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Clcn1 (NM_013491) Mouse Tagged ORF Clone
CAT＃: MG220414
- TrueORF®
Clcn1 (tGFP-tagged) - Mouse chloride channel 1 (Clcn1), (10ug)
ORF Plasmid: DDK
"NM_013491" in other vectors (4)
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CNY 10,830.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | adr; Clc-1; Clc1; mto; myotonia; nmf355; SMCC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220414 representing NM_013491
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGTCCCAGTCCCAGCGGCATGGAGGGGAACAGAGCTGGTGGGGCAGTGCCCCCCAGTACCAGT ACATGCCCTTTGAACATTGTACCAGCTACGGACTGCCCTCAGAGAATGGGGGCCTTCAGCACCGGCCCCG AAAGGACATGGGTCCCAGGCACAATGCCCACCCAACACAGATATATGGCCATCAAAAAGAACAATATTCA TATAAGGCACAGGACGGGGGAATGCCCAAGAAGATGGGCTCCAGTTCTACCATGGACAGCTTGGATGAGG ACCACTATTCTAAATGTCAAGACTGTGTCCATCGCCTGGGACGTGTGCTGAGAAGGAAGCTGGGGGAAGA TTGGATCTTTCTTGTGCTCCTGGGCCTCCTGATGGCTCTAGTCAGCTGGTGCATGGACTATGTTAGTGCC AAGAGCCTTCAGGCCTACAAGTGGACCTATGCCCAGATGAAGCCTAGCCTTCCTCTGCAGTACCTGGCCT GGGTCACCTTCCCACTTATCCTGATCCTCTTCAGCGCCCTCTTTTGCCAACTCATCTCTCCCCAGGCTGT GGGCTCTGGAATCCCTGAGATGAAAACAATTCTTCGTGGTGTTGTCTTGAAGGAATACCTCACACTCAAG GCCTTTGTAGCCAAGGTGGTAGCTTTGACAGCTGGACTGGGCAGTGGCATCCCTGTGGGGAAAGAGGGTC CCTTTGTTCACATCGCCAGCATCTGTGCTGCTGTCCTCAGCAAGTTTATGTCCATGTTCTCTGGTGTCTA TGAGCAGCCATACTATTACACTGACATCCTGACAGTGGGCTGTGCCGTGGGGGTCGGTTGCTGTTTTGGA ACACCACTTGGAGGAGTGCTATTTAGCATCGAGGTCACCTCTACCTACTTTGCTGTTCGGAATTACTGGC GAGGATTCTTTGCAGCCACATTCAGTGCCTTTGTGTTCCGTGTCCTGGCCGTTTGGAACAAGGATGCTGT CACCATCACTGCTCTGTTCAGAACGAATTTCCGAATGGATTTCCCCTTTGACCTGAAGGAACTCCCAGCT TTTGCTGTCATTGGGATTTGCTGCGGGTTCTTGGGAGCTGTTTTCGTTTATCTGCATCGCCAAGTCATGC TCGGTGTCCGAAAGCACAAGTGTCTCAGCCAATTTCTTGCTAAGCACCGCCTGCTATATCCTGGAATTGT TACCTTTGTCATCGCCTCGCTCACATTTCCACCAGGAATGGGTCAATTCATGGCTGGAGAGCTGATGCCC CGTGAAGCTATCAGTACCCTCTTTGACAACAACACATGGGTAAAGCACATAGGTGACCCCCAAAGCTTGG GCCAGTCAGCTGTGTGGCTTCACCCCCAGGTCAACGTGATCATCATCATCCTTCTCTTCTTCGTTATGAA GTTTTGGATGTCCATTGTAGCCACCACTATGCCCATACCCTGTGGAGGCTTCATGCCTGTTTTTGTGCTA GGAGCTGCATTTGGAAGGCTGGTAGGAGAGATCATGGCCATGCTATTCCCTGAGGGTATCTTATTTGATG ATATCATCTATAAGATCTTACCTGGGGGCTATGCAGTAATTGGAGCAGCAGCTTTGACAGGGGCTGTCTC CCACACAGTCTCCACAGCCGTCATTTGCTTCGAATTAACCGGTCAGATTGCTCACATCCTGCCCATGATG GTGGCTGTTATCTTGGCCAACATGGTGGCTCAGAGTCTGCAGCCCTCCCTCTATGACAGCATCATCCAGG TCAAGAAGCTTCCCTATCTGCCAGACCTTGGTTGGAACCAGCTCAGCAAATTTACAATTTTTGTTGAGGA CATCATGGTACGTGATGTGAAGTTCGTTTCAGCTTCTTGTACATATGGGGAACTGAGAAACCTACTCCAG GCCACCACAGTCAAGACTTTACCATTGGTTGACTCCAAAGATTCAATGATCCTGCTGGGCTCTGTGGAAC GCTCAGAACTGCAGTCCCTCCTGCAGCGCCACCTGTGTGCAGAGCGAAGGTTGAAGGCTGCCCAGGACAT GGCTCGAAAGTTATCAGAGCTGCCTTATAATGGCAAGGCTCAGCTGGCTGGGGACTGGCATCCTGGTGGT CGACCTGAGTCCTTTGCCTTCGTAGATGAAGATGAAGATGAGGACCTCTCCAGGAAGATGGAGCTTCCAC