Tmed1 (NM_010744) Mouse Tagged ORF Clone

CAT#: MR220012

  • TrueORF®

Tmed1 (Myc-DDK-tagged) - Mouse transmembrane emp24 domain containing 1 (Tmed1)

ORF Plasmid: DDK tGFP



  "NM_010744" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 1,416.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


TMED1 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Il1rl1l; Ly84l; St2l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR220012 representing NM_010744
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCGGCCGGCGCGGCCGTAGCACTGGCCCTGTGGCTACTCCTGCCAGCAGTAGGAGTGGGAGAGG
CAGGGCCGCCGCCTATCCAGGACGGCGAGTTCACATTTCTGCTTCCCGCCGGGAGAAAGCAGTGTTTCTA
TCAGTCCGCACCGGCCAATGCTAGTCTTGAGACCGAGTACCAGGTGATCGGAGGTGCTGGGCTGGACGTG
GACTTCACCTTGGAGAGCCCTCAGGGTGTGCTGTTGGTCAGTGAGTCTCGAAAGGCTGATGGGGTACACA
CGGTGGAGCCTACTGAGGCCGGAGACTACAGGCTGTGCTTTGACAACTCCTTCAGCACCATCTCAGAAAA
GCTTGTGTTCTTTGAGCTCATCTTTGACAGCTTCCAAGATGAGGAGGAGGTAGAAGGTTGGGCGGAGGCT
GTGGAGCCAGAAGAGATGCTTGATGTCAAAATGGAAGACATCAAGGAATCCATAGAGACCATGAGGACCC
GGCTGGAACGGAGCATCCAGATGCTCACTCTGCTCCGAGCCTTTGAGGCTCGTGATCGAAATCTTCAAGA
AGACAACCTGGAGCGGGTCAACTTCTGGTCAGCTGCCAATGTGGCTGTGTTGCTGCTGGTGGCTGTCCTG
CAAGTCTGCACACTCAAGCGCTTCTTCCATGACAAGCGCCCTGTACCCACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR220012 representing NM_010744
Red=Cloning site Green=Tags(s)

MMAAGAAVALALWLLLPAVGVGEAGPPPIQDGEFTFLLPAGRKQCFYQSAPANASLETEYQVIGGAGLDV
DFTLESPQGVLLVSESRKADGVHTVEPTEAGDYRLCFDNSFSTISEKLVFFELIFDSFQDEEEVEGWAEA
VEPEEMLDVKMEDIKESIETMRTRLERSIQMLTLLRAFEARDRNLQEDNLERVNFWSAANVAVLLLVAVL
QVCTLKRFFHDKRPVPT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010744
ORF Size 681 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010744.4, NP_034874.2
RefSeq Size 1487 bp
RefSeq ORF 684 bp
Locus ID 17083
UniProt ID Q3V009
MW 25.7 kDa
Gene Summary Potential role in vesicular protein trafficking, mainly in the early secretory pathway. May act as a cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and may be involved in vesicle coat formation at the cytoplasmic side.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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