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Hbegf (NM_010415) Mouse Tagged ORF Clone
CAT＃: MR212137
- TrueORF®
Hbegf (Myc-DDK-tagged) - Mouse heparin-binding EGF-like growth factor (Hbegf)
"NM_010415" in other vectors (5)
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CNY 3,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW047313; Dtr; Dts; Hegfl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212137 representing NM_010415
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGCTGCCGTCGGTGATGCTGAAGCTCTTTCTGGCCGCAGTGTTGTCCGCGTTGGTGACCGGTG AGAGTCTGGAGCGGCTTCGGAGAGGTCTGGCGGCAGCAACCAGCAACCCTGACCCTCCCACTGGATCCAC AAACCAGCTGCTACCCACGGGAGGTGATCGTGCTCAGGGGGTCCAGGACTTGGAAGGGACAGATCTGAAC CTTTTCAAAGTTGCTTTCTCCTCCAAGCCACAAGGCCTGGCCACCCCAAGCAAAGAAAGGAATGGGAAAA AGAAGAAGAAAGGAAAGGGGTTAGGGAAGAAGAGAGACCCATGCCTCAGGAAATACAAGGACTACTGCAT CCACGGGGAGTGCAGATACCTGCAGGAGTTCCGTACTCCCTCTTGCAAATGCCTCCCTGGTTACCACGGA CACAGGTGTCATGGGCTGACTCTACCAGTGGAGAATCCCCTATACACATATGACCACACTACAGTCTTGG CTGTGGTGGCTGTAGTACTGTCGTCCGTCTGTCTTCTTGTCATCGTGGGACTTCTCATGTTTAGGTACCA CAGGAGAGGAGGTTATGACTTGGAAAGTGAAGAGAAAGTGAAGTTGGGCGTGGCTAGCTCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212137 representing NM_010415
Red=Cloning site Green=Tags(s) MKLLPSVMLKLFLAAVLSALVTGESLERLRRGLAAATSNPDPPTGSTNQLLPTGGDRAQGVQDLEGTDLN LFKVAFSSKPQGLATPSKERNGKKKKKGKGLGKKRDPCLRKYKDYCIHGECRYLQEFRTPSCKCLPGYHG HRCHGLTLPVENPLYTYDHTTVLAVVAVVLSSVCLLVIVGLLMFRYHRRGGYDLESEEKVKLGVASSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010415 |
| ORF Size | 624 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010415.2, NP_034545.1 |
| RefSeq Size | 2337 bp |
| RefSeq ORF | 627 bp |
| Locus ID | 15200 |
| UniProt ID | Q06186 |
| MW | 23.3 kDa |
| Gene Summary | Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208020 | Hbegf (untagged) - Mouse heparin-binding EGF-like growth factor (Hbegf), (10ug) |
CNY 3,600.00 |
|
| MR212137L1 | Lenti ORF clone of Hbegf (Myc-DDK-tagged) - Mouse heparin-binding EGF-like growth factor (Hbegf) |
CNY 6,000.00 |
|
| MR212137L2 | Lenti ORF clone of Hbegf (mGFP-tagged) - Mouse heparin-binding EGF-like growth factor (Hbegf) |
CNY 5,890.00 |
|
| MR212137L3 | Lenti ORF clone of Hbegf (Myc-DDK-tagged) - Mouse heparin-binding EGF-like growth factor (Hbegf) |
CNY 6,000.00 |
|
| MR212137L4 | Lenti ORF clone of Hbegf (mGFP-tagged) - Mouse heparin-binding EGF-like growth factor (Hbegf) |
CNY 6,000.00 |
