Prdm16 (NM_001177995) Mouse Tagged Lenti ORF Clone
CAT#: MR211758L4
- LentiORF®
Lenti ORF clone of Prdm16 (mGFP-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 2
"NM_001177995" in other vectors (3)
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CNY 10,640.00
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | 5730557K01Rik; csp1; mel1 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR211758).
|
Restriction Sites | SgfI-NotI Cloning Scheme for this gene |
ACCN | NM_001177995 |
ORF Size | 3531 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177995.1, NP_001171466.1 |
RefSeq Size | 8433 bp |
RefSeq ORF | 3534 bp |
Locus ID | 70673 |
Gene Summary | Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223034 | Prdm16 (untagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 2, (10ug) |
CNY 12,920.00 |
|
MR211758 | Prdm16 (Myc-DDK-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 2 |
CNY 8,192.00 |
|
MR211758L3 | Lenti ORF clone of Prdm16 (Myc-DDK-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 2 |
CNY 10,640.00 |