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Trim28 (NM_011588) Mouse Tagged ORF Clone
CAT＃: MR210883
- TrueORF®
Trim28 (Myc-DDK-tagged) - Mouse tripartite motif-containing 28 (Trim28)
ORF Plasmid: tGFP
"NM_011588" in other vectors (4)
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CNY 6,112.00
CNY 8,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA408787; KAP-1; KRIP-1; MommeD9; Tif1b; Tif1beta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210883 representing NM_011588
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCTCGGCGGCAGCGACTGCAGCGGCCTCGGCCGCGACGGCCGCCTCGGCGGCCTCTGGTAGCC CAGGGTCGGGCGAGGGCTCGGCGGGCGGTGAGAAGCGTCCGGCTGCTTCCTCAGCCGCGGCGGCCTCTGC AGCCGCGTCGTCCCCTGCGGGGGGCGGTGGCGAGGCGCAGGAGCTTCTGGAGCACTGCGGCGTGTGTCGC GAGCGCCTGCGGCCCGAGCGGGATCCTCGGCTGCTGCCCTGTCTACATTCGGCCTGCAGTGCCTGCCTGG GCCCCGCTACACCCGCCGCAGCGAATAATTCGGGGGATGGCGGCTCGGCGGGCGACGGCGCTATGGTGGA TTGTCCAGTGTGCAAACAGCAGTGCTACTCCAAAGACATCGTGGAGAATTATTTTATGCGTGATAGTGGC AGTAAGGCCTCTTCTGATTCCCAGGATGCTAACCAGTGCTGCACTAGCTGTGAAGATAATGCCCCAGCCA CTAGCTATTGTGTGGAGTGCTCTGAACCACTTTGTGAGACCTGTGTGGAGGCTCACCAGCGGGTGAAATA CACCAAGGACCACACTGTGCGCTCCACAGGACCTGCTAAGACTCGAGATGGAGAGCGAACAGTCTACTGT AATGTGCACAAGCATGAGCCCCTCGTGCTGTTCTGTGAGAGCTGTGACACACTCACCTGCCGCGACTGCC AGCTCAACGCTCACAAGGACCATCAGTACCAGTTTTTGGAAGATGCAGTGAGGAACCAACGTAAACTCTT GGCTTCACTGGTGAAACGTCTTGGGGACAAACATGCCACACTTCAGAAAAACACCAAGGAGGTTCGAAGC TCGATCCGCCAGGTGTCTGATGTGCAGAAGCGAGTGCAGGTTGATGTCAAGATGGCCATTCTGCAGATCA TGAAGGAGCTGAATAAGCGGGGTCGAGTTCTGGTCAATGATGCCCAGAAGGTGACCGAGGGTCAGCAGGA ACGTCTGGAGCGCCAGCACTGGACCATGACCAAAATTCAGAAGCACCAGGAACACATTTTGCGTTTTGCC TCTTGGGCTCTGGAGAGTGATAACAATACAGCTCTCTTGCTCTCTAAGAAGCTGATCTATTTCCAGCTGC ATCGGGCCCTCAAAATGATTGTGGATCCTGTGGAGCCTCATGGTGAGATGAAGTTTCAGTGGGATCTCAA TGCCTGGACCAAGAGTGCTGAAGCCTTTGGCAAGATTGTGGCTGAGCGTCCTGGTACGAACTCCACAGGT CCTGGGCCCATGGCTCCTCCAAGAGCCCCAGGCCCTCTAAGCAAGCAAGGTTCTGGCAGTAGCCAGCCCA TGGAAGTACAAGAGGGATATGGCTTTGGGTCAGATGATCCCTATTCAAGTGCAGAGCCGCATGTATCAGG CATGAAGCGGTCCCGCTCTGGTGAGGGAGAGGTAAGTGGCCTCTTAAGGAAGGTGCCACGTGTGAGCCTT GAACGCCTGGATCTGGACCTCACCTCTGACAGCCAGCCACCAGTCTTCAAGGTCTTTCCTGGAAGCACTA CTGAGGACTACAATCTGATTGTTATTGAGCGTGGTGCTGCTGCAGCAGCTGCTGGTCAGGCTGGGACTGT GCCACCAGGAGCCCCTGGTGCCCCACCCCTTCCTGGCATGGCCATTGTCAAGGAAGAAGAGACAGAAGCT GCTATTGGAGCTCCCCCGGCTGCCCCCGAGGGTCCTGAAACCAAGCCTGTGTTGATGCCTCTGACTGAAG GTCCTGGTGCCGAGGGACCTCGTCTAGCTTCACCTAGTGGCAGTACCAGCTCAGGCTTGGAGGTGGTGGC TCCTGAGGTTACCTCAGCCCCAGTAAGTGGGCCAGGTATCCTGGATGACAGTGCCACTATCTGCCGTGTC TGCCAGAAACCAGGTGACCTGGTCATGTGTAACCAGTGCGAATTTTGCTTCCACCTGGATTGCCACCTCC