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Myf5 (NM_008656) Mouse Tagged ORF Clone
CAT＃: MG225481
- TrueORF®
Myf5 (tGFP-tagged) - Mouse myogenic factor 5 (Myf5), (10ug)
ORF Plasmid: DDK
"NM_008656" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | B130010J22Rik; bHLHc2; Myf-5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225481 representing NM_008656
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATGACGGACGGCTGCCAGTTCTCCCCTTCTGAGTACTTCTATGAAGGCTCCTGTATCCCCTCAC CAGAGGATGAGTTTGGGGACCAGTTTGAGCCAAGAGTAGCAGCCTTCGGAGCACACAAAGCTGAGCTGCA GGGCTCAGACGATGAGGAGCACGTGCGTGCACCTACCGGCCACCACCAGGCTGGCCACTGCCTCATGTGG GCCTGCAAAGCTTGCAAGAGGAAGTCCACTACCATGGATCGGCGCAAGGCCGCCACCATGCGCGAGCGTA GACGCCTGAAGAAGGTCAACCAAGCTTTCGAGACGCTCAAGAGGTGCACCACCACCAACCCTAACCAGAG ACTCCCCAAGGTGGAGATCCTCAGGAATGCCATCCGCTACATTGAGAGCCTCCAGGAGCTGCTGAGGGAA CAGGTGGAGAACTATTACAGCCTGCCGGGACAGAGCTGCTCTGAGCCCACCAGCCCCACCTCCAACTGCT CTGACGGCATGCCTGAATGTAACAGCCCTGTCTGGTCCCGAAAGAACAGCAGCTTTGACAGCATCTACTG TCCTGATGTATCAAATGCATGTGCTGCAGATAAAAGCTCCGTGTCCAGCTTGGATTGCTTGTCCAGCATT GTGGATCGGATCACGTCTACAGAGCCATCCGAGCTGGCTCTTCAGGACACAGCTTCCCTCTCTCCAGCGA CCAGCGCCAACTCACAGCCTGCTACCCCGGGACCCTCCAGCTCCAGACTTATCTATCACGTATTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225481 representing NM_008656
Red=Cloning site Green=Tags(s) MDMTDGCQFSPSEYFYEGSCIPSPEDEFGDQFEPRVAAFGAHKAELQGSDDEEHVRAPTGHHQAGHCLMW ACKACKRKSTTMDRRKAATMRERRRLKKVNQAFETLKRCTTTNPNQRLPKVEILRNAIRYIESLQELLRE QVENYYSLPGQSCSEPTSPTSNCSDGMPECNSPVWSRKNSSFDSIYCPDVSNACAADKSSVSSLDCLSSI VDRITSTEPSELALQDTASLSPATSANSQPATPGPSSSRLIYHVL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008656 |
| ORF Size | 765 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008656.5, NP_032682.1 |
| RefSeq Size | 2083 bp |
| RefSeq ORF | 768 bp |
| Locus ID | 17877 |
| UniProt ID | P24699 |
| Gene Summary | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209001 | Myf5 (untagged) - Mouse myogenic factor 5 (Myf5), (10ug) |
CNY 3,990.00 |
|
| MR225481 | Myf5 (Myc-DDK-tagged) - Mouse myogenic factor 5 (Myf5) |
CNY 2,400.00 |
|
| MR225481L3 | Lenti ORF clone of Myf5 (Myc-DDK-tagged) - Mouse myogenic factor 5 (Myf5) |
CNY 4,470.00 |
|
| MR225481L4 | Lenti ORF clone of Myf5 (mGFP-tagged) - Mouse myogenic factor 5 (Myf5) |
CNY 4,470.00 |
