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Trim8 (NM_053100) Mouse Tagged ORF Clone
CAT＃: MG226681
- TrueORF®
Trim8 (tGFP-tagged) - Mouse tripartite motif-containing 8 (Trim8), (10ug)
ORF Plasmid: DDK
"NM_053100" in other vectors (4)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA408830; BB006755; GERP; Rnf27 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226681 representing NM_053100
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGAATTGGAAGAACTGCTTTGAGGAGGAGCTCATCTGCCCCATCTGCCTGCACGTTTTCGTGG AACCGGTGCAGCTGCCATGCAAACATAACTTCTGCCGGGGCTGCATCGGCGAGGCTTGGGCCAAGGACAG TGGCCTTGTGCGCTGCCCGGAGTGCAACCAGGCCTACAATCAGAAGCCGGGCCTGGAGAAGAACCTGAAG CTCACCAACATCGTGGAGAAGTTCAACGCCCTGCACGTGGAGAAGCCGCCGACGGCACTGCACTGCGTGT TCTGTCGCCGCGGCCCCCCGCTGCCGGCACAGAAGGTCTGCCTGCGCTGCGAGGCTCCCTGCTGCCAGTC TCACGTGCAGACGCACCTGCAGCAGCCCTCCACCGCCCGCGGGCACCTCCTGGTGGAGGCGGACGACGTG CGGGCCTGGAGCTGCCCGCAGCACAACGCCTACCGCCTGTACCACTGCGAGGCCGAGCAGGTGGCTGTGT GCCAGTACTGCTGCTACTACAGCGGCGCGCATCAGGGACACTCGGTGTGTGACGTGGAGATACGGAGGAA TGAGATACGGAAGATGCTCATGAAGCAGCAGGAGCGGCTGGAGGAGAGAGAGCAGGACATTGAGGACCAG CTGTACAAGCTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAGCCAGCTGAAGGAGGAGGTGCGGC TGCAGTACGAAAAGCTGCACCAGCTGCTGGATGAAGACTTGCGGCAGACAGTGGAGGTCCTGGACAAGGC GCAAGCCAAGTTCTGCAGCGAGAACGCGGCGCAGGCGCTGCACCTTGGGGAGCGCATGCAGGAGGCCAAG AAGCTGCTGGGCTCCCTGCAGCGTCTCTTCGACAAGACAGAGGACGTCGGCTTCATGAAGAACACCAAGT CTGTGAAAATCCTAATGGACAGGACCCAGACCTGCACGGGCAGCAGCCTTTCTCCCCCTAAGATCGGCCA CCTGAACTCCAAGCTGTTCCTGAACGAGGTGGCCAAGAAGGAGAAGCAGTTGCGGAAGATGCTAGAAGGT CCCTTCAGCACACCGGTGCCCTTCCTACAGAGCGTACCCCTGTACCCTTGTGGTGTGAACAGCTCTGGGG CCGAAAAGCGCAAGCACTCGACAGCCTTCCCTGAGGCTAGCTTCCTGGAGACTTCGTCAGGCCCAGTGGG CGGCCAGTACGGGGCAGCGGGCACAGCCAGCAGCGAGGGGCAGTCTGGGCAGCCCCTGGGTCCCTGCAGC TCCACGCAGCACTTGGTGGCTCTGCCAGGCGGTACCCAGCCAGTACACTCGAGTCCCGTGTTCCCCCCTT CGCAGTACCCCAACGGCTCCACCACCCAGCAACCCATGCTCCCCCAGTATGGCGGCCGCAAGATTCTCGT CTGTTCTGTGGACAACTGTTACTGCTCTTCCGTGGCCAACCACGGTGGCCACCAGCCGTACCCCCGATCC GGCCACTTCCCCTGGACAGTGCCCTCACAGGAGTATTCACACCCACTTCCACCCACACCCTCCGTCCCTC AGTCACTTCCTGGCCTGGCGGTCAGAGACTGGCTCGACGCTTCTCAGCAGCCTGGCCACCAGGATTTCTA CAGGGTGTATGGGCAGCCGTCCACCAAACACTATGTGACAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226681 representing NM_053100
Red=Cloning site Green=Tags(s) MAENWKNCFEEELICPICLHVFVEPVQLPCKHNFCRGCIGEAWAKDSGLVRCPECNQAYNQKPGLEKNLK LTNIVEKFNALHVEKPPTALHCVFCRRGPPLPAQKVCLRCEAPCCQSHVQTHLQQPSTARGHLLVEADDV RAWSCPQHNAYRLYHCEAEQVAVCQYCCYYSGAHQGHSVCDVEIRRNEIRKMLMKQQERLEEREQDIEDQ LYKLESDKRLVEEKVSQLKEEVRLQYEKLHQLLDEDLRQTVEVLDKAQAKFCSENAAQALHLGERMQEAK KLLGSLQRLFDKTEDVGFMKNTKSVKILMDRTQTCTGSSLSPPKIGHLNSKLFLNEVAKKEKQLRKMLEG PFSTPVPFLQSVPLYPCGVNSSGAEKRKHSTAFPEASFLETSSGPVGGQYGAAGTASSEGQSGQPLGPCS STQHLVALPGGTQPVHSSPVFPPSQYPNGSTTQQPMLPQYGGRKILVCSVDNCYCSSVANHGGHQPYPRS GHFPWTVPSQEYSHPLPPTPSVPQSLPGLAVRDWLDASQQPGHQDFYRVYGQPSTKHYVTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053100 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053100.2, NP_444330.2 |
| RefSeq Size | 3262 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 93679 |
| UniProt ID | Q99PJ2 |
| Gene Summary | Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218860 | Trim8 (untagged) - Mouse tripartite motif-containing 8 (Trim8), (10ug) |
CNY 7,030.00 |
|
| MR226681 | Trim8 (Myc-DDK-tagged) - Mouse tripartite motif-containing 8 (Trim8) |
CNY 4,104.00 |
|
| MR226681L3 | Lenti ORF clone of Trim8 (Myc-DDK-tagged) - Mouse tripartite motif-containing 8 (Trim8) |
CNY 6,650.00 |
|
| MR226681L4 | Lenti ORF clone of Trim8 (mGFP-tagged) - Mouse tripartite motif-containing 8 (Trim8) |
CNY 6,650.00 |
