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Lepr (BC082551) Mouse Tagged ORF Clone
CAT＃: MR210800
- TrueORF®
Lepr (Myc-DDK-tagged) - Mouse leptin receptor (cDNA clone MGC:105189 IMAGE:30621706)
ORF Plasmid: tGFP
"BC082551" in other vectors (4)
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CNY 8,984.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Obr, LEPROT, OB-RGRP, diabetes, obese-like |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210800 representing BC082551
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGTCAGAAATTCTATGTGGTTTTGTTACACTGGGAATTTCTTTATGTGATAGCTGCACTTAACC TGGCATATCCAATCTCTCCCTGGAAATTTAAGTTGTTTTGTGGACCACCGAACACAACCGATGACTCCTT TCTCTCACCTGCTGGAGCCCCAAACAATGCCTCGGCTTTGAAGGGGGCTTCTGAAGCAATTGTTGAAGCT AAATTTAATTCAAGTGGTATCTACGTTCCTGAGTTATCCAAAACAGTCTTCCACTGTTGCTTTGGGAATG AGCAAGGTCAAAACTGCTCTGCACTCACAGACAACACTGAAGGGAAGACACTGGCTTCAGTAGTGAAGGC TTCAGTTTTTCGCCAGCTAGGTGTAAACTGGGACATAGAGTGCTGGATGAAAGGGGACTTGACATTATTC ATCTGTCATATGGAGCCATTACCTAAGAACCCCTTCAAGAATTATGACTCTAAGGTCCATCTTTTATATG ATCTGCCTGAAGTCATAGATGATTCGCCTCTGCCCCCACTGAAAGACAGCTTTCAGACTGTCCAATGCAA CTGCAGTCTTCGGGGATGTGAATGTCATGTGCCGGTACCCAGAGCCAAACTCAACTACGCTCTTCTGATG TATTTGGAAATCACATCTGCCGGTGTGAGTTTTCAGTCACCTCTGATGTCACTGCAGCCCATGCTTGTTG TGAAACCCGATCCACCCTTAGGTTTGCATATGGAAGTCACAGATGATGGTAATTTAAAGATTTCTTGGGA CAGCCAAACAATGGCACCATCTCCGCTTCAATATCAGGTGAAATATTTAGAGAATTCTACAATTGTAAGA GAGGCTGCTGAAATTGTCTCAGCTACATCTCTGCTGGTAGACAGTGTGCTTCCTGGATCTTCATATGAGG TCCAGGTGAGGAGCAAGAGACTGGATGGTTCAGGAGTCTGGAGTGACTGGAGTTCACCTCAAGTCTTTAC CACACAAGATGTTGTGTATTTTCCACCCAAAATTCTGACTAGTGTTGGATCGAATGCTTCTTTTCATTGC ATCTACAAAAACGAAAACCAGATTATCTCCTCAAAACAGATAGTTTGGTGGAGGAATCTAGCTGAGAAAA TCCCTGAGATACAGTACAGCATTGTGAGTGACCGAGTTAGCAAAGTTACCTTCTCCAACCTGAAAGCCAC CAGACCTCGAGGGAAGTTTACCTATGACGCAGTGTACTGCTGCAATGAGCAGGCGTGCCATCACCGCTAT GCTGAATTATACGTGATCGATGTCAATATCAATATATCATGTGAAACTGACGGGTACTTAACTAAAATGA CTTGCAGATGGTCACCCAGCACAATCCAATCACTAGTGGGAAGCACTGTGCAGCTGAGGTATCACAGGCG CAGCCTGTATTGTCCTGATAGTCCATCTATTCATCCTACGTCTGAGCCCAAAAACTGCGTCTTACAGAGA GACGGCTTTTATGAATGTGTTTTCCAGCCAATCTTTCTATTATCTGGCTATACAATGTGGATCAGGATCA ACCATTCTTTAGGTTCACTTGACTCGCCACCAACGTGTGTCCTTCCTGACTCCGTAGTAAAACCACTACC TCCATCTAACGTAAAAGCAGAGATTACTGTAAACACTGGATTATTGAAAGTATCTTGGGAAAAGCCAGTC TTTCCGGAGAATAACCTTCAATTCCAGATTCGATATGGCTTAAGTGGAAAAGAAATACAATGGAAGACAC ATGAGGTATTCGATGCAAAGTCAAAGTCTGCCAGCCTGCTGGTGTCAGACCTCTGTGCAGTCTATGTGGT CCAGGTTCGCTGCCGGCGGTTGGATGGACTAGGATATTGGAGTAATTGGAGCAGTCCAGCCTATACGCTT GTCATGGATGTAAAAGTTCCTATGAGAGGGCCTGAATTTTGGAGAAAAATGGATGGGGACGTTACCTTCT CATGGCCCATGAGTAAAGTGAGTGCTGTGGAGTCACTCAGTGCTTATCCCCTGAGCAGCAGCTGTGTCAT CCTTTCCTGGACACTGTCACCTGATGATTATAGTCTGTTATATCTGGTTATTGAATGGAAGATCCTTAAT