Tmem2 (BC019745) Mouse Tagged ORF Clone
CAT＃: MR210404
- TrueORF®
Tmem2 (Myc-DDK-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225)
ORF Plasmid: tGFP
"BC019745" in other vectors (3)
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CNY 5432.00
Product images
CNY 300.00
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210404 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTGATGGAGTGTTTGGAAACTACGTCCCTGTGCCTACCACAGATTGCATGGCTGTTTCAACTTTCT GGATTGCCCACCCCAACAACCACCTGATCAATAACGCAGCTGCAGGCTCACAGGATGCTGGAATATGGTA TTTATTCCACAAGGAACCAACTGGGGAGTCCAGTGGGCTGCAGCTCCTGGAGAAGCCAGAACTCACTCCG CTGGGGATATTTTATAACAACAGGGTTCATTCAAATTTTAAGGCTGGTTTATTTGTGGACAAAGGTGTCA AAACGACCAACGCCAGTGCATCTGACCCAAGGGAGTACCTCTGCCTGGATAACAGTGCAAGGTTTCGGCC CCATCAGGATGCAGACCCTGAGAAGCCACGCGTTGCCGCCATCATTGACAGGCTCATTGCTTTTAAGAAC AATGACAATGGAGCCTGGGTCCGAGGAGGAGATATTATTGTTCAGAATTCAGCATTTGCAGATAACGGAA AGGGGCTGACCTTTGCCAGTGATGGGAGCTTCCCAAGCGATGAAGGGTCCAGTCAGGAGGTGACGGAGTC CCTCTTTGTCGGGGAGAGTAGGAATTATGGCTTTCAAGGTGGCCAAAACAAGTACATGGGCACAGGGGGA ATCGATCAGAAGCCTCGGACGCTACCCAGGAACAGGACATTCCCAATCCGAGGCTTTCAGATTTACGATG GGCCTATTCATCTCACAAAGAGTACTTTCAAAAAGTATGTGCCAACTCCAGACAGGTACAGCAGTGCAAT TGGCTTCCTCATGAAGAATTCCTGGCAGACAACACCCAGGAATAACGTTTCCCTTGTGAAGTTTGGTCCA CAGGTCTCTCTGAACGTCTTCTTTGGAAAGCCTGGCCCCTGGTTTGAGGATTGTGAGCTGGACGGCGATA AGAACTCCATCTTCCATGACATTGATGGCTCTGTGACAGGATACAAGGACACCTATGTGGGAAGGATGGA CAACTACCTGATCCGCCATCCAAACTGTGTCAATGTTACTAAGTGGAATGCAGTGATCTGTAGCGGGACC TACGCCCAGGTGTATGTTCAGACCTGGAACACTCCGAATCTTAGCATGATCATCACACGGGATGAATACC CATCCCACCCAATGGTGCTTCGGGGTATCAACCAGAGGGCCATCTCTCCACAGTACCAGCCTGTCGTCAT GCTGGAGAAGGGCTACACCATCCACTGGAATGGTCCAGCGCCACGAACCACTTTCTTGTACCTCGTGAAC TTCAACAAGGATGACTGGATCCGGGTTGGCCTCTGTTACCCAGCCAACACGAGCTTTCAGGTTACCGTTG GCTTTCTGCAGCGGCAGAACGGCTCATTATCTAGAATTGAGGACTATGAGCCTGCGCGGTCGATGGAGGA ACTGCAGAAGAAGCCGTCTGAGAGAAAATTCTATTTTGACTCTGGCACAGGGTTATTGTTTCTGTATCTC AGAGCCCACAGCCACAGAGATGGCCACAGTTACTGTTCATCTCAGGGATGTGAAAGAGTCAAGATTCAGG CAGCAACAGACTCCAAAGATATCAGTAACTGCATGGCCAAAGCTTACCCGCAGTATTACAAGAAGCCCTC GGCGGTCAAGCGCATGCCGGCCATGCTCACGGGGCTCTGTCAAGGCTGTGGAACCCACCAGATGGTGTTT ACCAGTGACCCTCACAAAAGCTACCTCCCTGTACGATTCCAGTCACCTGGGAAAGCAGAAATTCAGCGAG