CTGCCCTGCAGGATGTTCCAGGGGAGGAATGGAGTTGCTCACTCTGCCACGTGCTCCCTGACCTAAAGGA GGAAGATGGAAGCCTCAGCCTGGATGGAGCAGATAGCACTGGTGTGGTAGCTAAACTCTCACCAGCCAAC CAGCGGAAATGTGAGCGTGTTCTCCTGGCCCTGTTCTGCCATGAACCATGCCGTCCCTTGCATCAGCTGG CTACCGACTCTACATTCTCCATGGAGCAGCCTGGTGGTACCCTAGACCTGACCTTGATTCGTGCTCGCCT CCAAGAGAAGCTGTCACCTCCTTATAGCTCCCCCCAGGAGTTTGCTCAAGATGTGGGCCGCATGTTCAAA CAGTTCAACAAGCTGACTGAGGACAAGGCAGATGTTCAGTCCATCATCGGCTTGCAGCGCTTCTTTGAGA CACGCATGAATGATGCCTTTGGTGACACCAAGTTTTCTGCTGTGCTGGTAGAACCACCACCATTGAACCT TCCCAGTGCTGGCCTAAGTTCTCAGGAGCTCTCTGGCCCTGGTGATGGCCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210883 representing NM_011588
Red=Cloning site Green=Tags(s) MAASAAATAAASAATAASAASGSPGSGEGSAGGEKRPAASSAAAASAAASSPAGGGGEAQELLEHCGVCR ERLRPERDPRLLPCLHSACSACLGPATPAAANNSGDGGSAGDGAMVDCPVCKQQCYSKDIVENYFMRDSG SKASSDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKTRDGERTVYC NVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRS SIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFA SWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTG PGPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGMKRSRSGEGEVSGLLRKVPRVSL ERLDLDLTSDSQPPVFKVFPGSTTEDYNLIVIERGAAAAAAGQAGTVPPGAPGAPPLPGMAIVKEEETEA AIGAPPAAPEGPETKPVLMPLTEGPGAEGPRLASPSGSTSSGLEVVAPEVTSAPVSGPGILDDSATICRV CQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPAN QRKCERVLLALFCHEPCRPLHQLATDSTFSMEQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFK QFNKLTEDKADVQSIIGLQRFFETRMNDAFGDTKFSAVLVEPPPLNLPSAGLSSQELSGPGDGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011588 |
| ORF Size | 2502 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011588.3, NP_035718.2 |
| RefSeq Size | 3255 bp |
| RefSeq ORF | 2505 bp |
| Locus ID | 21849 |
| UniProt ID | Q62318 |
| MW | 89.3 kDa |
| Gene Summary | Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (PubMed:20164836). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (By similarity). Acts as a corepressor for ZFP568 (PubMed:22110054, PubMed:27658112).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203421 | Trim28 (untagged) - Mouse tripartite motif-containing 28 (Trim28), (10ug) |
CNY 2,000.00 |
|
| MG210883 | Trim28 (tGFP-tagged) - Mouse tripartite motif protein 28 (Trim28) |
CNY 9,220.00 |
|
| MR210883L3 | Lenti ORF clone of Trim28 (Myc-DDK-tagged) - Mouse tripartite motif-containing 28 (Trim28) |
CNY 10,260.00 |
|
| MR210883L4 | Lenti ORF clone of Trim28 (mGFP-tagged) - Mouse tripartite motif-containing 28 (Trim28) |
CNY 8,512.00 |
