Cyp27a1 (NM_024264) Mouse Untagged Clone
CAT＃: MC205465
Cyp27a1 (untagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein, (10ug)
CNY 3976.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1300013A03Rik; Cyp27 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC055028
CTGGATCTAAACTCTTGGCTTCTCGGGCACGATCCATGGCTGCGTGGAGCCGCACGAGGCTGAGATGGAC GCTCCTGGACCCGCGTGTGGTGGGCCGTGGCCTCTGCCCACAAGGGGCCAGAGCCAAGGCCACGATCCCT GCAGCCCTCCAGGCACAGGAGAGTACGGAGGGTCCAGGAACAGGTCAAGACCGGCCGCGCCTGCGGAGTC CGGCGGAGCTTCCGGGGACCGGAACGCTACAATTTTTATTCCAGCTATTTCTACAAGGCTATGTGCTGCA CTTGCCCGACCTCCAGGTGCTGAACAAGACCAAGTATGGTCCAATGTGGACAACCTCCTTTGGGACTTAC ACCAATGTGAATCTGGCTAGTGCCCCACTCTTGGAGCAAGTGATGAGACAGGAGGGCAAGTACCCAATAA GAGACCACATGGATCAGTGGAAGGACCACCGAGACCACAAGGGCCTCACCTATGGGATCTTCATCGCACA AGGAGAGCAATGGTACCATCTGCGTCAGGCTTTGAAACAGAGGCTGCTGAAGCCTGACGAGGCCGCGCTC TACACGGATGCCTTAAACGAGGTTATCAGTGACTTTATCACCCGGCTGGACCAGGTGCGGGCAGAGAGTG AATCAGGGGACCAGGTGCCAGACATGGCTCATCTTCTCTACCACCTTGCCTTGGAAGCCATCACCTATAT CCTGTTTGAGAAAAGGATTGGCTGCCTGAAACCCTCCATTCCTGAGGACACTGCCGCCTTCATCAGATCT GTTGCAATCATGTTCCAGAACTCAGTCTATATCACTTTCCTTCCCAAATGGACGCGTCCTCTGCTGCCCT TTTGGAAGCGATACCTGAATGGCTGGGATAACATTTTCTCCTTTGGAAAGAAGCTGATTGATGAAAAAGT CCAGGAGCTAAAAGCCCAGCTACAGGAAACTGGGCCAGATGGAGTCCGGGTATCTGGCTACCTGCACTTC CTGCTGACCAATGAATTGCTCAGTACTCAGGAGACCATCGGCACCTTTCCTGAGCTGCTTTTGGCTGGGG TGGACACGACATCCAACACACTGACATGGGCCCTGTACCACCTTTCAAAGAGCCCAGAGATCCAGGAGGC CTTGCACAAGGAAGTGACTGGTGTGGTGCCCTTCGGGAAGGTGCCCCAGCACAAGGACTTTGCCCACATG CCTCTGCTAAAAGCTGTGATTAAGGAGACCCTGCGCCTCTACCCTGTGGTTCCCACAAACTCCCGGATCA TCACAGAAAAGGAAACTGAAATTAATGGCTTTCTCTTCCCCAAGAATACACAGTTTGTGTTATGCCACTA CGTGGTGTCCCGGGATCCCAGTGTCTTTCCTGAGCCCAACAGCTTCCAGCCTCACCGATGGCTGAGGAAG AAAGAGGCTGATAACCCTGGGATCCTACATCCATTCGGCTCTGTGCCCTTCGGCTATGGGGTTCGGTCTT GCCTGGGTCGGAGGATTGCAGAACTGGAGATGCAACTGATGCTGTCAAGGCTGGTACAGAAGTATGAGAT TGCCCTGGCTCCCGGGATGGGAGAAGTAAAGACTGTGTCCCGCATCGTCCTGGTTCCCAGCAAGAAGGTG AGGCTGCATTTTCTGCAGAGACAGTAGTACCGAGCTGGGCTCCCGCCTTCATGGAGCTCGTCCAGAAGCC CTGGCACAGAAGTTCTTGGCCAGTCTCATGTCACATGTCACGATGTCAGATTCAACAGGAGAACTCTGTG CCCTTCCTATAGACACCAAATGTCTGGCACAATCTCTACTGAGCAGCACCCACTTAAGACAGCAGAGCAC CTTATAATAACAGTCCTTGGGTATGATTTAAAATAAAATTTAAAATTCAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_024264 |
| Insert Size | 1602 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC055028, AAH55028 |
| RefSeq Size | 1883 bp |
| RefSeq ORF | 1602 bp |
| Locus ID | 104086 |
| UniProt ID | Q9DBG1 |
| Gene Summary | Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid. Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the "neutral" (classic) and the "acid" (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (By similarity). Plays a role in cholestanol metabolism in the cerebellum (PubMed:28190002). Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation. Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium. May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208548 | Cyp27a1 (tGFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1) |
CNY 7560.00 |
|
| MR208548 | Cyp27a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein |
CNY 5960.00 |
|
| MR208548L3 | Lenti ORF clone of Cyp27a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein |
CNY 6560.00 |
|
| MR208548L4 | Lenti ORF clone of Cyp27a1 (mGFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (Cyp27a1), nuclear gene encoding mitochondrial protein |
CNY 6560.00 |
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