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Zfp281 (NM_177643) Mouse Tagged ORF Clone
CAT＃: MR211084
- TrueORF®
Zfp281 (Myc-DDK-tagged) - Mouse zinc finger protein 281 (Zfp281), transcript variant 2
ORF Plasmid: tGFP
"NM_177643" in other vectors (3)
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CNY 6,544.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Znf281 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211084 representing NM_177643
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATCGGCAGCGGGTTCCTTAGCGGCGGCGGCGGTCCCAGCAGTAGCGGTGGTAGCGGCTCCGGCG GCAGCTCCGGCAGCGCCAGCGGCGGCAGCGGCGGCGGCAGGAGAGCAGAGATGGAGCCCACCTTCCCCCA GAGTATGGTTATGTTCAACCACCGGCTTCCCCCGGTCACCAGCTTCACCCGGCCGGCGGGGACGGCCGCC CCTCCCCCGCAGTGCGTGTTATCCTCCTCTACCTCCGCAGCCCCGGCCGCTGAGCCCCCCCCTCCGCCAG CCCCGGACATGACTTTCAAGAAGGAGCCGGCGGCGTCAGCCGCGGCCTTCCCTTCGCAGAGGACCTCCTG GGGATTCTTGCAGTCCTTGGTGAGCATCAAGCAGGAGAAACCTGCGGATCCCGAGGAGCAGCCGTCCCAC CACCACCATCACCACCACCACTATGGGGGGCTGTTCGCTGGGGCTGAAGAGCGATCACCAGGCCTAGGAG GAGGGGAAGGGGGGAGCCACGGCGTCATCCAAGACCTCAGTCTTCTGCACCAGCATGCCCAGCAGCAACC GGCCCAGCACCACCGCGATGTATTACTGAGCAGCGGTAGCAGGACTGATGAGCACGGCAACCAGGAGCCA AAGCAGGACGCTAACGTCAAAAAGGCAAAGAGGCCAAAGCCAGAATCTCAGGGAATCAAAGCCAAGAGGA AGCCAAGTGCATCTTCCAAACCTTTGGTTGGAGAGGGAGAAGGTGCCGTCCTGTCCCCAAGTCAGAAACC TCATATCTGTGATCACTGTAGTGCTGCTTTCAGGAGCTCCTACCACCTGCGGAGACATGTCCTCATCCAC ACGGGCGAAAGGCCTTTCCAGTGCAGCCAGTGTAGTATGGGTTTCATCCAGAAATACCTACTGCAGAGAC ACGAGAAAATTCACAGCAGAGAGAAACCCTTTGGGTGCGATCAGTGCAGCATGAAGTTTATTCAGAAGTA CCATATGGAGAGACACAAGAGGACACATAGTGGAGAAAAGCCATACAAGTGTGACACTTGCCAACAGTAT TTTTCAAGGACTGACAGATTGTTGAAGCACAGGCGCACGTGTGGTGAAGCCATAGCAAAAGGAGCCGCTA GTGCAGAACCTGGGTCATCAAACCATAACAGTATGGGTAACCTGGCTGTGTTGTCTCAGGGAAATACAAG TTCCTCAAGGAGAAAGTCGAAGTCGAAAAGCATAGCTATTGAAAATAAGGAACACAAGACTGGCAAAACA AATGAATCACAAATGTCAAACAATATAAACATGCAGAGTTATTCAGTAGAAATGCCTACTGTGTCTACCA GTGGGAGCATAATTGGCACTGGAATAGATGAACTGCAGAAAAGGGTGCCAAAATTGATCTTTAAGAAAGG AAGCAGAAAGAATGCAGATAAAAGCTACCTTAATTTTGTGTCACCGTTACCAGACGTAGTTGGGCAGAAA TCCTTGTCTGGTAAGCCAGGTGGCTCACTTGGCATCGTATCGAATAATAGTGTGGAGACCATTAGTCTTC TCCAAAGTACAAGTGGCAAGCAAGGCCCGATAAGTAGTAATTATGATGATGCCATGCAGTTTTCAAAGAA GAGAAGATACCTACCAACTGCCAGCAGCAACAGTGCCTTTTCTATCAATGTAGGACACATGGTCTCCCAG CAGTCAGTCATTCAGTCTGCAGGGGTCAGTGTTTTGGACAATGAGGCGCCATTGTCACTTATTGACTCCT CAGCTCTAAATGCTGAAATTAAGTCTTGTCACGACAAGTCTGGAATTCCTGATGAGGTTTTACAAAGTAT TTTGGATCAGTACTCTGGCAAATCAGAAACACAGAAGGAGGATCCTTTCAATTTAACAGAACCACGAGTG GATTTACACACCTCAGGAGAACACTCTGAATTGGTTCAAGAAGAAAATTTGAGCCCAGGCACCCAAACAC