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Coasy (NM_027896) Mouse Tagged ORF Clone
CAT＃: MG223307
- TrueORF®
Coasy (tGFP-tagged) - Mouse Coenzyme A synthase (Coasy) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
"NM_027896" in other vectors (4)
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CNY 3,520.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1300003G02Rik; Dpck; Ppat; Ukr1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223307 representing NM_027896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTGTTCCGCTCAGGCCTCCTGGTGCTGACAACGCCGCTGGCCACCCTGGCCGCACGCCTGCCTC CCATCCTGACCTCGGCGTCCCGCCTGGTGAATCACACCCTCTATGTGCACCTGCAGCCGGGCATGAACCT GGGGGGACCCGCTCAGCCTCAGGCCAGCCCTGTGCAGGCCACATTTGAGGTTCTGGATTTCATCACGCAC CTCTACACTGGTGCCGACCTGCACAGGCACCTGGACGTCAGAATTCTACTGACCAATATCCAAACCAAGA GCACCTTTCTCCCTGTGCTGTCCTCAGTCCAGAATCTGGCCCACCCTCCAGAAGTGGTTCTGACGGACTT CCAGACCCTGGACGGGAGCCAGTACAACCCTGTTAAACAACAGCTAGAGCGTTATGCCACCAGTTGTTAC AGCTGTTCTCCACAGCTGGCTTCTGTGTTGCTGTATCCGGATTATGGAACTGGAGAGCTGCCTTTGGAGC CCCCGAATGCCCTCTTACCCTCTACTATAAGGCCGGCTTCCCCGGTTGCCAGATCTCCAAGGCAGCCTGT GCGTGGCTACCACCGTGGGGCCGTGGGTGGCACTTTTGACCGCTTGCACAATGCCCACAAAGTGTTGCTC AGTGTTGCATGTGTACTGGCCCAGGAGCAGCTTGTGGTGGGAGTAGCTGACAAAGATCTGTTGAAGAGCA AGTTGCTCCCCGAGCTGCTCCAGCCGTATGCAGAGCGCGTGGAGCATCTGACTGAGTTCCTGGTGGACAT CAAGCCCTCCCTGACCTTTGAACTCGTCCCCCTGCTGGATCCCTATGGGCCTGCTGGGTCCGACCCCACC TTGGAGTTCCTGGTGGTCAGCGAGGAGACCTACCGTGGGGGGATGGCTGTCAACCGCTTCCGCCTTGAGA ATGGCAAAGAAGAACTTGCTTTGTACCAGATACAACTGCTGAAGGACCAGAGTCACAATGAAAATGAGGA GGACAAGGTCAGCTCCTCCAGCTTTCGCCAGCGAATCCTGGGAAACCTGCTTCAGCCTCCAAATGAGAGG CCGGAGCTCCCGTCAGGTCTCTATGTGCTCGGGCTCACAGGCATCAGTGGCTCTGGGAAAAGCTCGGTAG CTCAGCGGCTGAAGAACTTGGGAGCATACATCATCGACAGCGACCATCTGGGCCATCGGGCCTATGCTCC TGGGGGTCCTGCCTACCAGCCTGTGGTGGAGGCCTTTGGAACAGATATTCTCCATAAAGATGGCACCATC AATAGGAAGGTCCTGGGCAGCCGGGTGTTTGGGAACAAGAAGCAGATGAAGATCCTCACAGATATTGTGT GGCCAGTTATCGCAAAGCTGGCCCGGGAGGAGATGGATGTGGCTGTGGCTAAGGGAAAAACTCTGTGTGT AATCGATGCTGCTATGCTGCTTGAAGCTGGCTGGCAGAGTATGGTACACGAGGTGTGGACCGTCGTCATC CCTGAGACTGAGGCTGTTCGACGCATTGTTGAGAGGGATGGACTAAGTGAGGCAGCCGCTCAGAGCCGGC TGCAGAGCCAGATGAGCGGCCAGCAGCTGGTAGAGCAGAGCAACGTGGTTCTGAGCACCTTGTGGGAGTC ACATGTCACACAGAGCCAGGTGGAAAAGGCGTGGAATCTTCTACAGAAGCGCCTCCCTAAGGCTTACCAG ACCAGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223307 representing NM_027896
Red=Cloning site Green=Tags(s) MAVFRSGLLVLTTPLATLAARLPPILTSASRLVNHTLYVHLQPGMNLGGPAQPQASPVQATFEVLDFITH LYTGADLHRHLDVRILLTNIQTKSTFLPVLSSVQNLAHPPEVVLTDFQTLDGSQYNPVKQQLERYATSCY SCSPQLASVLLYPDYGTGELPLEPPNALLPSTIRPASPVARSPRQPVRGYHRGAVGGTFDRLHNAHKVLL SVACVLAQEQLVVGVADKDLLKSKLLPELLQPYAERVEHLTEFLVDIKPSLTFELVPLLDPYGPAGSDPT LEFLVVSEETYRGGMAVNRFRLENGKEELALYQIQLLKDQSHNENEEDKVSSSSFRQRILGNLLQPPNER PELPSGLYVLGLTGISGSGKSSVAQRLKNLGAYIIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGTI NRKVLGSRVFGNKKQMKILTDIVWPVIAKLAREEMDVAVAKGKTLCVIDAAMLLEAGWQSMVHEVWTVVI PETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSNVVLSTLWESHVTQSQVEKAWNLLQKRLPKAYQ TRN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027896 |
| ORF Size | 1689 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027896.4, NP_082172.2 |
| RefSeq Size | 2386 bp |
| RefSeq ORF | 1692 bp |
| Locus ID | 71743 |
| Gene Summary | This gene encodes the bifunctional protein coenzyme A (CoA) synthase which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this protein catalyzes the conversion of phosphopantetheine into dephospho-CoA while its dephospho-CoA kinase domain completes the final step by phosphorylating dephospho-CoA to form CoA. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219096 | Coasy (untagged) - Mouse Coenzyme A synthase (Coasy), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,220.00 |
|
| MR223307 | Coasy (Myc-DDK-tagged) - Mouse Coenzyme A synthase (Coasy), nuclear gene encoding mitochondrial protein |
CNY 3,230.00 |
|
| MR223307L3 | Lenti ORF clone of Coasy (Myc-DDK-tagged) - Mouse Coenzyme A synthase (Coasy), nuclear gene encoding mitochondrial protein |
CNY 5,130.00 |
|
| MR223307L4 | Lenti ORF clone of Coasy (mGFP-tagged) - Mouse Coenzyme A synthase (Coasy), nuclear gene encoding mitochondrial protein |
CNY 5,130.00 |
