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Trim29 (NM_023655) Mouse Tagged ORF Clone
CAT＃: MR209159
- TrueORF®
Trim29 (Myc-DDK-tagged) - Mouse tripartite motif-containing 29 (Trim29)
"NM_023655" in other vectors (3)
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CNY 6,992.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110047J21Rik; 2810431N19Rik; 4732461M22Rik; AI119726 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209159 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGTGCCGATGCCTGCAGGAGCAATGGGGCCAGCCCGGAAGCCAGGGACACCCGCAGCCCACCGG GTCCCAGCGGCAGCCTAGAGAATGGCACTAAAGCAGACAGTAAGGACACCAAGACAACCAACGGGCACAG TGGGGAGGTGACAGAGGGCAAGACCCTGGGCAGTGCCCTGAAGTCAGGGGAGGGGAAGAGTGGCCTATTT TCCAGCAATGAGTGGCGCAGGCCTATCATCCAGTTTGTGGAGTCTGTCGACGACAAAGGCTCCAGTTACT TCAGTATGGACTCTGCGGAAGGCAGGAGGTCCCCTTATGCGGGGCTCCAGTTGGGGGCTTCCAAGAAACC TCCTGTGACCTTTGCTGAGAAGGGGGAGCTGCGAAAGTCCATTTTCTCCGAGCCCCGCAAACCCACGGTG ACCATCGTGGAGCCCGGGGAAGTCCGCAGGAATAGCTACCCCAGGGCTGACTCCAGCCTCCTGGCGCGGG CAAAGTCGGGCTCCGAGGAGGTGCTGTGCGACTCCTGCATCGGCAACAAGCAGAAGGCCGTCAAGTCCTG CCTGGTGTGCCAGGCTTCCTTCTGTGAGCTGCATCTGAAGCCCCACCTGGAGGGCGCTGCCTTCCGGGAC CACCAGCTGCTCGAGCCCATCCGAGACTTCGAGGCCCGAAAATGTCCCTTGCACGGCAAGACTATGGAGC TCTTCTGTCAGACCGACCAGACCTGCATCTGTTATCTCTGCATGTTCCAGGAGCACAAGAACCACAGCAC CGTGACGGTGGAGGAGGCCAAGGCAGAGAAGGAGACCGAGCTGTCACTGCAAAAGGAACAGCTGCAACTG AAGATCATTGAGATTGAAGATGATGTTGAGAAGTGGCAAAAGGAGAAAGACCGGATCAAGAGCTTTACCA CCAACGAGAAGGCGATCCTGGAGCAGAACTTCAGGGACCTGGTGAGGGAGCTGGAGAAGCAGAAGGAGGA AGTACGGGCTGCACTGGAGCAGCGGGAGCAGGATGCTGTGGACCAAGTGAAGGTGATCGTGGATGCTCTG GATGAGAGGGCCAAGGTGCTGCACGAGGACAAGCAGACCCGAGAGCAGCTGCACAACATCAGTGACTCGG TGCTGTTCCTGCAGGAATTTGGTGCATTGATGAGCAATTACTCTCTCCCTCCACCTCTGCCCACCTACCA TGTCCTGCTGGAGGGAGAGGGGCTGGGACAGTCTCTTGGCAACTGCAAGGATGACCTGCTCAATGTGTGC ATGCGTCATGTTGAGAAGATGTGCAAGGCCGATTTGAGCCGCAACTTCATTGAGAGGAACCATATGGAAA ATGGTGGGGACCATCGCTACATGAACAGCTACACCAGCAGCTATGGGAACGAGTGGAGCACGCCTGACAC CATGAAAAGATACTCCATGTACCTCACGCCCAAGGGTGGGGGCCGGACATCCTACCAGCCATCCTCGCCT AGCCGCCTCTCCAAGGAGACCAACCAGAAGAATTTTAACAATCTGTATGGCACAAAAGGCAACTATACCT CCAGGGTCTGGGAGTACACATCCACGGTTCAGAATTCTGAGGATATGCCCACGGTGCAAGGCAACTCCTC CTTCTCCCTGAAAGGCTTTCCCTCCCTCCTCCGGAGCCAAGTTCCCAAGGCCCAGCCTCAGACTTGGAAA TCTGGCAAGCAGACTCTGCTGTCTCACTACCGGCCATTCTATGTCAACAAGGGCAGCGGCATCGGATCCA ACGAGGCGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209159 protein sequence
Red=Cloning site Green=Tags(s) MEGADACRSNGASPEARDTRSPPGPSGSLENGTKADSKDTKTTNGHSGEVTEGKTLGSALKSGEGKSGLF SSNEWRRPIIQFVESVDDKGSSYFSMDSAEGRRSPYAGLQLGASKKPPVTFAEKGELRKSIFSEPRKPTV TIVEPGEVRRNSYPRADSSLLARAKSGSEEVLCDSCIGNKQKAVKSCLVCQASFCELHLKPHLEGAAFRD HQLLEPIRDFEARKCPLHGKTMELFCQTDQTCICYLCMFQEHKNHSTVTVEEAKAEKETELSLQKEQLQL KIIEIEDDVEKWQKEKDRIKSFTTNEKAILEQNFRDLVRELEKQKEEVRAALEQREQDAVDQVKVIVDAL DERAKVLHEDKQTREQLHNISDSVLFLQEFGALMSNYSLPPPLPTYHVLLEGEGLGQSLGNCKDDLLNVC MRHVEKMCKADLSRNFIERNHMENGGDHRYMNSYTSSYGNEWSTPDTMKRYSMYLTPKGGGRTSYQPSSP SRLSKETNQKNFNNLYGTKGNYTSRVWEYTSTVQNSEDMPTVQGNSSFSLKGFPSLLRSQVPKAQPQTWK SGKQTLLSHYRPFYVNKGSGIGSNEAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023655 |
| ORF Size | 1761 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023655.2, NP_076144.2 |
| RefSeq Size | 2830 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 72169 |
| UniProt ID | Q8R2Q0 |
| MW | 65.8 kDa |
| Gene Summary | Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of proinflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of TMEM173/STING in a similar way, leading to its degradation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TRIM29 negatively controls antiviral immune response through targeting STING for degradation
,Li, Q;Lin, L;Tong, Y;Liu, Y;Mou, J;Wang, X;Wang, X;Gong, Y;Zhao, Y;Liu, Y;Zhong, B;Dai, L;We, YQ;Zhang, H;Hu, H;,
Cell Discov
,PubMed ID 29581886
[TRIM29]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219424 | Trim29 (untagged) - Mouse tripartite motif-containing 29 (Trim29), (10ug) |
CNY 7,510.00 |
|
| MR209159L3 | Lenti ORF clone of Trim29 (Myc-DDK-tagged) - Mouse tripartite motif-containing 29 (Trim29) |
CNY 7,790.00 |
|
| MR209159L4 | Lenti ORF clone of Trim29 (mGFP-tagged) - Mouse tripartite motif-containing 29 (Trim29) |
CNY 7,790.00 |
