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Grik2 (NM_001111268) Mouse Tagged ORF Clone
CAT＃: MR219234
- TrueORF®
Grik2 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), transcript variant 1
ORF Plasmid: tGFP
"NM_001111268" in other vectors (4)
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CNY 9,976.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW124492; C130030K03Rik; Glu; GluK2; Glur; Glur-6; Glur6; Glurbe; Glurbeta2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219234 representing NM_001111268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATTATTTCCCCAGTTTTAAGTAATCTAGTCTTCAGTCGCTCCATTAAAGTCCTGCTCTGCTTGT TGTGGATCGGATATTCGCAAGGAACCACACATGTGTTAAGATTCGGTGGTATATTTGAATATGTGGAATC TGGCCCTATGGGAGCTGAAGAACTTGCATTCAGATTTGCTGTGAATACAATCAACAGGAACAGGACTCTG CTACCCAATACCACGTTAACATATGATACACAGAAGATCAATCTCTATGACAGTTTTGAAGCATCTAAGA AAGCTTGTGATCAGCTGTCTCTTGGGGTGGCTGCCATCTTCGGTCCTTCACACAGTTCATCAGCAAATGC TGTTCAGTCCATCTGCAATGCTCTGGGGGTTCCTCACATACAGACCCGCTGGAAGCACCAGGTGTCAGAC AATAAGGATTCCTTCTATGTCAGTCTCTACCCAGACTTCTCTTCCCTCAGCCGTGCCATCTTGGATTTGG TGCAGTTTTTTAAGTGGAAAACTGTCACAGTTGTGTATGACGACAGCACTGGTCTCATTCGCTTGCAAGA GCTCATCAAAGCTCCATCAAGGTACAATCTTCGACTTAAAATTCGTCAGCTGCCAGCTGATACAAAAGAT GCAAAGCCTTTGCTGAAAGAGATGAAGAGGGGCAAGGAGTTCCACGTGATCTTCGACTGCAGCCATGAAA TGGCAGCAGGCATTTTAAAGCAGGCATTAGCTATGGGAATGATGACAGAATACTACCACTATATATTTAC GACTCTGGACCTCTTCGCTCTTGATGTGGAGCCCTACAGATACAGTGGCGTAAATATGACAGGGTTCAGA ATACTAAATACAGAGAATACCCAAGTCTCCTCCATCATCGAGAAGTGGTCGATGGAACGGTTACAGGCAC CTCCAAAACCTGACTCAGGTTTGCTGGATGGATTTATGACGACTGATGCTGCTCTGATGTATGATGCAGT GCACGTTGTGTCTGTAGCTGTCCAACAGTTTCCCCAGATGACAGTCAGCTCCTTGCAATGCAATCGACAC AAACCCTGGCGCTTTGGGACTCGCTTCATGAGCCTAATTAAAGAGGCTCATTGGGAAGGTCTTACAGGCA GAATTACATTTAACAAAACCAATGGATTGCGAACAGATTTTGATTTGGATGTGATCAGTCTCAAGGAAGA AGGTCTGGAGAAGATTGGGACTTGGGATCCATCCAGTGGCCTGAATATGACAGAAAGTCAGAAAGGGAAG CCAGCAAATATTACAGATTCATTGTCTAATCGTTCTTTGATTGTTACCACCATTTTGGAAGAACCATATG TCCTGTTTAAGAAGTCTGACAAACCTCTCTATGGGAATGATCGATTTGAAGGCTACTGTATTGATCTTCT ACGAGAGTTATCTACAATCCTTGGCTTTACATATGAAATTAGGCTTGTGGAGGATGGGAAATATGGAGCC CAGGATGATGTGAATGGACAATGGAATGGAATGGTTCGTGAGCTAATTGATCATAAAGCTGACCTTGCAG TTGCTCCACTGGCTATTACCTATGTTCGTGAGAAGGTCATCGACTTTTCAAAGCCGTTTATGACTCTTGG AATAAGTATTTTGTACCGCAAGCCCAATGGTACAAACCCAGGCGTCTTCTCCTTCCTGAATCCTCTCTCC CCTGATATCTGGATGTATGTTCTGCTGGCTTGCTTGGGTGTCAGTTGTGTGCTCTTTGTCATAGCCAGGT TTAGTCCCTATGAGTGGTATAATCCACACCCTTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTAC CTTGCTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCGGCAAGGTTCTGAGCTCATGCCCAAAGCA CTCTCCACCAGGATAGTGGGAGGCATTTGGTGGTTTTTCACACTTATCATCATTTCTTCGTATACCGCTA