GAAGATGATGGAATGAAGTGGCTTAGAATTCCCTCGAATGTTAAAAAGTTTTATATCCACGATAATTTTA TTCCCATCGAGAAATATCAGTTTAGTCTTTACCCAGTATTTATGGAAGGAGTTGGAAAACCAAAGATAAT TAATGGTTTCACCAAAGATGCTATCGACAAGCAGCAGAATGACGCAGGGCTGTATGTCATTGTACCCATA ATTATTTCCTCTTGTGTCCTACTGCTCGGAACACTGTTAATTTCACACCAGAGAATGAAAAAGTTGTTTT GGGACGATGTTCCAAACCCCAAGAATTGTTCCTGGGCACAAGGACTGAATTTCCAAAAGAGAACGGACAC TCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210800 representing BC082551
Red=Cloning site Green=Tags(s) MMCQKFYVVLLHWEFLYVIAALNLAYPISPWKFKLFCGPPNTTDDSFLSPAGAPNNASALKGASEAIVEA KFNSSGIYVPELSKTVFHCCFGNEQGQNCSALTDNTEGKTLASVVKASVFRQLGVNWDIECWMKGDLTLF ICHMEPLPKNPFKNYDSKVHLLYDLPEVIDDSPLPPLKDSFQTVQCNCSLRGCECHVPVPRAKLNYALLM YLEITSAGVSFQSPLMSLQPMLVVKPDPPLGLHMEVTDDGNLKISWDSQTMAPSPLQYQVKYLENSTIVR EAAEIVSATSLLVDSVLPGSSYEVQVRSKRLDGSGVWSDWSSPQVFTTQDVVYFPPKILTSVGSNASFHC IYKNENQIISSKQIVWWRNLAEKIPEIQYSIVSDRVSKVTFSNLKATRPRGKFTYDAVYCCNEQACHHRY AELYVIDVNINISCETDGYLTKMTCRWSPSTIQSLVGSTVQLRYHRRSLYCPDSPSIHPTSEPKNCVLQR DGFYECVFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSNVKAEITVNTGLLKVSWEKPV FPENNLQFQIRYGLSGKEIQWKTHEVFDAKSKSASLLVSDLCAVYVVQVRCRRLDGLGYWSNWSSPAYTL VMDVKVPMRGPEFWRKMDGDVTFSWPMSKVSAVESLSAYPLSSSCVILSWTLSPDDYSLLYLVIEWKILN EDDGMKWLRIPSNVKKFYIHDNFIPIEKYQFSLYPVFMEGVGKPKIINGFTKDAIDKQQNDAGLYVIVPI IISSCVLLLGTLLISHQRMKKLFWDDVPNPKNCSWAQGLNFQKRTDTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC082551 |
| ORF Size | 2454 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC082551.1 |
| RefSeq Size | 4812 bp |
| RefSeq ORF | 2456 bp |
| Locus ID | 16847 |
| MW | 176.4 kDa |
| Gene Summary | Receptor for hormone LEP/leptin (Probable) (PubMed:11861497). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS (PubMed:10799542, PubMed:25383904, PubMed:11923481, PubMed:11861497). In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (PubMed:10660043, PubMed:12594516). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed:25383904, PubMed:11923481). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis (PubMed:12594516). Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus (PubMed:25383904). Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production (PubMed:9732873).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220733 | Lepr (untagged) - Mouse leptin receptor (cDNA clone MGC:105189 IMAGE:30621706), (10ug) |
CNY 9,120.00 |
|
| MG210800 | Lepr (tGFP-tagged) - Mouse leptin receptor (cDNA clone MGC:105189 IMAGE:30621706) |
CNY 10,584.00 |
|
| MR210800L3 | Lenti ORF clone of Lepr (Myc-DDK-tagged) - Mouse leptin receptor (cDNA clone MGC:105189 IMAGE:30621706) |
CNY 10,070.00 |
|
| MR210800L4 | Lenti ORF clone of Lepr (mGFP-tagged) - Mouse leptin receptor (cDNA clone MGC:105189 IMAGE:30621706) |
CNY 10,070.00 |
