Nfatc4 (NM_023699) Mouse Tagged ORF Clone

CAT＃: MR211104

  • TrueORF®

Nfatc4 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1

Datasheet
SDS
Plasmid Map

  "NM_023699" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 6,600.00

CNY 7,890.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 3110041H08Rik; AW107667; AW546455; Nfat3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211104 representing NM_023699
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCCGCAAGCTGCGAGGATGAGGAGCTGGAATTTAAGCTGGTGTTCGGAGAGGAAAAGGAGCCCC
CCCCGCTGGGCCCTGGGGGTCCTGGGGAAGAGCTGGACTCAGAGGATACCCCTCCATGCTGTCGTTTGGC
CCTGGGGGAACCCCTTCCTTATGGTGCTGCCCCTATTGGGATTCCCCGACCCCCACCCCCTCGGCCAGGG
ATGCACTCACCACCGCCCCGTCCCGCCCCTTCACCTGGCACCTGGGAGAGCCAGCCAGCTAGGTCGGTGA
GGCTGGGGGGCCCAGGAGGTAATGCAGGGGGTGCTGGGGGTGGTCGTGTTCTTGAGTGTCCCAGCATCCG
GATTACCTCCATCTCTCCCACGCCTGACCCGCCGACCTCGCTAGAGGACACATCTGAAACTTGGGGGGAC
GGCTCTCCTAGAGATTACCCTCCACCAGAAGGCTTTGGGGGCTATCGAGAGGCTGGAGGTCAGGGCGGAG
GCGCTTTCTTCAGCCCGAGCCCTGGCAGCAGCAGCCTGTCTTCGTGGAGCTTCTTCTCGGACGCCTCCGA
CGAGGCAGCCCTGTATGCAGCGTGCGACGAGGTGGAGTCTGAACTTAATGAAGCAGCCTCCCGTTTTGGC
TTGAGCTCTCCGCTGCCCTCGCCCCGGGCTTCTCCTAGGCCATGGACCCCGGAGGATCCCTGGAGCCTAT
ACGGTCCAAGCTCGGGAGGCCGAGCGCCAGAGGATAGCTGGCTACTCCTCAGCGCTCCTGGGCCCGTCCC
AGCCTCCCCTCGGCCTGCTTCTCCCTGCGGCAAGCGGCGCTATTCCAGTTCCGGAACCCCATCTTCGGCC
TCCCCAGCTTTGTCCCGCCGAGGCAGCCTTGGGGAAGAAGGTCCAGAGCCACCTCCACCACCCCCGTTGC
CTCTGGTCCGGGACCCTAGTTCCCCTGGCCCTTTTGACTATGTGGGTGCTCCACCAACCGAGAGCATCCC
TCAGAAAACTCGGAGGACTTCTAGCGAGCAAGCAGTGGCCCTGCCTCGGTCTGAAGAGCCTCCCTCGTGC
AATGGGAAGCTACCCTCCGGTACAGAGGACTCTGTGGCTGCTCCGGGGGCTCTGCGGAAAGAGGTGGCCG
GCATGGATTACCTAGCAGTGCCTTCTCCCCTTGCTTGGTCCAAGGCCCGGATTGGGGGACACAGCCCCAT
CTTCAGGACCTCTGCCCTACCTCCCCTGGACTGGCCTTTACCCAGCCAGTATGAGCAGCTGGAGCTGAGG
ATCGAGGTACAGCCTAGAGCACACCACCGAGCTCACTATGAGACTGAGGGCAGCCGGGGGGCTGTCAAGG
CTGCTCCTGGAGGCCACCCTGTGGTCAAGCTCCTAGGCTACAGTGAGAAGCCATTGACTCTGCAGATGTT
CATTGGCACTGCAGATGAGAGGAGCCTGCGGCCCCATGCCTTCTACCAGGTGCACCGGATTACTGGCAAG
ATGGTGGCTACAGCCAGCTATGAAGCTGTAGTCAGTGGTACCAAAGTGTTGGAGATGACCTTACTCCCAG
AGAACAACATGGCTGCCAACATTGACTGTGCTGGAATCCTGAAGCTTCGAAATTCAGACATTGAGCTACG
GAAGGGTGAGACAGACATCGGGCGCAAAAACACGCGTGTGCGGCTGGTGTTCCGTGTGCACGTGCCTCAG
GGCGGCGGGAAGGTCGTGTCTGTGCAGGCAGCATCAGTGCCCATCGAGTGCTCCCAGCGCTCGGCCCAGG
AGCTGCCCCAGGTGGAGACCTACAGCCCCAGTGCCTGCTCTGTGAGAGGAGGGGAGGAGCTAGTGTTGAC
TGGTTCCAACTTCCTGCCAGACTCTAAAGTGGTGTTCATTGAGAGGGGTCCTGATGGAAAACTGCAGTGG
GAGGAAGAGGCAGCGGTGAACCGACTGCAGAGCAGTGAGGTGACATTGACTCTCACCATCCCCGAGTACA
GCAACAAGCGGGTGTCCCGACCAGTCCAGGTCTACTTTTACGTCTCCAATGGACGGAGGAAGCGCAGTCC
TACCCAAAGTTTCAAGTTCCTACCTGTGGTCTTCAAGGAGGAGCCCCTACCAGACTCATCTCTCCGGGGC
TTCCCTTCCACATCGGGTCCCCCCTTTGGCCCTGACGTGGACTTCTCACCGCCCAGGCCCCCTTACCCCT
CCTATCCCCATGAAGACCCTGCTTATGAGACTCCTTATCTGTCAGAAGGCTTTGGTTATAGCACACCCGC
TCTGTACCCCCAGACGGGGCCCCCACCGTCCTATAGATCTGGCCTGCGGATGTTCCCTGAGACTGGGGGT
ACCACAGGTTGTGCCCGCCTACCTTCAGTCTCTTTCCTTCCGCGCCCCTTTCCTGGTGATCAATATGGAG
GACAGGGCTCCTCTTTTGCCCTGGGGCTTCCATTCTCCCCTCCAGCCCCCTTTAGGCCGCCACTGCCTTC
CTCCCCACCACTCGAAGACCCCTTCCATCCCCAGAGTGCCATCCACCCCTTACCTCCTGAGGGCTACAAT
GAGGTGGGGCCAGGCTATACCCCTGGGGAGGGGGCTTCGGAGCAGGAGAAAGCCAGGGGTGGCTACAGCA
GCGGCTTCAGAGACAGTGTACCTATCCAGGGTATCACCCTGGAGGAAGTGAGTGAGATCATTGGCCGAGA
CCTGAGTGGCTTCCCTGCACGTCCTGGAGAAGAGCCTCCTGCC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
>MR211104 representing NM_023699
Red=Cloning site Green=Tags(s)

