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Lefty2 (NM_177099) Mouse Tagged Lenti ORF Clone
CAT＃: MR205670L3
- LentiORF®
Lenti ORF clone of Lefty2 (Myc-DDK-tagged) - Mouse left-right determination factor 2 (Lefty2)
"NM_177099" in other vectors (4)
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CNY 4,750.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6030463A22Rik; Eba; Ebaf; Left; Lefta; Leftb; lefty-2; lefty-B |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR205670).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177099 |
| ORF Size | 1107 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177099.3 |
| RefSeq Size | 2534 bp |
| RefSeq ORF | 1107 bp |
| Locus ID | 320202 |
| UniProt ID | P57785 |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This protein is also important in self-renewal and differentiation of mouse embryonic stem cells. Mice lacking a functional copy of this gene exhibit defects in axial and left-right patterning. [provided by RefSeq, Aug 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201921 | Lefty2 (untagged) - Mouse left-right determination factor 2 (Lefty2), (10ug) |
CNY 2,000.00 |
|
| MG205670 | Lefty2 (tGFP-tagged) - Mouse Left-right determination factor 2 (Lefty2) |
CNY 3,140.00 |
|
| MR205670 | Lefty2 (Myc-DDK-tagged) - Mouse left-right determination factor 2 (Lefty2) |
CNY 2,850.00 |
|
| MR205670L4 | Lenti ORF clone of Lefty2 (mGFP-tagged) - Mouse left-right determination factor 2 (Lefty2) |
CNY 4,750.00 |
