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Lrrc8c (NM_133897) Mouse Tagged ORF Clone
CAT＃: MR210745
- TrueORF®
Lrrc8c (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8 family, member C (Lrrc8c)
ORF Plasmid: tGFP
"NM_133897" in other vectors (4)
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CNY 5,800.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AD158; AI326115; E430036I04Rik; fad158 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210745 representing NM_133897
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCGGTGACCGAGTTCCGGCAGTTCTCCGAGCAGCAGCCCGCCTTCCGGGTGCTGAAGCCGTGGT GGGATGTGTTCACGGATTACCTCTCGGTGGCCATGCTGATGATCGGAGTGTTTGGATGTACTTTACAGGT CATGCAAGACAAGATCATCTGCCTTCCAAAAAGAGTGCAGCCTGCTCAGAACCACTCTTCCGTTCCCAAT GTCTCCCAGGCAGTTATCAGTACCACTCCGCTGCCCCCACCTAAACCCTCACCGACCAACCCCGCGACCG TGGAGATGAAGGGACTGAAGACAGACCTGGACCTTCAGCAGTACAGTTTCATCAACCAGATGTGCTACGA GCGAGCCCTCCACTGGTATGCCAAGTACTTCCCATACCTGGTGCTCATCCACACCCTGGTCTTCATGCTC TGTAGCAACTTCTGGTTCAAATTCCCCGGATCCAGTTCCAAAATAGAACATTTCATCTCCATCCTGGGGA AGTGCTTTGACTCCCCGTGGACCACACGGGCTCTCTCCGAAGTGTCCGGAGAGGACTCCGAAGAGAAGGA CAACAGAAAAAACAACATGAACAGGTCCGGCACCATCCAGTCTGGTCCCGAGGGCAACCTGGTGAGGTCC CAGTCTCTCAAGTCAATTCCTGAAAAGTTCGTGGTTGACAAATCTGCTGCGGGGGCTCTGGACAAGAAGG AAGGTGAGCAGGCCAAGGCCTTGTTTGAGAAGGTGAAGAAGTTCAGACTGCATGTGGAAGAAGGTGATAT CCTGTACGCCATGTACGTGCGACAGACTGTGCTGAAGGTCATCAAATTCCTAATCATCATTGCCTACAAC AGTGCCCTGGTTTCCAAAGTCCAGTTCACAGTGGACTGTAATGTGGACATCCAGGACATGACGGGCTATA AGAACTTTTCTTGCAATCACACCATGGCTCATTTGTTCTCTAAGCTCTCCTTTTGCTACCTGTGCTTTGT AAGCATCTATGGCCTGACGTGCCTTTACACCTTATACTGGCTGTTCTACCGTTCTCTGAGGGAATATTCG TTCGAGTATGTCCGGCAGGAGACGGGAATCGATGACATTCCGGATGTGAAAAATGACTTTGCTTTCATGC TCCATATGATAGACCAGTATGACCCTCTCTATTCCAAGCGGTTTGCAGTGTTCCTGTCTGAAGTCAGCGA GAACAAGTTAAAACAACTCAATTTAAATAACGAGTGGACCCCGGACAAACTGCGGCAGAAGCTGCAGACA AATGCCCACAACCGCCTGGAGCTGCCGCTGATCATGCTGTCTGGCCTCCCGGACACCGTGTTTGAAATCA CAGAGTTGCAGTCTCTGAAGCTGGAGATCATTAAGAATGTCATGATCCCCGCCACCATCGCCCAGCTAGA CAACCTGCAGGAGCTCTGTCTGCACCAGTGCTCCGTCAAGATCCACAGCGCCGCGCTCTCCTTCCTGAAG GAGAATCTCAAGGTCCTGAGTGTCAAGTTTGATGACATGAGGGAGCTGCCCCCCTGGATGTACGGCCTCC GGAACCTAGAAGAGCTCTATCTGGTTGGCTCTCTGAGTCACGACATCTCCAAAAATGTCACCCTGGAGTC TCTGAGGGACCTCAAAAGCCTTAAAATCCTTTCCATCAAGAGCAACGTCTCCAAGATCCCTCAGGCCGTG GTGGATGTGTCCAGCCACCTCCAGAAGATGTGCGTCCACAATGATGGCACCAAACTGGTGATGCTCAACA ACCTGAAGAAGATGACCAACCTGACGGAGCTGGAGCTGGTCCACTGCGACCTGGAACGCATCCCCCACGC CGTGTTCAGCCTGCTCAGTCTCCAGGAGCTGGACCTGAAGGAGAACAACCTCAAGTCCATAGAGGAGATC GTGAGCTTCCAGCACTTGAGAAAGCTGACCGTGCTCAAACTGTGGTATAACAGCATCGCTTACATTCCAG AGCACATCAAGAAACTGACCAGCCTGGAGAGACTGTTTTTCAGCCACAATAAGGTAGAGGTGCTTCCCTC CCACCTCTTCCTGTGCAACAAAATCAGATACCTGGACTTGTCCTATAACGACATCCGCTTCATCCCGCCC