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Usp12 (BC049274) Mouse Tagged ORF Clone
CAT＃: MR203192
- TrueORF®
Usp12 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 12 (cDNA clone MGC:55011 IMAGE:4500832)
ORF Plasmid: tGFP
"BC049274" in other vectors (4)
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CNY 2,400.00
CNY 6,365.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ubh1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203192 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACAAGATGCCCATGAATTCTTAAATTACCTACTCAATACAATTGCTGATATTCTACAAGAAGAAA GAAAACAGGAGAAGCAAAATGGCCGATTACGGAACGGAGACGTGGACAACGAGGATAACAACAGCACACC AGACCCAACCTGGGTCCACGAGATCTTTCAGGGAACATTAACTAATGAAACCAGGTGTCTTACTTGTGAA ACTATAAGCAGCAAAGATGAAGATTTTTTAGACCTTTCTGTTGACGTGGAACAAAACACATCAATTACTC ACTGCTTAAGAGGGTTCAGTAACACAGAAACTCTGTGCAGTGAATATAAGTACTACTGTGAAGAGTGCCG GAGCAAGCAGGAAGCACACAAGCGGATGAAGGTTAAGAAACTGCCCCTGATCCTGGCTCTGCACCTGAAG AGGTTTAAGTATATGGACCAGCTTCACCGGTACACGAAGCTTTCTTATCGCGTGGTCTTTCCTTTAGAGC TCCGTCTGTTCAACACGTCAGGAGATGCGACCAACCCTGACAGAATGTATGACCTGGTCGCCGTTGTGGT TCACTGTGGAAGTGGTCCCAACCGAGGCCATTATATTGCAATAGTTAAGAGTCATGATTTCTGGCTGTTG TTTGATGATGACATTGTAGAAAAAATCGATGCCCAAGCTATTGAAGAATTCTACGGATTGACATCTGACA TCTCCAAGAACTCGGAGTCTGGCTACATCCTTTTCTATCAGTCTCGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203192 protein sequence
Red=Cloning site Green=Tags(s) MQQDAHEFLNYLLNTIADILQEERKQEKQNGRLRNGDVDNEDNNSTPDPTWVHEIFQGTLTNETRCLTCE TISSKDEDFLDLSVDVEQNTSITHCLRGFSNTETLCSEYKYYCEECRSKQEAHKRMKVKKLPLILALHLK RFKYMDQLHRYTKLSYRVVFPLELRLFNTSGDATNPDRMYDLVAVVVHCGSGPNRGHYIAIVKSHDFWLL FDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC049274 |
| ORF Size | 750 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC049274, AAH49274 |
| RefSeq Size | 3928 bp |
| RefSeq ORF | 752 bp |
| Locus ID | 22217 |
| MW | 29.2 kDa |
| Gene Summary | Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217763 | Usp12 (untagged) - Mouse ubiquitin specific peptidase 12 (cDNA clone MGC:55011 IMAGE:4500832), (10ug) |
CNY 6,940.00 |
|
| MG203192 | Usp12 (tGFP-tagged) - Mouse ubiquitin specific peptidase 12 (cDNA clone MGC:55011 IMAGE:4500832) |
CNY 2,850.00 |
|
| MR203192L3 | Lenti ORF clone of Usp12 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 12 (cDNA clone MGC:55011 IMAGE:4500832) |
CNY 4,750.00 |
|
| MR203192L4 | Lenti ORF clone of Usp12 (mGFP-tagged) - Mouse ubiquitin specific peptidase 12 (cDNA clone MGC:55011 IMAGE:4500832) |
CNY 4,750.00 |