CTTCAAATGATAAAACAAGCATGTTGCAAGAATACTCCAAATACCTCCAACAGGCTTTCGAAAAATCCAC TAATGCAGGTTTTACTCTTGGACACGGTTTCCAATTTGTCAGCTTGTCTTCACCTCTCCACAACCACACT TTGTTTCCAGAAAAACAGATATACACTACATCTCCTTTGGAGTGTGGTTTTGGCCAGTCTGTTACCTCAG TGCTGCCATCTTCGTTGCCAAAGCCTCCTTTTGGGATGTTGTTTGGATCTCAACCAGGTCTTTATTTATC TGCTTTGGATGCTACACATCAGCAGTTGACACCTTCCCAGGAGCTGGATGATCTGATAGATTCTCAGAAG AACTTAGAGACATCATCAGCCTTCCAGTCCTCATCTCAGAAATTGACTAGCCAGAAGGAACAACAGAAAA ATTTAGAGTCCTCAACGAGCTTTCAGATTCCATCTCAGGAGTTAGCTAGCCAGATTGATCCTCAGAAAGA CATAGAGCCTAGAACAACGTACCAGATTGAGAACTTTGCACAAGCATTCGGTTCTCAGTTTAAGTCGGGC AGCAGGGTGCCAATGACCTTTATCACTAACTCTAATGGAGAAGTGGACCATAGAGTAAGGACTTCAGTGT CAGATTTCTCAGGGTATACAAATATGATGTCTGATGTAAGTGAGCCATGTAGTACAAGAGTAAAGACACC CACCAGCCAGAGTTACAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211084 representing NM_177643
Red=Cloning site Green=Tags(s) MKIGSGFLSGGGGPSSSGGSGSGGSSGSASGGSGGGRRAEMEPTFPQSMVMFNHRLPPVTSFTRPAGTAA PPPQCVLSSSTSAAPAAEPPPPPAPDMTFKKEPAASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQPSH HHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQDLSLLHQHAQQQPAQHHRDVLLSSGSRTDEHGNQEP KQDANVKKAKRPKPESQGIKAKRKPSASSKPLVGEGEGAVLSPSQKPHICDHCSAAFRSSYHLRRHVLIH TGERPFQCSQCSMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTCQQY FSRTDRLLKHRRTCGEAIAKGAASAEPGSSNHNSMGNLAVLSQGNTSSSRRKSKSKSIAIENKEHKTGKT NESQMSNNINMQSYSVEMPTVSTSGSIIGTGIDELQKRVPKLIFKKGSRKNADKSYLNFVSPLPDVVGQK SLSGKPGGSLGIVSNNSVETISLLQSTSGKQGPISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHMVSQ QSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSCHDKSGIPDEVLQSILDQYSGKSETQKEDPFNLTEPRV DLHTSGEHSELVQEENLSPGTQTPSNDKTSMLQEYSKYLQQAFEKSTNAGFTLGHGFQFVSLSSPLHNHT LFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQK NLETSSAFQSSSQKLTSQKEQQKNLESSTSFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSG SRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177643 |
| ORF Size | 2679 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177643.4, NP_808311.1 |
| RefSeq Size | 5271 bp |
| RefSeq ORF | 2682 bp |
| Locus ID | 226442 |
| UniProt ID | Q99LI5 |
| MW | 96.7 kDa |
| Gene Summary | Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211084 | Zfp281 (tGFP-tagged) - Mouse zinc finger protein 281 (Zfp281) |
CNY 7,890.00 |
|
| MR211084L3 | Lenti ORF clone of Zfp281 (Myc-DDK-tagged) - Mouse zinc finger protein 281 (Zfp281), transcript variant 2 |
CNY 9,030.00 |
|
| MR211084L4 | Lenti ORF clone of Zfp281 (mGFP-tagged) - Mouse zinc finger protein 281 (Zfp281), transcript variant 2 |
CNY 9,030.00 |
