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Akirin2 (NM_001007589) Mouse Tagged ORF Clone
CAT＃: MR219891
- TrueORF®
Akirin2 (Myc-DDK-tagged) - Mouse akirin 2 (Akirin2)
ORF Plasmid: tGFP
"NM_001007589" in other vectors (4)
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CNY 1,416.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2700059D21Rik; AA114675; AA522011; Akirin-2; AU019887 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219891 representing NM_001007589
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGCGGAGCCACTCTGAAAAGGACTCTGGATTTCGACCCGCTGCTGAGCCCCGCATCTCCGAAGC GGAGGCGGTGCGCGCCTTTGTCGGCGCCGGCTTCGGCTGCCGCCTCCCCTGCCGCCGCCACCGCCGCCGC CGCCGCCTCGGCCGCGGCCGCCTCGCCGCAGAAGTATCTCCGGATGGAGCCTTCCCCCTTCGGCGACGTC TCTTCCCGGCTCACCACAGAACAAATTCTGTACAACATAAAACAGGAATATAAACGCATGCAGAAGAGAA GACATTTAGAAGCTAGTTTTCAGCAGGCAGATCCAGGTTGTACTTCCGATTCACAGCCACATGCATTTCT CATCAGTGGACCAGCATCACCAGGGACTTCATCTGCAACATCCTCACCATTAAAAAAAGAACAGCCCTTA TTTACTCTAAGGCAGGTTGGAATGATCTGTGAACGTTTGTTGAAAGAACGGGAAGAGAAAGTTCGAGAAG AATATGAAGAAATACTGAATACAAAACTTGCAGAACAATATGATGCTTTTGTGAAGTTTACGCATGATCA GATAATGCGGCGATATGGAGAACAGCCTGCTAGTTATGTTTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219891 representing NM_001007589
Red=Cloning site Green=Tags(s) MACGATLKRTLDFDPLLSPASPKRRRCAPLSAPASAAASPAAATAAAAASAAAASPQKYLRMEPSPFGDV SSRLTTEQILYNIKQEYKRMQKRRHLEASFQQADPGCTSDSQPHAFLISGPASPGTSSATSSPLKKEQPL FTLRQVGMICERLLKEREEKVREEYEEILNTKLAEQYDAFVKFTHDQIMRRYGEQPASYVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007589 |
| ORF Size | 603 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007589.3, NP_001007590.2 |
| RefSeq Size | 1729 bp |
| RefSeq ORF | 606 bp |
| Locus ID | 433693 |
| UniProt ID | B1AXD8 |
| MW | 22.6 kDa |
| Gene Summary | Forms a complex with YWHAB that acts to repress transcription of DUSP1 (By similarity). Required for embryonic development and the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A critical role for the nuclear protein Akirin2 in the formation of mammalian muscle in vivo
,Bosch, PJ;Fuller, LC;Weiner, JA;,
Genesis
,PubMed ID 30801883
[AKIRIN2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214975 | Akirin2 (untagged) - Mouse akirin 2 (Akirin2), (10ug) |
CNY 3,990.00 |
|
| MG219891 | Akirin2 (tGFP-tagged) - Mouse akirin 2 (Akirin2), (10ug) |
CNY 2,850.00 |
|
| MR219891L3 | Lenti ORF clone of Akirin2 (Myc-DDK-tagged) - Mouse akirin 2 (Akirin2) |
CNY 4,750.00 |
|
| MR219891L4 | Lenti ORF clone of Akirin2 (mGFP-tagged) - Mouse akirin 2 (Akirin2) |
CNY 4,750.00 |
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