GAAATCGGAGTTCTGCAAAGTTTACAGTATTTTTCCATCACTTGTAACAAAGTGGAGAGCCTCCCAGATG AACTCTACTTCTGCAAGAAACTTAAAACTTTGAAGATTGGGAAAAACAGCCTCTCCGTACTTTCACCAAA AATTGGAAATCTACTATTTCTTTCCTACTTAGACATCAAAGGCAATCACTTTGAAGTCCTCCCTCCCGAG CTGGGAGACTGCCGGGCTCTGAAGCGAGCCAGGCTAGTTGTGGAAGATGCTCTGTTTGAGACTCTGCCCT CAGATGTCCGGGAGCAGATGAAAGCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210745 representing NM_133897
Red=Cloning site Green=Tags(s) MIPVTEFRQFSEQQPAFRVLKPWWDVFTDYLSVAMLMIGVFGCTLQVMQDKIICLPKRVQPAQNHSSVPN VSQAVISTTPLPPPKPSPTNPATVEMKGLKTDLDLQQYSFINQMCYERALHWYAKYFPYLVLIHTLVFML CSNFWFKFPGSSSKIEHFISILGKCFDSPWTTRALSEVSGEDSEEKDNRKNNMNRSGTIQSGPEGNLVRS QSLKSIPEKFVVDKSAAGALDKKEGEQAKALFEKVKKFRLHVEEGDILYAMYVRQTVLKVIKFLIIIAYN SALVSKVQFTVDCNVDIQDMTGYKNFSCNHTMAHLFSKLSFCYLCFVSIYGLTCLYTLYWLFYRSLREYS FEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSENKLKQLNLNNEWTPDKLRQKLQT NAHNRLELPLIMLSGLPDTVFEITELQSLKLEIIKNVMIPATIAQLDNLQELCLHQCSVKIHSAALSFLK ENLKVLSVKFDDMRELPPWMYGLRNLEELYLVGSLSHDISKNVTLESLRDLKSLKILSIKSNVSKIPQAV VDVSSHLQKMCVHNDGTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEI VSFQHLRKLTVLKLWYNSIAYIPEHIKKLTSLERLFFSHNKVEVLPSHLFLCNKIRYLDLSYNDIRFIPP EIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNLLFLSYLDIKGNHFEVLPPE LGDCRALKRARLVVEDALFETLPSDVREQMKAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133897 |
| ORF Size | 2409 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133897.1, NM_133897.2, NP_598658.1 |
| RefSeq Size | 2829 bp |
| RefSeq ORF | 2412 bp |
| Locus ID | 100604 |
| UniProt ID | Q8R502 |
| MW | 92.8 kDa |
| Gene Summary | Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:29769723). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Plays a redundant role in the efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (By similarity). May play a role in adipogenesis (PubMed:15564382, PubMed:15184384, PubMed:21804215).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Structure of a volume-regulated anion channel of the LRRC8 family
,Deneka, D;Sawicka, M;Lam, AKM;Paulino, C;Dutzler, R;,
Nature
,PubMed ID 29769723
[LRRC8C]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201792 | Lrrc8c (untagged) - Mouse leucine rich repeat containing 8 family, member C (Lrrc8c), (10ug) |
CNY 2,000.00 |
|
| MG210745 | Lrrc8c (tGFP-tagged) - Mouse leucine rich repeat containing 8 family, member C (Lrrc8c) |
CNY 6,370.00 |
|
| MR210745L3 | Lenti ORF clone of Lrrc8c (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8 family, member C (Lrrc8c) |
CNY 7,700.00 |
|
| MR210745L4 | Lenti ORF clone of Lrrc8c (mGFP-tagged) - Mouse leucine rich repeat containing 8 family, member C (Lrrc8c) |
CNY 7,700.00 |
