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Cyp1a2 (NM_009993) Mouse Tagged ORF Clone
CAT＃: MR208242
- TrueORF®
Cyp1a2 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2)
ORF Plasmid: tGFP
"NM_009993" in other vectors (4)
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CNY 3,832.00
CNY 4,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CP12; Cyp1a1; CYPIA2; P450-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208242 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCTCCCAGTACATCTCCTTAGCCCCAGAGCTGCTACTGGCCACTGCCATCTTCTGTTTAGTGT TCTGGATGGTCAGAGCCTCAAGGACCCAGGTTCCCAAAGGCCTGAAGAATCCACCCGGACCCTGGGGCTT GCCCTTCATTGGGCACATGCTGACTGTGGGGAAGAACCCACACCTGTCACTGACACGGCTGAGTCAGCAG TATGGGGACGTGCTGCAGATCCGCATCGGCTCCACTCCTGTGGTGGTGCTGAGCGGCCTGAACACCATCA AGCAGGCCCTGGTGAGGCAGGGAGATGACTTCAAGGGCCGACCAGACCTCTACAGCTTCACACTTATCAC TAACGGCAAGAGCATGACTTTCAACCCAGACTCTGGACCCGTGTGGGCTGCCCGCCGGCGCCTGGCCCAG GATGCCCTGAAGAGCTTCTCCATAGCCTCGGACCCGACGTCAGCATCCTCTTGCTATTTGGAGGAGCACG TGAGCAAGGAGGCTAACCATCTCGTCAGCAAGCTTCAGAAGGCGATGGCAGAGGTTGGCCACTTCGAACC AGTCAGCCAGGTGGTGGAATCGGTGGCTAACGTCATTGGTGCCATGTGCTTTGGGAAGAACTTCCCCCGG AAGAGCGAGGAGATGCTGAACATCGTGAATAACAGCAAGGACTTTGTGGAGAATGTCACCTCAGGGAATG CAGTGGACTTCTTCCCGGTCCTGCGCTACCTGCCCAACCCGGCCCTCAAGAGGTTTAAGACCTTCAATGA TAACTTCGTGCTGTTTCTGCAGAAAACTGTCCAGGAGCACTACCAAGACTTCAACAAGAACAGTATCCAA GACATCACAAGTGCCCTGTTCAAGCACAGCGAGAACTACAAAGACAATGGCGGTCTCATCCCCGAGGAGA AGATTGTCAACATTGTCAATGACATCTTTGGAGCTGGCTTTGACACAGTCACCACAGCCATCACCTGGAG CATTTTGCTACTTGTGACATGGCCTAACGTGCAGAGGAAGATCCATGAGGAGCTGGACACGGTGGTTGGC AGGGATCGGCAACCACGGCTTTCTGACCGTCCCCAGCTGCCATATCTAGAGGCCTTCATCCTGGAGATCT ACCGATACACATCCTTTGTCCCCTTCACCATCCCCCACAGCACAACGAGGGACACCTCACTGAATGGCTT CCACATTCCCAAGGAGCGCTGTATCTACATAAACCAGTGGCAGGTCAACCATGATGAGAAGCAGTGGAAA GACCCCTTTGTGTTCCGCCCAGAGCGGTTTCTTACCAATAACAACTCGGCCATCGACAAGACCCAGAGCG AGAAGGTGATGCTCTTCGGCTTGGGAAAGCGCCGGTGCATTGGGGAGATCCCGGCCAAGTGGGAAGTCTT CCTCTTCTTAGCCATCCTGCTGCAGCATCTGGAGTTTAGTGTGCCACCGGGTGTGAAGGTGGACCTGACA CCCAACTATGGGTTGACCATGAAGCCCGGGACCTGTGAACACGTCCAGGCATGGCCACGCTTTTCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208242 protein sequence
Red=Cloning site Green=Tags(s) MAFSQYISLAPELLLATAIFCLVFWMVRASRTQVPKGLKNPPGPWGLPFIGHMLTVGKNPHLSLTRLSQQ YGDVLQIRIGSTPVVVLSGLNTIKQALVRQGDDFKGRPDLYSFTLITNGKSMTFNPDSGPVWAARRRLAQ DALKSFSIASDPTSASSCYLEEHVSKEANHLVSKLQKAMAEVGHFEPVSQVVESVANVIGAMCFGKNFPR KSEEMLNIVNNSKDFVENVTSGNAVDFFPVLRYLPNPALKRFKTFNDNFVLFLQKTVQEHYQDFNKNSIQ DITSALFKHSENYKDNGGLIPEEKIVNIVNDIFGAGFDTVTTAITWSILLLVTWPNVQRKIHEELDTVVG RDRQPRLSDRPQLPYLEAFILEIYRYTSFVPFTIPHSTTRDTSLNGFHIPKERCIYINQWQVNHDEKQWK DPFVFRPERFLTNNNSAIDKTQSEKVMLFGLGKRRCIGEIPAKWEVFLFLAILLQHLEFSVPPGVKVDLT PNYGLTMKPGTCEHVQAWPRFSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009993 |
| ORF Size | 1542 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009993.3 |
| RefSeq Size | 1892 bp |
| RefSeq ORF | 1542 bp |
| Locus ID | 13077 |
| UniProt ID | P00186 |
| MW | 58.2 kDa |
| Gene Summary | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer. Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. Metabolizes caffeine via N3-demethylation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202251 | Cyp1a2 (untagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2), (10ug) |
CNY 2,000.00 |
|
| MG208242 | Cyp1a2 (tGFP-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) |
CNY 4,940.00 |
|
| MR208242L3 | Lenti ORF clone of Cyp1a2 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) |
CNY 6,370.00 |
|
| MR208242L4 | Lenti ORF clone of Cyp1a2 (mGFP-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 2 (Cyp1a2) |
CNY 6,370.00 |
