYDIPPTSTKTAGKDLHIKFPC DAPGGVEPMARRHQSFSLHHAPSQLGQSGDTQSDAYDVPRGVQFLEVPTETSEKANPEERDGVYDVPLHN PADAKGSRDVVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPSQDKRLRLDPDTAIEKLYRLQQTLE MGVCSLMSLVTTDWRCYGYMERHINEIRTAVDKVELFLREYLHFAKGALANASCLPELVLHNKMKRELQR VEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTISTYAETLFRADP ANSHLKNGPNSIMNSSEYTHPGSQMQPLHPGDYKAQVHSKPLPPSLSKDQPPDCGSSDGSERSWMDDYDY VHLQGKEEFERQQKELLEKENIMKQSKAQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNNSVG AQDRQLLCFYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVAAQD IRNKVRNSSNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017464 |
| ORF Size | 2499 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017464.5, NP_059492.3 |
| RefSeq Size | 4384 bp |
| RefSeq ORF | 2502 bp |
| Locus ID | 18003 |
| UniProt ID | O35177 |
| Gene Summary | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201926 | Nedd9 (untagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2, (10ug) |
CNY 2,000.00 |
|
| MG210874 | Nedd9 (tGFP-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9) |
CNY 9,120.00 |
|
| MR218896 | Nedd9 (Myc-DDK-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2 |
CNY 8,270.00 |
|
| MR218896L3 | Lenti ORF clone of Nedd9 (Myc-DDK-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2 |
CNY 10,170.00 |
|
| MR218896L4 | Lenti ORF clone of Nedd9 (mGFP-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9), transcript variant 2 |
CNY 10,170.00 |
