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Kctd11 (NM_153143) Mouse Tagged ORF Clone
CAT＃: MR202806
- TrueORF®
Kctd11 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 11 (Kctd11)
ORF Plasmid: tGFP
"NM_153143" in other vectors (4)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AF465352; Ren |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202806 representing NM_153143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGGGCCATGTTTAGGGCTGACACCCTAATGCCAGCCAACCTTAACCCGCAAGGAGATGGCCATT ACTTCATCGACAGGGATGGCAAGGCTTTCCGGCACATCCTCAATTTTTTGCGGCTAGGCCGTCTGGACCT GCCCCGTGGGTACGGAGAAACTGCCCTTCTTAAGGCAGAGGCTGACTTCTACCAGATCCGGCCCCTCCTG GATGCCCTGCGGGAATTGGAAGCCTCTCGGGGTACACCTGCATCCACAGCCGCCCTACTCCATGCAGATG TAGATGTCAGCCCCCGCCAGGTGCACTTCTCCGCTCGAAGGGGCCCCCACCACTATGAGCTGAGCTCTGT CCAGGTGGACACCTTCCGAGCCAACCTCTTCTGCACTGACCCTGAGTGTCTGGCTGCCATGCGCAACAGA TTTGGTGTGGCCATTGGGGACAGGGCAGAAGGAGGTCCACATTTTCGGCTAGAGTGGGCCTCCCGCCCCC AGGAACTCCCTGAAGTAGAGTATCAAAGACTGGGGCTGCAGCCACTGTGGACTGGGGGGCCAGAAGATCG TCGGGAGGTAGCGAACACGCCTACATTCCTGGAGGAGGTGCTACGGGTGGCTCTGGAACATGGCTTCCGC CTGGACTCTGTCTTCCCAGACCCTGAAGACCTTCTGAACTCTAGATCCTTGCGCTTTGTGCGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202806 representing NM_153143
Red=Cloning site Green=Tags(s) MLGAMFRADTLMPANLNPQGDGHYFIDRDGKAFRHILNFLRLGRLDLPRGYGETALLKAEADFYQIRPLL DALRELEASRGTPASTAALLHADVDVSPRQVHFSARRGPHHYELSSVQVDTFRANLFCTDPECLAAMRNR FGVAIGDRAEGGPHFRLEWASRPQELPEVEYQRLGLQPLWTGGPEDRREVANTPTFLEEVLRVALEHGFR LDSVFPDPEDLLNSRSLRFVRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153143 |
| ORF Size | 696 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153143.4, NP_694783.1 |
| RefSeq Size | 2740 bp |
| RefSeq ORF | 699 bp |
| Locus ID | 216858 |
| UniProt ID | Q8K485 |
| MW | 26.7 kDa |
| Gene Summary | Plays a role as a marker and a regulator of neuronal differentiation; Up-regulated by a variety of neurogenic signals, such as retinoic acid, epidermal growth factor/EGF and NGFB/nerve growth factor. Induces apoptosis, growth arrest and the expression of cyclin-dependent kinase inhibitor CDKN1B. Plays a role as a tumor repressor and inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Acts as probable substrate-specific adapter for a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex towards HDAC1. Functions as antagonist of the Hedgehog pathway on cell proliferation and differentiation by affecting the nuclear transfer of transcription factor GLI1, thus maintaining cerebellar granule cells in undifferentiated state, this effect probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. When knock-down, Hedgehog antagonism is impaired and proliferation of granule cells is sustained. Activates the caspase cascade.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212654 | Kctd11 (untagged) - Mouse potassium channel tetramerisation domain containing 11 (Kctd11), (10ug) |
CNY 3,990.00 |
|
| MG202806 | Kctd11 (tGFP-tagged) - Mouse potassium channel tetramerisation domain containing 11 (Kctd11) |
CNY 2,850.00 |
|
| MR202806L3 | Lenti ORF clone of Kctd11 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 11 (Kctd11) |
CNY 4,750.00 |
|
| MR202806L4 | Lenti ORF clone of Kctd11 (mGFP-tagged) - Mouse potassium channel tetramerisation domain containing 11 (Kctd11) |
CNY 4,750.00 |
