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Ide (NM_031156) Mouse Tagged ORF Clone
CAT＃: MG225567
- TrueORF®
Ide (tGFP-tagged) - Mouse insulin degrading enzyme (Ide), (10ug)
ORF Plasmid: DDK
"NM_031156" in other vectors (4)
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CNY 9,690.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1300012G03Rik; 4833415K22Rik; AA675336; AI507533 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225567 representing NM_031156
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAACGGGCTCGTGTGGCTGCTGCACCCCGCGCTGCCCGGCACCTTGCGCTCCATCCTCGGCGCCC GCCCGCCGCCCGCGAAGCGACTGTGTGGATTCCCAAAACAGACTTACAGCACAATGAGTAATCCGGCCAT CCAGAGAATAGAAGACCAAATTGTCAAGTCTCCTGAAGACAAACGGGAATACCGTGGACTAGAGCTGGCC AATGGCATCAAAGTGCTTCTCATCAGCGATCCCACCACAGACAAGTCCTCAGCGGCCCTCGATGTGCACA TAGGTTCACTGTCAGACCCTCCAAATATTCCTGGCTTAAGTCATTTTTGTGAACATATGCTGTTTTTGGG AACCAAGAAATATCCTAAAGAAAATGAATATAGCCAGTTTCTCAGTGAACATGCTGGAAGTTCAAATGCA TTCACCAGTGGAGAACACACCAATTATTATTTCGATGTTTCCCATGAACACTTGGAAGGAGCCCTGGACA GGTTTGCGCAGTTTTTCCTGTGCCCCTTGTTTGATGCAAGTTGTAAAGACAGAGAGGTGAACGCTGTCGA TTCAGAACATGAGAAGAATGTGATGAACGATGCCTGGAGACTCTTCCAGCTGGAAAAGGCTACGGGGAAC CCCAAACACCCCTTCAGCAAATTTGGGACAGGAAACAAATATACTCTAGAGACTCGGCCCAACCAAGAAG GCATCGACGTAAGGGAAGAGCTCTTGAAATTTCACTCTACGTATTATTCGTCCAATCTGATGGCGATTTG TGTTTTAGGTCGAGAATCCTTAGACGACCTCACTAACCTGGTGGTGAAGCTGTTCTCCGAGGTGGAGAAC AAGAACGTTCCTCTGCCTGAGTTCCCTGAGCACCCTTTCCAGGAAGAGCATCTCAGGCAACTTTATAAAA TAGTACCCATTAAGGATATTAGAAATCTTTATGTGACATTTCCCATACCAGACCTTCAGCAGTACTACAA ATCCAATCCTGGTCATTATCTTGGTCATCTAATTGGGCACGAAGGTCCTGGAAGCCTGTTGTCAGAACTC AAATCAAAGGGCTGGGTGAATACCCTGGTTGGTGGACAGAAGGAAGGAGCCCGAGGTTTTATGTTTTTTA TCATTAATGTGGACTTAACTGAAGAAGGGTTATTGCACGTTGAAGATATAATTTTGCACATGTTTCAATA CATTCAGAAGCTACGTGCAGAAGGACCTCAAGAATGGGTTTTCCAAGAGTGCAAGGACTTGAATGCTGTT GCTTTCAGATTTAAAGATAAAGAGAGACCACGAGGCTATACGTCCAAGATTGCAGGAAAGTTGCACTATT ATCCCCTAAATGGAGTGCTCACAGCTGAATATCTATTGGAGGAATTTAGACCCGACTTGATAGACATGGT TCTTGATAAACTCAGACCAGAAAATGTGCGGGTGGCAATAGTTTCCAAATCATTTGAAGGGAAAACTGAC CGCACAGAGCAGTGGTATGGAACACAGTATAAGCAAGAAGCTATCCCAGAGGACATCATTCAGAAATGGC AAAATGCTGACCTTAATGGAAAATTTAAGCTTCCAACAAAGAATGAATTTATTCCCACAAATTTTGAGAT TTTGTCATTAGAAAAAGATGCAACACCATACCCTGCTCTTATTAAGGATACAGCCATGAGTAAGCTGTGG TTCAAACAAGATGATAAATTTTTCTTGCCGAAAGCTTGTCTCAACTTTGAATTTTTCAGCCCGTTTGCTT ATGTGGACCCCTTGCACTGTAACATGGCCTATTTGTACCTTGAGCTCCTCAAAGACTCACTCAACGAGTA TGCATATGCAGCAGAGCTAGCAGGCCTGAGCTATGACCTCCAAAATACCATCTATGGGATGTATCTCTCA GTGAAAGGTTACAATGACAAACAGCCAATTTTGCTGAAGAAGATCACCGAGAAAATGGCTACTTTTGAGA TTGATAAGAAGAGATTTGAAATTATCAAAGAGGCCTATATGCGATCTCTTAATAACTTCCGGGCTGAGCA GCCTCACCAGCATGCCATGTACTACCTCCGCTTGCTGATGACTGAAGTGGCCTGGACTAAAGATGAGCTA AAAGAAGCCCTTGACGATGTTACCCTCCCCCGACTTAAGGCCTTCATACCTCAGCTGCTGTCACGGCTGC