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Slc1a2 (NM_001077514) Mouse Tagged ORF Clone
CAT＃: MG226166
- TrueORF®
Slc1a2 (tGFP-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter) member 2 (Slc1a2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001077514" in other vectors (4)
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CNY 5,510.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1700091C19Rik; 2900019G14Rik; AI159670; Eaat2; GLT-1; GLT1; MGLT1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226166 representing NM_001077514
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCAACAGAGGGTGCCAACAATATGCCCAAGCAGGTAGAAGTGCGCATGCATGACAGCCACCTCA GCTCCGATGAGCCAAAGCACCGAAACCTGGGCATGCGCATGTGCGACAAGCTGGGGAAAAATCTCCTGCT CTCACTGACTGTGTTTGGTGTCATCCTGGGAGCAGTGTGTGGCGGGCTGCTTCGCTTGGCATCGCCCATC CACCCTGATGTGGTCATGTTGATAGCCTTCCCGGGGGACATACTCATGAGGATGCTGAAGATGCTCATCC TCCCTCTTATCATCTCCAGTTTAATCACAGGGTTGTCAGGCCTGGATGCTAAAGCCAGCGGCCGCCTAGG CACGAGAGCTATGGTGTATTACATGTCCACGACCATCATTGCCGCCGTGCTGGGGGTCATCCTGGTGTTG GCCATCCACCCAGGCAATCCCAAACTCAAGAAGCAGCTAGGGCCCGGGAAGAAGAACGACGAGGTGTCTA GCCTGGATGCCTTCCTGGATCTCATTAGAAATCTCTTCCCGGAGAACCTGGTGCAAGCCTGTTTCCAGCA GATTCAGACAGTGACAAAGAAAGTTCTGGTGGCACCTCCATCTGAGGAGGCCAATACCACCAAGGCGGTC ATCTCCATGTTGAATGAAACCATGAACGAGGCCCCTGAAGAAACTAAGATCGTTATCAAGAAGGGCCTGG AGTTCAAGGACGGGATGAACGTCTTAGGTCTGATCGGATTCTTTATTGCTTTCGGCATTGCCATGGGGAA GATGGGTGAACAGGCCAAGCTGATGGTGGAGTTCTTCAACATTCTGAATGAGATCGTGATGAAGTTAGTG ATCATGATCATGTGGTACTCCCCTCTGGGTATCGCCTGCTTGATTTGTGGGAAGATCATCGCCATCAAGG ACTTAGAAGTGGTTGCTAGGCAGCTGGGGATGTACATGATCACCGTGATCGTGGGCCTCATCATTCACGG GGGCATCTTTCTCCCCTTGATTTACTTTGTAGTGACCAGAAAAAATCCATTCTCCTTTTTTGCTGGCATA TTCCAAGCCTGGATCACTGCTCTGGGAACTGCTTCCAGTGCTGGAACTTTGCCTGTTACCTTCCGTTGCT TGGAAGATAATCTAGGGATTGACAAGCGTGTGACCAGATTCGTCCTCCCAGTCGGAGCAACCATTAACAT GGATGGCACAGCCCTTTACGAGGCTGTGGCAGCCATCTTCATAGCCCAAATGAATGGGGTCATCTTGGAT GGAGGTCAGATTGTGACTGTAAGCCTTACAGCCACCCTGGCGAGCATTGGTGCAGCCAGTATTCCCAGCG CCGGGCTGGTCACCATGCTCCTCATTCTCACAGCTGTGGGCCTGCCAACGGAGGATATCAGTCTGCTGGT GGCGGTGGACTGGCTGCTGGATAGAATGAGAACTTCAGTCAATGTGGTGGGCGATTCTTTTGGGGCTGGG ATTGTCTATCACCTTTCCAAGTCTGAGCTGGACACCATTGACTCCCAACACCGAATGCAGGAAGACATCG AAATGACCAAGACGCAGTCCATTTACGACGACAAGAACCACAGGGAAAGCAACTCTAATCAGTGTGTCTA TGCCGCACACAACTCTGTCGTAATAGATGAGTGCAAGGTAACTCTGGCGGCCAATGGAAAGTCAGCTGAC TGCAGTGTTGAGGAAGAACCTTGGAAACGTGAAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226166 representing NM_001077514
Red=Cloning site Green=Tags(s) MASTEGANNMPKQVEVRMHDSHLSSDEPKHRNLGMRMCDKLGKNLLLSLTVFGVILGAVCGGLLRLASPI HPDVVMLIAFPGDILMRMLKMLILPLIISSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVL AIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPSEEANTTKAV ISMLNETMNEAPEETKIVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGEQAKLMVEFFNILNEIVMKLV IMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMITVIVGLIIHGGIFLPLIYFVVTRKNPFSFFAGI FQAWITALGTASSAGTLPVTFRCLEDNLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVILD GGQIVTVSLTATLASIGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAG IVYHLSKSELDTIDSQHRMQEDIEMTKTQSIYDDKNHRESNSNQCVYAAHNSVVIDECKVTLAANGKSAD CSVEEEPWKREK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077514 |
| ORF Size | 1716 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001077514.4 |
| RefSeq Size | 11571 bp |
| RefSeq ORF | 1719 bp |
| Locus ID | 20511 |
| UniProt ID | P43006 |
| Gene Summary | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7698742, PubMed:7557442, PubMed:9373176). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (PubMed:9180080).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219173 | Slc1a2 (untagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 1, (10ug) |
CNY 7,320.00 |
|
| MR226166 | Slc1a2 (Myc-DDK-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 1 |
CNY 4,256.00 |
|
| MR226166L3 | Lenti ORF clone of Slc1a2 (Myc-DDK-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 1 |
CNY 6,940.00 |
|
| MR226166L4 | Lenti ORF clone of Slc1a2 (mGFP-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 2 (Slc1a2), transcript variant 1 |
CNY 6,940.00 |
