Sp8 (BC082582) Mouse Tagged ORF Clone
CAT＃: MR207072
- TrueORF®
Sp8 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 8 (cDNA clone MGC:105193 IMAGE:30653287)
ORF Plasmid: tGFP
"BC082582" in other vectors (4)
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CNY 5488.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D930049B17Rik; mBtd |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207072 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACTTCACTTCTAGGGGAAGAACCGAGGTTGGGATCCACTCCTCTGGCCATGCTTGCTGCTACCT GTAATAAGATCGGCAGCCCCAGCCCGTCTCCCTCCTCCCTGTCGGACAGCTCTTCTTCCTTCGGTAAAGG CTTCCACCCTTGGAAACGCTCCTCGTCTTCCTCCTCCGGCAGCTGCAACGTAGTGGGTTCCAGTCTCTCT AGCTTCGGTGTGTCGGGGGCCTCCCGGAACGGCGGCTCCTCCTCAGCCGCAGCGGCGGCAGCGGCGGCAG CAGCGGCGGCCGCAGCCCTGGTGTCCGACTCTTTTAGCTGCGGAGGCTCGCCCGGCTCCAGCGCCTTCTC TCTCACCTCCAGCAGCGCCGCCGCAGCGGCAGCAGCTGCGGCGGCTGCCGCTTCCAGCTCGCCCTTTGCC AACGACTACTCTGTGTTCCAGGCTCCGGGCGTCTCGGGGGGCAGCGGCGGCGGCGGCGGGGGAGGCGGCG GCGGTTCCGGAGCGCACTCGCAGGATAGCTCCCATCAGCCAGTGTTTATCTCCAAGGTGCACACGTCGGT GGACGGACTGCAGGGTATCTACCCGCGCGTGGGAATGGCGCACCCGTATGAGTCCTGGTTCAAGCCTTCG CACCCGGGCCTTGGCGCCGCGGCCGACGTGGGCTCCGCGGGCGCCTCCAGCTGGTGGGATGTGGGCGCGG GCTGGATCGACGTGCAGAACCCCAATGGGGCGGCAGCGCTGCCCGGCTCGCTGCACCCGGCGGCCGGGGG CCTTCAGACGTCGCTGCATTCACCGCTGGGAGGCTACAACTCGGATTATTCCGGCCTGAGCCACTCCGCT CGCCGCTACTCCGGCCGCGCCACTTGCGACTGCCCCAACTGCCAGGAGGCTGAGCGGCTGGGCCCAGCGG GAGCGAGCCTGCGTCGCAAAGGGCTGCATAGCTGCCACATCCCGGGCTGCGGCAAGGTGTACGGCAAGAC GTCGCACCTGAAGGCGCACCTGCGTTGGCACACCGGCGAGCGGCCGTTCGTGTGCAATTGGCTGTTCTGC GGTAAGCGCTTTACGCGCTCCGACGAGCTGCAGCGGCACCTGCGGACCCACACGGGCGAGAAGCGCTTCG CCTGCCCGGTGTGCAACAAACGCTTCATGCGCAGCGACCACCTGAGCAAGCACGTGAAGACACACAGTGG TGGCGGCGGCTCGGCGGGCTCCGGCGGCGGCAAGAAAGGCAGCGACACCGACAGCGAGCACAGCGCGGCG GGAAGCCCTCCTTGCCACTCCCCTGAGCTGTTGCAGCCTCCAGAGCCGGGCCACCGCAACGGCCTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207072 protein sequence
Red=Cloning site Green=Tags(s) MATSLLGEEPRLGSTPLAMLAATCNKIGSPSPSPSSLSDSSSSFGKGFHPWKRSSSSSSGSCNVVGSSLS SFGVSGASRNGGSSSAAAAAAAAAAAAAALVSDSFSCGGSPGSSAFSLTSSSAAAAAAAAAAAASSSPFA NDYSVFQAPGVSGGSGGGGGGGGGGSGAHSQDSSHQPVFISKVHTSVDGLQGIYPRVGMAHPYESWFKPS HPGLGAAADVGSAGASSWWDVGAGWIDVQNPNGAAALPGSLHPAAGGLQTSLHSPLGGYNSDYSGLSHSA RRYSGRATCDCPNCQEAERLGPAGASLRRKGLHSCHIPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFC GKRFTRSDELQRHLRTHTGEKRFACPVCNKRFMRSDHLSKHVKTHSGGGGSAGSGGGKKGSDTDSEHSAA GSPPCHSPELLQPPEPGHRNGLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC082582 |
| ORF Size | 1329 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC082582, AAH82582 |
| RefSeq Size | 4210 bp |
| RefSeq ORF | 1331 bp |
| Locus ID | 320145 |
| MW | 44.4 kDa |
| Gene Summary | Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218053 | Sp8 (untagged) - Mouse trans-acting transcription factor 8 (cDNA clone MGC:105193 IMAGE:30653287), (10ug) |
CNY 6940.00 |
|
| MG207072 | Sp8 (tGFP-tagged) - Mouse trans-acting transcription factor 8 (cDNA clone MGC:105193 IMAGE:30653287) |
CNY 7088.00 |
|
| MR207072L3 | Lenti ORF clone of Sp8 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 8 (cDNA clone MGC:105193 IMAGE:30653287) |
CNY 6370.00 |
|
| MR207072L4 | Lenti ORF clone of Sp8 (mGFP-tagged) - Mouse trans-acting transcription factor 8 (cDNA clone MGC:105193 IMAGE:30653287) |
CNY 6370.00 |
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