MGAASCEDEELEFKLVFGEEKEPPPLGPGGPGEELDSEDTPPCCRLALGEPLPYGAAPIGIPRPPPPRPG
MHSPPPRPAPSPGTWESQPARSVRLGGPGGNAGGAGGGRVLECPSIRITSISPTPDPPTSLEDTSETWGD
GSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFG
LSSPLPSPRASPRPWTPEDPWSLYGPSSGGRAPEDSWLLLSAPGPVPASPRPASPCGKRRYSSSGTPSSA
SPALSRRGSLGEEGPEPPPPPPLPLVRDPSSPGPFDYVGAPPTESIPQKTRRTSSEQAVALPRSEEPPSC
NGKLPSGTEDSVAAPGALRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELR
IEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERSLRPHAFYQVHRITGK
MVATASYEAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQ
GGGKVVSVQAASVPIECSQRSAQELPQVETYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQW
EEEAAVNRLQSSEVTLTLTIPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFKFLPVVFKEEPLPDSSLRG
FPSTSGPPFGPDVDFSPPRPPYPSYPHEDPAYETPYLSEGFGYSTPALYPQTGPPPSYRSGLRMFPETGG
TTGCARLPSVSFLPRPFPGDQYGGQGSSFALGLPFSPPAPFRPPLPSSPPLEDPFHPQSAIHPLPPEGYN
EVGPGYTPGEGASEQEKARGGYSSGFRDSVPIQGITLEEVSEIIGRDLSGFPARPGEEPPA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_023699
ORF Size 2703 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_023699.2
RefSeq Size 3284 bp
RefSeq ORF 2706 bp
Locus ID 73181
UniProt ID Q8K120
MW 95.8 kDa
Gene Summary Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed:17198697). Along with NFATC3, involved in embryonic heart development (PubMed:12750314, PubMed:17198697). Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function (PubMed:12750314). Transactivates many genes involved in heart physiology. Along with GATA4, binds to and activates NPPB/BNP promoter (PubMed:9568714). Activates NPPA/ANP/ANF and MYH7/beta-MHC transcription (By similarity). Binds to and transactivates AGTR2 gene promoter (PubMed:17198697). Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation (PubMed:22586092). In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during a developmental critical period when auditory neurons depend on afferent input for survival (PubMed:18354019). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP). Plays a role in adipocyte differentiation. May be involved in myoblast differentiation into myotubes (By similarity). Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription. Binds to PPARG gene promoter and regulates its activity (By similarity). Binds to PPARG and REG3G gene promoters (PubMed:17198697).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Ppt1-deficiency dysregulates lysosomal Ca ++ homeostasis contributing to pathogenesis in a mouse model of CLN1 disease. ,null, Journal of inherited metabolic disease ,PubMed ID 35150145 [Nfatc4]

Other Versions

SKU Description Size Price
MC204560 Nfatc4 (untagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1, (10ug)
    • 10 ug

CNY 2,000.00

MR211104L3 Lenti ORF clone of Nfatc4 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1
    • 10 ug

CNY 9,790.00

MR211104L4 Lenti ORF clone of Nfatc4 (mGFP-tagged) - Mouse nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (Nfatc4), transcript variant 1
    • 10 ug

CNY 9,790.00

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