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Cyp27a1 (NM_024264) Mouse Tagged ORF Clone
CAT＃: MR208548
- TrueORF®
Cyp27a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_024264" in other vectors (4)
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CNY 5,960.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300013A03Rik; Cyp27 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208548 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGTGGAGCCGCACGAGGCTGAGATGGACGCTCCTGGACCCGCGTGTGGTGGGCCGTGGCCTCT GCCCACAAGGGGCCAGAGCCAAGGCCACGATCCCTGCAGCCCTCCAGGCACAGGAGAGTACGGAGGGTCC AGGAACAGGTCAAGACCGGCCGCGCCTGCGGAGTCCGGCGGAGCTTCCGGGGACCGGAACGCTACAATTT TTATTCCAGCTATTTCTACAAGGCTATGTGCTGCACTTGCCCGACCTCCAGGTGCTGAACAAGACCAAGT ATGGTCCAATGTGGACAACCTCCTTTGGGACTTACACCAATGTGAATCTGGCTAGTGCCCCACTCTTGGA GCAAGTGATGAGACAGGAGGGCAAGTACCCAATAAGAGACCACATGGATCAGTGGAAGGACCACCGAGAC CACAAGGGCCTCACCTATGGGATCTTCATCGCACAAGGAGAGCAATGGTACCATCTGCGTCAGGCTTTGA AACAGAGGCTGCTGAAGCCTGACGAGGCCGCGCTCTACACGGATGCCTTAAACGAGGTTATCAGTGACTT TATCACCCGGCTGGACCAGGTGCGGGCAGAGAGTGAATCAGGGGACCAGGTGCCAGACATGGCTCATCTT CTCTACCACCTTGCCTTGGAAGCCATCACCTATATCCTGTTTGAGAAAAGGATTGGCTGCCTGAAACCCT CCATTCCTGAGGACACTGCCGCCTTCATCAGATCTGTTGCAATCATGTTCCAGAACTCAGTCTATATCAC TTTCCTTCCCAAATGGACGCGTCCTCTGCTGCCCTTTTGGAAGCGATACCTGAATGGCTGGGATAACATT TTCTCCTTTGGAAAGAAGCTGATTGATGAAAAAGTCCAGGAGCTAAAAGCCCAGCTACAGGAAACTGGGC CAGATGGAGTCCGGGTATCTGGCTACCTGCACTTCCTGCTGACCAATGAATTGCTCAGTACTCAGGAGAC CATCGGCACCTTTCCTGAGCTGCTTTTGGCTGGGGTGGACACGACATCCAACACACTGACATGGGCCCTG TACCACCTTTCAAAGAGCCCAGAGATCCAGGAGGCCTTGCACAAGGAAGTGACTGGTGTGGTGCCCTTCG GGAAGGTGCCCCAGCACAAGGACTTTGCCCACATGCCTCTGCTAAAAGCTGTGATTAAGGAGACCCTGCG CCTCTACCCTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTTCTC TTCCCCAAGAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGGGATCCCAGTGTCTTTCCTGAGC CCAACAGCTTCCAGCCTCACCGATGGCTGAGGAAGAAAGAGGCTGATAACCCTGGGATCCTACATCCATT CGGCTCTGTGCCCTTCGGCTATGGGGTTCGGTCTTGCCTGGGTCGGAGGATTGCAGAACTGGAGATGCAA CTGATGCTGTCAAGGCTGGTACAGAAGTATGAGATTGCCCTGGCTCCCGGGATGGGAGAAGTAAAGACTG TGTCCCGCATCGTCCTGGTTCCCAGCAAGAAGGTGAGGCTGCATTTTCTGCAGAGACAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR208548 protein sequence
Red=Cloning site Green=Tags(s) MAAWSRTRLRWTLLDPRVVGRGLCPQGARAKATIPAALQAQESTEGPGTGQDRPRLRSPAELPGTGTLQF LFQLFLQGYVLHLPDLQVLNKTKYGPMWTTSFGTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRD HKGLTYGIFIAQGEQWYHLRQALKQRLLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHL LYHLALEAITYILFEKRIGCLKPSIPEDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNI FSFGKKLIDEKVQELKAQLQETGPDGVRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDTTSNTLTWAL YHLSKSPEIQEALHKEVTGVVPFGKVPQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFL FPKNTQFVLCHYVVSRDPSVFPEPNSFQPHRWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQ LMLSRLVQKYEIALAPGMGEVKTVSRIVLVPSKKVRLHFLQRQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024264 |
| ORF Size | 1602 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024264.5 |
| RefSeq Size | 1890 bp |
| RefSeq ORF | 1602 bp |
| Locus ID | 104086 |
| UniProt ID | Q9DBG1 |
| MW | 60.7 kDa |
| Gene Summary | Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid. Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the "neutral" (classic) and the "acid" (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (By similarity). Plays a role in cholestanol metabolism in the cerebellum (PubMed:28190002). Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation. Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium. May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205465 | Cyp27a1 (untagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 2,000.00 |
|
| MG208548 | Cyp27a1 (tGFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1) |
CNY 7,560.00 |
|
| MR208548L3 | Lenti ORF clone of Cyp27a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein |
CNY 6,560.00 |
|
| MR208548L4 | Lenti ORF clone of Cyp27a1 (mGFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein |
CNY 6,560.00 |
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