Nlrp6 (BC031139) Mouse Tagged ORF Clone

CAT#: MR207117

  • TrueORF®

Nlrp6 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485)

ORF Plasmid: DDK tGFP



  "BC031139" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,570.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Nlrp6 Rabbit pAb
    • 100 ul

CNY 2,640.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI504961; Avr; Nalp6; Navr; Navr/Avr; Non-AVR; Pypaf5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207117 representing BC031139
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAAGTCTGCAGGCACCAATGGACCCCGGGTTCAGGGAGAGCTGAGAACGCTGTGTCGCCTGGCCA
GGGAGGGCATCCTGGATCATCATAAAGCACAATTCTCAGAAGAGGACCTGGAGAAATTGAAGCTTCGGGG
TTCCCAAGTTCAGACAATATTTCTCAACAAGAAAGAGATACCAGGAGTGCTAAAAACTGAGGTCACCTAC
CAGTTCATTGACCAGAGCTTCCAGGAGTTCTTGGCTGCATTGTCATACCTACTAGAAGCTGAGCGAACTC
CGGGGACGCCCGCAGGCGGTGTGCAGAAGCTCCTGAACTCTGACGCGGAGCTACGTGGTCATCTTGCACT
GACCACCCGATTCCTCTTTGGCCTGCTAAATACAGAGGGGCTTCGTGACATTGGAAACCATTTTGGCTGT
GTGGTGCCAGATCATGTGAAAAAGGACACCCTGCGGTGGGTACAAGGACAGAGCCACCCCAAGGGGCCAC
CAGTAGGGGCAAAAAAGACTGCTGAGCTGGAGGACATCGAGGACGCAGAGGAGGAGGAGGAGGAAGAGGA
GGACCTCAATTTTGGACTGGAGCTGCTGTACTGCCTGTATGAGACACAGGAGGAGGATTTTGTTCGACAG
GCTCTCAGCAGCCTTCCAGAGATAGTACTGGAGCGAGTCAGGTTGACCCGCATGGACCTTGAAGTTCTGA
ATTACTGCGTGCAGTGCTGCCCAGATGGCCAGGCTCTGAGACTGGTGAGCTGTGGGCTGGTGGCGGCAAA
GGAGAAGAAGAAGAAGAAGAAGAGCCTGGTGAAGCGGCTGAAGGGCTCTCAAAGCACCAAGAAACAACCC
CCAGTCTCCTTGCTGCGTCCACTCTGTGAGACAATGACTACCCCGAAATGTCATCTGAGTGTTCTGATCT
TGTCACACTGCAGACTCCCTGATGCAGTTTGCCGAGACCTTTCCGAGGCCCTGAAGGTAGCTCCTGCCCT
AAGGGAGTTGGGCCTCCTCCAGAGCCGGCTCACCAACACAGGCCTGCGTTTACTGTGCGAAGGCCTGGCT
TGGCCCAAGTGCCAGGTGAAGACACTCAGGATGCAGCTGCCTGACCTCCAAGAGGTGATCAATTACTTGG
TCATCGTGCTCCAGCAGAGCCCAGTCCTGACCACTCTGGACCTCAGTGGCTGTCAGCTGCCTGGCGTCAT
TGTGGAACCTCTGTGTGCAGCCCTGAAGCACCCTAAATGCTCCCTAAAGACCCTCAGTCTGACCTCTGTG
GAGCTGAGTGAGAACTCACTGAGGGATCTTCAAGCTGTGAAGACATCAAAGCCAGATCTGTCCATCATAT
ATTCAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207117 representing BC031139
Red=Cloning site Green=Tags(s)

MLKSAGTNGPRVQGELRTLCRLAREGILDHHKAQFSEEDLEKLKLRGSQVQTIFLNKKEIPGVLKTEVTY
QFIDQSFQEFLAALSYLLEAERTPGTPAGGVQKLLNSDAELRGHLALTTRFLFGLLNTEGLRDIGNHFGC
VVPDHVKKDTLRWVQGQSHPKGPPVGAKKTAELEDIEDAEEEEEEEEDLNFGLELLYCLYETQEEDFVRQ
ALSSLPEIVLERVRLTRMDLEVLNYCVQCCPDGQALRLVSCGLVAAKEKKKKKKSLVKRLKGSQSTKKQP
PVSLLRPLCETMTTPKCHLSVLILSHCRLPDAVCRDLSEALKVAPALRELGLLQSRLTNTGLRLLCEGLA
WPKCQVKTLRMQLPDLQEVINYLVIVLQQSPVLTTLDLSGCQLPGVIVEPLCAALKHPKCSLKTLSLTSV
ELSENSLRDLQAVKTSKPDLSIIYSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC031139
ORF Size 1338 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC031139, AAH31139
RefSeq Size 3454 bp
RefSeq ORF 1340 bp
Locus ID 101613
MW 126.6 kDa
Gene Summary The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. [provided by RefSeq, Feb 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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