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Kcnc1 (NM_008421) Mouse Tagged ORF Clone
CAT＃: MG225883
- TrueORF®
Kcnc1 (tGFP-tagged) - Mouse potassium voltage gated channel Shaw-related subfamily member 1 (Kcnc1) transcript variant B, (10ug)
ORF Plasmid: DDK
"NM_008421" in other vectors (4)
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CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C230009H10Rik; Kcr2-1; KShIIIB; Kv3.1; KV4; NGK2; Shaw |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225883 representing NM_008421
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCAAGGGGACGAGAGCGAGCGCATCGTGATCAACGTGGGCGGCACGCGCCACCAGACGTACCGCT CGACGCTGCGCACGCTGCCCGGCACGCGGCTTGCCTGGCTGGCAGAGCCGGACGCCCACAGCCACTTCGA CTATGACCCGCGTGCCGACGAGTTCTTCTTCGACCGCCACCCGGGCGTCTTCGCTCACATCCTGAACTAT TACCGCACCGGCAAGCTTCACTGCCCGGCCGACGTGTGCGGGCCGCTCTACGAGGAGGAGCTGGCCTTCT GGGGCATCGACGAGACGGACGTGGAGCCCTGCTGCTGGATGACCTATCGCCAGCACCGAGACGCTGAGGA GGCGCTGGACAGCTTTGGCGGTGCGCCCTTGGACAACAGCGCCGACGACGCGGACGCCGACGGCCCCGGC GACTCGGGCGACGGCGAGGACGAGCTGGAGATGACCAAGAGATTGGCACTCAGTGACTCCCCAGATGGCC GGCCTGGCGGCTTCTGGCGCCGCTGGCAACCGCGCATCTGGGCGCTGTTCGAGGACCCCTACTCATCCCG CTACGCGCGGTATGTGGCCTTTGCCTCCCTCTTCTTCATCCTGGTCTCCATCACAACCTTCTGTCTGGAG ACTCACGAGCGCTTCAACCCCATCGTGAACAAGACCGAAATCGAGAACGTTCGAAACGGCACCCAAGTGC GGTACTACCGGGAAGCAGAGACGGAGGCCTTCCTCACCTACATCGAGGGCGTCTGCGTGGTCTGGTTCAC CTTCGAGTTCCTCATGCGTGTCGTCTTCTGCCCCAACAAGGTGGAATTCATCAAGAACTCCCTCAATATC ATTGACTTTGTGGCCATTCTCCCCTTCTACCTGGAGGTGGGCCTAAGCGGCCTGTCCTCAAAAGCCGCCA AGGACGTTCTGGGCTTCCTGCGCGTCGTCCGCTTCGTGCGCATCCTGCGCATCTTCAAGCTGACCCGCCA CTTCGTGGGCCTGAGGGTCCTGGGCCACACGCTCCGTGCCAGCACCAACGAGTTCCTGCTGCTTATCATC TTCCTGGCCCTGGGAGTGCTCATCTTTGCCACCATGATCTACTACGCCGAGAGGATAGGGGCACAGCCCA ATGACCCCAGCGCCAGCGAACACACACACTTTAAAAACATCCCCATCGGCTTCTGGTGGGCTGTGGTCAC CATGACGACACTGGGCTATGGAGACATGTATCCCCAGACGTGGTCTGGAATGCTGGTGGGAGCCTTGTGT GCTCTGGCTGGTGTGCTGACCATTGCCATGCCGGTGCCTGTCATCGTGAACAATTTTGGGATGTACTACT CTTTAGCCATGGCTAAGCAGAAACTACCAAAGAAAAAAAAGAAGCATATTCCGCGGCCACCACAGCTGGG ATCTCCCAATTATTGTAAATCTGTCGTAAACTCTCCACACCACAGTACTCAGAGTGACACATGCCCGCTG GCCCAGGAAGAAATTTTAGAAATTAACAGAGCAGGTAGGAAACCTCTCAGAGGCATGTCGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225883 representing NM_008421
Red=Cloning site Green=Tags(s) MGQGDESERIVINVGGTRHQTYRSTLRTLPGTRLAWLAEPDAHSHFDYDPRADEFFFDRHPGVFAHILNY YRTGKLHCPADVCGPLYEEELAFWGIDETDVEPCCWMTYRQHRDAEEALDSFGGAPLDNSADDADADGPG DSGDGEDELEMTKRLALSDSPDGRPGGFWRRWQPRIWALFEDPYSSRYARYVAFASLFFILVSITTFCLE THERFNPIVNKTEIENVRNGTQVRYYREAETEAFLTYIEGVCVVWFTFEFLMRVVFCPNKVEFIKNSLNI IDFVAILPFYLEVGLSGLSSKAAKDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLII FLALGVLIFATMIYYAERIGAQPNDPSASEHTHFKNIPIGFWWAVVTMTTLGYGDMYPQTWSGMLVGALC ALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKKKKHIPRPPQLGSPNYCKSVVNSPHHSTQSDTCPL AQEEILEINRAGRKPLRGMSI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008421 |
| ORF Size | 1533 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008421.3, NP_032447.1 |
| RefSeq Size | 7788 bp |
| RefSeq ORF | 1536 bp |
| Locus ID | 16502 |
| UniProt ID | P15388 |
| Gene Summary | Voltage-gated potassium channel that plays an important role in the rapid repolarization of fast-firing brain neurons. The channel opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient (PubMed:2599109, PubMed:1400413). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNC2, and possibly other family members as well. Contributes to fire sustained trains of very brief action potentials at high frequency in pallidal neurons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217139 | Kcnc1 (untagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 1 (Kcnc1), transcript variant B, (10ug) |
CNY 6,096.00 |
|
| MR225883 | Kcnc1 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 1 (Kcnc1), transcript variant B |
CNY 5,704.00 |
|
| MR225883L3 | Lenti ORF clone of Kcnc1 (Myc-DDK-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 1 (Kcnc1), transcript variant B |
CNY 7,030.00 |
|
| MR225883L4 | Lenti ORF clone of Kcnc1 (mGFP-tagged) - Mouse potassium voltage gated channel, Shaw-related subfamily, member 1 (Kcnc1), transcript variant B |
CNY 7,030.00 |