GAGATCCATCCATTATTTCCGTCAATGGTACTGACTTTACCTTCCGGAGTGCGGGTGTCCTCCTCCTCGT TGTGGATGCCTGCAGTGTGCCTTTCCGGGTGAAGGAAAAGCGGATGTTCCTTTCTGCAGACGTCAGTCAC ATGGAAGAATATTTCAAGGCTAGCATCCCCCCAAGGTCAATCGTTCTGTTGAGCACAAGAGGAGAAATTA AGCAGCTGAATATCTCGGATTCATTAGCACTTCTGGGATTGGCCAAGCCAGCACATCTGTACAGCAAAGG TAGTGTCGTATTTTTGGGATTCAGCGGAAACTTCGCACCGTCATGGACGAAGCTATTCACCAGTCCCAAT GAGCAGGGCCTCGGAGTGCTTGAGCAGTTTCTCCCGTTGCAGATGGAGGAATATGGTTGCTCCCGGACCG GCAGTGTCCACAGAAGAGACCTTGACCTGTTACAGCAGGCTTTGAAAGTGCTT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >MR210404 protein sequence
Red=Cloning site Green=Tags(s) MRDGVFGNYVPVPTTDCMAVSTFWIAHPNNHLINNAAAGSQDAGIWYLFHKEPTGESSGLQLLEKPELTP LGIFYNNRVHSNFKAGLFVDKGVKTTNASASDPREYLCLDNSARFRPHQDADPEKPRVAAIIDRLIAFKN NDNGAWVRGGDIIVQNSAFADNGKGLTFASDGSFPSDEGSSQEVTESLFVGESRNYGFQGGQNKYMGTGG IDQKPRTLPRNRTFPIRGFQIYDGPIHLTKSTFKKYVPTPDRYSSAIGFLMKNSWQTTPRNNVSLVKFGP QVSLNVFFGKPGPWFEDCELDGDKNSIFHDIDGSVTGYKDTYVGRMDNYLIRHPNCVNVTKWNAVICSGT YAQVYVQTWNTPNLSMIITRDEYPSHPMVLRGINQRAISPQYQPVVMLEKGYTIHWNGPAPRTTFLYLVN FNKDDWIRVGLCYPANTSFQVTVGFLQRQNGSLSRIEDYEPARSMEELQKKPSERKFYFDSGTGLLFLYL RAHSHRDGHSYCSSQGCERVKIQAATDSKDISNCMAKAYPQYYKKPSAVKRMPAMLTGLCQGCGTHQMVF TSDPHKSYLPVRFQSPGKAEIQRGDPSIISVNGTDFTFRSAGVLLLVVDACSVPFRVKEKRMFLSADVSH MEEYFKASIPPRSIVLLSTRGEIKQLNISDSLALLGLAKPAHLYSKGSVVFLGFSGNFAPSWTKLFTSPN EQGLGVLEQFLPLQMEEYGCSRTGSVHRRDLDLLQQALKVL TRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-NotI Cloning Scheme for this gene |
| ACCN | BC019745 |
| ORF Size | 2223 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC019745, AAH19745 |
| RefSeq Size | 2374 bp |
| RefSeq ORF | 2225 bp |
| Locus ID | 83921 |
| MW | 82.8 kDa |
| Gene Summary | Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 5 kDa fragments (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity). Is very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210404 | Tmem2 (tGFP-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225) |
CNY 4560.00 |
|
| MR210404L3 | Lenti ORF clone of Tmem2 (Myc-DDK-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225) |
CNY 6080.00 |
|
| MR210404L4 | Lenti ORF clone of Tmem2 (mGFP-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225) |
CNY 6080.00 |