TGACTCCAGCTCCTCCCCCTCCTTCCCCTCCTCCTCCACCTTCCCAGTTTCCTATTGCTCCATCAAACCC TGAAGAACCTAATGGGCCACTGCCCAGCCATAAACAGCCACCTGAAGCCTCAGACTCTGCAGATCAAAGA TCATCCACCTTCCAACGCCTGCTGCACTGCTTGCTAGGAAAAGCTCACTCCAAAAAGAAGAAAATAACAC AGGATTCAACAGATTTAGTGGATAACATGTCACCTGAAGAGATTGAGGCCTGGGAGCGGGAGCAGCTGAG CCAGCCTGTGTGCTTTGATTGCTGCTGCATCGACCAGTCTCCCTTCCAGCTGGTGGAGCAGACAACCCTG CACAAGACTCATACACTCTTCTCACTTCTTGGCCTCCATCTTGCCTATGTGACCAGCATGGGGAAGCTCA GAGGTGTCTTGGCACTAGAAGAGCTACAGAAAGCTATTGAGGGCCACACCAAATCTGGGGTGCAGCTTCG CCCTCCACTTGCCAGCTTCCGGAATACAACTTCAATCCGGAAGACTCCTGGGGGGCCACCCCCTCCTGCA GAGGGCTGGAATGTACCTGAGGATGGAGATGGGGCTCCTGGAAGAGAAGTCATGGTTCCTACCATGCCAG AGACTCCTGTCCCACCACCATCTCCAGAGGCCCCTTCCTGCCTGGCCCCAGCCAGAGCGGAGGGTGAGCT GGAGGAACTGGAGATGGTGGGGAGCCTAGAGCCTGAGGAGGAGCTGGCTGACATCTTGCATGGCCCCAGT CTGCGGTCCACTGATGAGGAAGATGAGGACGAGCTGATCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220414 representing NM_013491
Red=Cloning site Green=Tags(s) MERSQSQRHGGEQSWWGSAPQYQYMPFEHCTSYGLPSENGGLQHRPRKDMGPRHNAHPTQIYGHQKEQYS YKAQDGGMPKKMGSSSTMDSLDEDHYSKCQDCVHRLGRVLRRKLGEDWIFLVLLGLLMALVSWCMDYVSA KSLQAYKWTYAQMKPSLPLQYLAWVTFPLILILFSALFCQLISPQAVGSGIPEMKTILRGVVLKEYLTLK AFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSMFSGVYEQPYYYTDILTVGCAVGVGCCFG TPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPA FAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMP REAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVL GAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMM VAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQ ATTVKTLPLVDSKDSMILLGSVERSELQSLLQRHLCAERRLKAAQDMARKLSELPYNGKAQLAGDWHPGG RPESFAFVDEDEDEDLSRKMELPLTPAPPPPSPPPPPSQFPIAPSNPEEPNGPLPSHKQPPEASDSADQR SSTFQRLLHCLLGKAHSKKKKITQDSTDLVDNMSPEEIEAWEREQLSQPVCFDCCCIDQSPFQLVEQTTL HKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSIRKTPGGPPPPA EGWNVPEDGDGAPGREVMVPTMPETPVPPPSPEAPSCLAPARAEGELEELEMVGSLEPEEELADILHGPS LRSTDEEDEDELIL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013491 |
| ORF Size | 2982 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013491.3 |
| RefSeq Size | 3545 bp |
| RefSeq ORF | 2985 bp |
| Locus ID | 12723 |
| UniProt ID | Q64347 |
| Gene Summary | Voltage-gated chloride channel (By similarity). Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction (Probable) (PubMed:8119941).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222986 | Clcn1 (untagged) - Mouse chloride channel 1 (Clcn1), (10ug) |
CNY 10,928.00 |
|
| MR220414 | Clcn1 (Myc-DDK-tagged) - Mouse chloride channel 1 (Clcn1) |
CNY 10,920.00 |
|
| MR220414L3 | Lenti ORF clone of Clcn1 (Myc-DDK-tagged) - Mouse chloride channel 1 (Clcn1) |
CNY 11,780.00 |
|
| MR220414L4 | Lenti ORF clone of Clcn1 (mGFP-tagged) - Mouse chloride channel 1 (Clcn1) |
CNY 11,780.00 |
