Tmem63b (BC057136) Mouse Tagged ORF Clone
CAT＃: MR208385
- TrueORF®
Tmem63b (Myc-DDK-tagged) - Mouse transmembrane protein 63b (cDNA clone MGC:62871 IMAGE:6529222)
ORF Plasmid: tGFP
"BC057136" in other vectors (4)
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CNY 3896.00
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC026370 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208385 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAACCCCAAACCGTGTGGTCACCTCTGCTGCTGTGTGGTTCGAGGCTGCGAGCAGGTGGAGGCCA TTGAGTACTACACAAAGCTGGAGCAGAGGCTGAAGGAAGACTACAGGCGGGAGAAGGAGAAAGTGAACGA GAAACCTCTGGGCATGGCCTTTGTCACCTTCCACAATGAGACCATCACAGCCATCATCCTAAAGGACTTC AACGTGTGCAAATGCCAGGGCTGTACCTGCCGCGGGGAGCCGAGAGCCTCCTCCTGCAGCGAAGCACTGC ATATCTCCAACTGGACCGTGACCTACGCCCCTGACCCTCAGAACATCTATTGGGAACACCTCTCCATCCG AGGCTTCATCTGGTGGCTGCGCTGCCTGGTCATCAATGTGGTCCTCTTCATTCTGCTCTTCTTCCTCACC ACCCCGGCCATCATCATCACCACCATGGACAAGTTCAACGTCACCAAGCCTGTGGAGTACCTCAACAACC CGATCATCACCCAGTTCTTCCCTACGCTACTGCTGTGGTGCTTCTCGGCCCTGCTCCCCACCATTGTCTA CTACTCCGCCTTCTTCGAAGCACACTGGACGCGCTCCGGGGAGAACAGGACGACCATGCACAAGTGCTAC ACCTTTCTCATCTTCATGGTGCTGCTTCTGCCCTCGCTGGGGCTGAGCAGCCTAGACCTCTTCTTCCGCT GGCTCTTTGACAAGAAATTCCTGGCTGAAGCAGCTATTCGGTTTGAGTGTGTGTTCCTGCCCGACAACGG CGCTTTCTTCGTGAACTACGTCATTGCCTCAGCCTTTATCGGTAACGCCATGGACCTGCTGCGCATCCCA GGCCTACTCATGTACATGATCCGGCTCTGCCTGGCGCGCTCCGCCGCTGAAAGACGCAACGTGAAGCGGC ATCAGGCCTACGAGTTCCAGTTTGGCGCAGCCTACGCTTGGATGATGTGCGTCTTCACGGTGGTCATGAC CTACAGTATCACCTGCCCCATCATCGTGCCCTTCGGGCTCATGTACATGCTGCTGAAGCACCTGGTAGAC AGGTACAATCTCTACTACGCCTACTTGCCGGCCAAACTGGACAAGAAGATCCACTCGGGGGCTGTGAACC AGGTGGTGGCTGCGCCCATCCTCTGCCTCTTCTGGTTGCTCTTCTTCTCCACCATGCGCACGGGTTTCCT AGCCCCCACATCTATGTTCACCTTCGTTGTTCTGGTAATCACCATCGTCATCTGTCTCTGCCACGTCTGC TTTGGACACTTCAAATACCTCAGTGCCCACAACTACAAGATTGAACACACAGAGACAGATGCCGTGAGCT CCAGAAGTAATGGACGGCCCCCCACTGCTGGCGCTGTCCCCAAATCTGCGAAATACATCGCTCAGGTGCT GCAGGACTCAGAGGGGGACGGGGACGGAGATGGGGCTCCTGGGAGCTCAGGCGATGAGCCCCCATCGTCC TCCTCCCAAGACGAGGAGTTGCTGATGCCTCCTGATGGCCTCACGGACACAGACTTCCAGTCATGCGAGG ACAGCCTCATAGAGAATGAGATTCACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208385 protein sequence
Red=Cloning site Green=Tags(s) MINPKPCGHLCCCVVRGCEQVEAIEYYTKLEQRLKEDYRREKEKVNEKPLGMAFVTFHNETITAIILKDF NVCKCQGCTCRGEPRASSCSEALHISNWTVTYAPDPQNIYWEHLSIRGFIWWLRCLVINVVLFILLFFLT TPAIIITTMDKFNVTKPVEYLNNPIITQFFPTLLLWCFSALLPTIVYYSAFFEAHWTRSGENRTTMHKCY TFLIFMVLLLPSLGLSSLDLFFRWLFDKKFLAEAAIRFECVFLPDNGAFFVNYVIASAFIGNAMDLLRIP GLLMYMIRLCLARSAAERRNVKRHQAYEFQFGAAYAWMMCVFTVVMTYSITCPIIVPFGLMYMLLKHLVD RYNLYYAYLPAKLDKKIHSGAVNQVVAAPILCLFWLLFFSTMRTGFLAPTSMFTFVVLVITIVICLCHVC FGHFKYLSAHNYKIEHTETDAVSSRSNGRPPTAGAVPKSAKYIAQVLQDSEGDGDGDGAPGSSGDEPPSS SSQDEELLMPPDGLTDTDFQSCEDSLIENEIHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057136 |
| ORF Size | 1569 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057136, AAH57136 |
| RefSeq Size | 1702 bp |
| RefSeq ORF | 1571 bp |
| Locus ID | 224807 |
| MW | 59.5 kDa |
| Gene Summary | Acts as an osmosensitive calcium-permeable cation channel.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217997 | Tmem63b (untagged) - Mouse transmembrane protein 63b (cDNA clone MGC:62871 IMAGE:6529222), (10ug) |
CNY 6940.00 |
|
| MG208385 | Tmem63b (tGFP-tagged) - Mouse transmembrane protein 63b (cDNA clone MGC:62871 IMAGE:6529222) |
CNY 3230.00 |
|
| MR208385L3 | Lenti ORF clone of Tmem63b (Myc-DDK-tagged) - Mouse transmembrane protein 63b (cDNA clone MGC:62871 IMAGE:6529222) |
CNY 4850.00 |
|
| MR208385L4 | Lenti ORF clone of Tmem63b (mGFP-tagged) - Mouse transmembrane protein 63b (cDNA clone MGC:62871 IMAGE:6529222) |
CNY 4850.00 |
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