ATATTGAAGCACTTCTCCATGGAAACATAACCAAGCAGGCTGCCTTAGGAGTTATGCAGATGGTAGAAGA CACTCTTATCGAGCATGCTCACACCAAACCTCTCCTTCCAAGTCAGCTAGTCCGGTACAGAGAAGTCCAG CTCCCTGACAGAGGATGGTTTGTTTATCAGCAGAGGAATGAAGTTCACAATAACTGCGGCATCGAGATCT ACTACCAGACAGACATGCAGAGCACCTCGGAGAACATGTTCCTGGAGCTGTTTTGCCAGATTATCTCGGA GCCTTGCTTCAATACCCTGCGCACCAAGGAGCAGCTTGGCTATATTGTCTTCAGTGGACCCCGTCGGGCC AACGGTATCCAAGGCTTGCGCTTCATCATCCAGTCAGAAAAACCACCCCACTACCTGGAGAGCAGAGTAG AAGCCTTCTTGATTACCATGGAAAAGGCCATAGAGGACATGACAGAGGAGGCGTTCCAAAAACACATTCA AGCACTAGCGATTCGGCGACTCGACAAACCAAAGAAACTCTCTGCGGAGTGTGCTAAATACTGGGGGGAA ATCATCTCGCAGCAGTACAATTACGACAGAGATAACATAGAAGTTGCATATTTAAAGACACTTACCAAGG ACGATATCATCAGATTCTACCAGGAAATGTTGGCTGTCGACGCACCGAGGAGACATAAAGTGTCTGTCCA TGTTCTTGCCAGGGAAATGGATTCTTGTCCTGTTGTTGGAGAGTTCCCCTCTCAGAATGATATAAACTTG TCCGAAGCGCCACCCTTGCCACAACCTGAAGTGATTCACAACATGACTGAGTTCAAGCGTGGCCTGCCAC TGTTTCCCCTCGTGAAACCACATATTAACTTCATGGCTGCAAAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225567 representing NM_031156
Red=Cloning site Green=Tags(s) MRNGLVWLLHPALPGTLRSILGARPPPAKRLCGFPKQTYSTMSNPAIQRIEDQIVKSPEDKREYRGLELA NGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNA FTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGN PKHPFSKFGTGNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVEN KNVPLPEFPEHPFQEEHLRQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLLSEL KSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAV AFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLRPENVRVAIVSKSFEGKTD RTEQWYGTQYKQEAIPEDIIQKWQNADLNGKFKLPTKNEFIPTNFEILSLEKDATPYPALIKDTAMSKLW FKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLS VKGYNDKQPILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDEL KEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAHTKPLLPSQLVRYREVQ LPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRA NGIQGLRFIIQSEKPPHYLESRVEAFLITMEKAIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGE IISQQYNYDRDNIEVAYLKTLTKDDIIRFYQEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPSQNDINL SEAPPLPQPEVIHNMTEFKRGLPLFPLVKPHINFMAAKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031156 |
| ORF Size | 5084 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031156.4 |
| RefSeq ORF | 3060 bp |
| Locus ID | 15925 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223204 | Ide (untagged) - Mouse insulin degrading enzyme (Ide), (10ug) |
CNY 13,110.00 |
|
| MR225567 | Ide (Myc-DDK-tagged) - Mouse insulin degrading enzyme (Ide) |
CNY 7,096.00 |
|
| MR225567L3 | Lenti ORF clone of Ide (Myc-DDK-tagged) - Mouse insulin degrading enzyme (Ide) |
CNY 10,740.00 |
|
| MR225567L4 | Lenti ORF clone of Ide (mGFP-tagged) - Mouse insulin degrading enzyme (Ide) |
CNY 10,740.00 |