ACCTAGCCGCCTTTCTGACCGTGGAACGCATGGAGTCGCCTATTGACTCTGCTGACGATTTAGCTAAGCA AACCAAGATAGAATATGGAGCAGTAGAGGACGGCGCAACCATGACGTTTTTCAAGAAATCAAAAATCTCA ACGTATGATAAAATGTGGGCATTTATGAGCAGCAGGAGACAGTCTGTGCTTGTCAAAAGCAATGAGGAAG GGATTCAACGTGTCCTCACCTCCGATTATGCTTTCTTAATGGAGTCAACGACCATCGAGTTTGTTACCCA GCGGAACTGTAACCTCACGCAGATTGGTGGCCTTATAGACTCCAAAGGCTATGGTGTTGGCACTCCCATG GGTTCTCCATATCGAGACAAAATCACCATAGCCATTCTTCAGCTGCAGGAGGAAGGCAAGCTGCACATGA TGAAGGAGAAGTGGTGGCGAGGCAATGGCTGCCCAGAGGAGGAGAGCAAAGAGGCCAGTGCTCTAGGGGT GCAGAATATTGGTGGTATCTTCATTGTCCTGGCAGCCGGCTTGGTGCTCTCAGTTTTTGTGGCAGTGGGA GAGTTTTTATACAAATCCAAAAAAAACGCTCAATTGGAAAAGAGGTCCTTCTGTAGCGCCATGGTGGAAG AACTGAGAATGTCTCTGAAGTGCCAGCGTCGGCTCAAACATAAGCCACAGGCCCCAGTTATTGTGAAAAC AGAAGAAGTTATCAACATGCACACATTTAACGACAGAAGGTTGCCAGGTAAAGAAACCATGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219234 representing NM_001111268
Red=Cloning site Green=Tags(s) MKIISPVLSNLVFSRSIKVLLCLLWIGYSQGTTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTL LPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSD NKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKD AKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFR ILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRH KPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPSSGLNMTESQKGK PANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGA QDDVNGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLS PDIWMYVLLACLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMRQGSELMPKA LSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKIS TYDKMWAFMSSRRQSVLVKSNEEGIQRVLTSDYAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPM GSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEESKEASALGVQNIGGIFIVLAAGLVLSVFVAVG EFLYKSKKNAQLEKRSFCSAMVEELRMSLKCQRRLKHKPQAPVIVKTEEVINMHTFNDRRLPGKETMA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001111268 |
| ORF Size | 2724 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111268.2 |
| RefSeq Size | 4785 bp |
| RefSeq ORF | 2727 bp |
| Locus ID | 14806 |
| MW | 102.9 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222511 | Grik2 (untagged) - Mouse glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), transcript variant 1, (10ug) |
CNY 11,690.00 |
|
| MG219234 | Grik2 (tGFP-tagged) - Mouse glutamate receptor ionotropic kainate 2 (beta 2) (Grik2) transcript variant 1, (10ug) |
CNY 8,650.00 |
|
| MR219234L3 | Lenti ORF clone of Grik2 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), transcript variant 1 |
CNY 9,790.00 |
|
| MR219234L4 | Lenti ORF clone of Grik2 (mGFP-tagged) - Mouse glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), transcript variant 1 |
CNY 12,376.00 |
