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Angpt1 (NM_009640) Mouse Tagged ORF Clone
CAT＃: MR207984
- TrueORF®
Angpt1 (Myc-DDK-tagged) - Mouse angiopoietin 1 (Angpt1)
ORF Plasmid: tGFP
"NM_009640" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110046O21Rik; an; Ang; Ang-1; Ang1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207984 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGTTTTCCTTTCCTTTGCATTCTTCGCTGCCATTCTGACTCACATAGGGTGCAGCAACCAGCGCC GAAATCCAGAAAACGGAGGGAGAAGATATAACCGGATTCAACATGGGCAATGTGCCTACACTTTCATTCT TCCAGAACACGACGGGAACTGCCGTGAGAGTGCGACAGAGCAGTACAACACCAACGCTCTGCAAAGGGAT GCTCCACACGTGGAGCCGGATTTCTCTTCCCAGAAACTTCAGCATCTGGAGCATGTGATGGAAAATTATA CTCAGTGGCTGCAAAAACTTGAGAATTACATTGTGGAAAATATGAAGTCGGAGATGGCCCAGATACAACA GAATGCTGTTCAAAACCACACGGCCACCATGCTTGAGATAGGAACCAGTCTCTTATCTCAGACTGCAGAG CAGACCCGAAAGCTGACAGATGTTGAGACCCAGGTACTAAATCAAACATCCCGACTTGAAATACAACTGC TAGAGAATTCATTATCAACATACAAGCTAGAGAAGCAACTTCTCCAACAGACAAATGAAATTCTGAAGAT TCACGAAAAAAACAGTTTACTAGAGCACAAAATCTTAGAAATGGAGGGAAAACACAAAGAAGAATTGGAC ACCTTGAAGGAGGAGAAAGAAAACCTTCAAGGCTTGGTTTCTCGTCAGACATTCATCATCCAGGAGTTGG AGAAGCAACTTAGTAGAGCTACCAACAACAACAGCATCCTGCAGAAGCAACAACTGGAGCTCATGGACAC AGTTCATAACCTTATCAGCCTTTGCACTAAAGAAGGTGTTTTGCTAAAGGGAGGAAAAAGAGAAGAAGAG AAACCATTTCGAGACTGTGCGGATGTATATCAAGCTGGTTTTAATAAAAGTGGAATCTACACTATTTATT TTAATAATATGCCAGAACCCAAAAAGGTATTTTGCAATATGGATGTGAATGGGGGAGGTTGGACAGTAAT ACAACACCGGGAAGATGGAAGCCTGGATTTCCAGAGGGGCTGGAAGGAGTATAAAATGGGTTTTGGGAAT CCCTCTGGTGAATATTGGCTTGGGAACGAGTTCATTTTTGCAATAACCAGTCAGAGGCAGTACATGCTGA GGATTGAGCTGATGGACTGGGAAGGGAACCGAGCCTACTCACAGTACGACAGATTCCACATAGGAAATGA AAAGCAGAACTATAGGTTATATTTAAAAGGTCACACAGGGACAGCAGGCAAACAGAGCAGCTTGATCTTA CACGGTGCCGATTTCAGCACGAAGGATGCTGATAACGACAACTGTATGTGCAAATGCGCTCTCATGCTAA CAGGAGGTTGGTGGTTCGATGCCTGTGGCCCTTCCAATCTAAATGGAATGTTCTACACTGCGGGACAAAA TCATGGAAAACTGAATGGGATAAAGTGGCACTACTTCAAAGGGCCCAGTTACTCCTTACGTTCCACCACC ATGATGATCCGGCCCTTGGACTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207984 protein sequence
Red=Cloning site Green=Tags(s) MTVFLSFAFFAAILTHIGCSNQRRNPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRD APHVEPDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAE QTRKLTDVETQVLNQTSRLEIQLLENSLSTYKLEKQLLQQTNEILKIHEKNSLLEHKILEMEGKHKEELD TLKEEKENLQGLVSRQTFIIQELEKQLSRATNNNSILQKQQLELMDTVHNLISLCTKEGVLLKGGKREEE KPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNGGGWTVIQHREDGSLDFQRGWKEYKMGFGN PSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSLIL HGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTT MMIRPLDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009640 |
| ORF Size | 1497 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009640.2 |
| RefSeq Size | 4316 bp |
| RefSeq ORF | 1497 bp |
| Locus ID | 11600 |
| UniProt ID | O08538 |
| MW | 57.5 kDa |
| Gene Summary | This gene encodes a secreted glycoprotein that belongs to the angiopoietin family of vascular growth factors. The encoded protein is a ligand in the vascular tyrosine kinase signaling pathway and regulates the formation and stabilization of blood vessels. This protein also functions in striated muscles by promoting proliferation, migration and differentiation of skeletal myoblasts and plays an essential role in the vascular response to tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216929 | Angpt1 (untagged) - Mouse angiopoietin 1 (Angpt1), (10ug) |
CNY 5,488.00 |
|
| MG207984 | Angpt1 (tGFP-tagged) - Mouse angiopoietin 1 (Angpt1) |
CNY 5,420.00 |
|
| MR207984L3 | Lenti ORF clone of Angpt1 (Myc-DDK-tagged) - Mouse angiopoietin 1 (Angpt1) |
CNY 7,888.00 |
|
| MR207984L4 | Lenti ORF clone of Angpt1 (mGFP-tagged) - Mouse angiopoietin 1 (Angpt1) |
CNY 7,888.00 |
