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Grin2d (NM_008172) Mouse Tagged ORF Clone
CAT＃: MR220972
- TrueORF®
Grin2d (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2D (epsilon 4) (Grin2d)
ORF Plasmid: tGFP
"NM_008172" in other vectors (4)
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CNY 9,216.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GluN2D; NMDAR2D; NR2D |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220972 representing NM_008172
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGGCGCCGGTGGCCCCCGCGGCCCTCGGGGCCCCGCTAAGATGCTGTTGCTGCTGGCGCTGGCGT GCGCCAGCCCGTTCCCGGAGGAGGTGCCGGGGCCGGGCGCGGCCGGCGGGGGCACGGGCGGGGCGCGGCC GCTCAACGTGGCGCTGGTCTTCTCTGGCCCGGCGTACGCGGCCGAGGCGGCGCGCTTGGGCCCGGCCGTG GCGGCGGCAGTGCGCAGCCCGGGCCTGGACGTACGGCCCGTGGCGCTGGTGCTCAACGGCTCCGACCCTC GCAGCCTTGTGCTGCAGCTCTGCGACCTGCTGTCGGGGCTGCGCGTGCACGGCGTGGTGTTCGAGGACGA CTCGCGCGCGCCCGCCGTCGCGCCCATTCTCGACTTCCTGTCGGCGCAGACCTCGCTGCCCATCGTGGCC GTGCACGGCGGCGCCGCGCTCGTACTCACACCCAAGGAGAAGGGCTCCACCTTCCTGCAGCTTGGCTCCT CCACAGAGCAACAGCTGCAGGTCATTTTTGAGGTGCTGGAGGAGTACGACTGGACATCCTTTGTGGCAGT GACTACGCGTGCCCCAGGCCATCGAGCCTTCTTGTCATACATCGAGGTGCTGACTGATGGCAGCCTGGTG GGCTGGGAGCATCGAGGAGCGCTGACACTGGACCCCGGAGCGGGTGAGGCCGTCCTGGGCGCACAGCTCC GTAGTGTCAGTGCGCAGATCCGCCTGCTCTTCTGCGCCCGCGAGGAGGCAGAGCCTGTTTTCCGGGCGGC AGAAGAGGCTGGTCTCACTGGGCCTGGCTACGTCTGGTTCATGGTGGGACCTCAGCTGGCCGGAGGTGGG GGCTCCGGGGTCCCTGGGGAACCACTTCTTCTGCCAGGAGGTGCCCCACTGCCTGCTGGGCTGTTTGCAG TGCGCTCTGCTGGCTGGCGTGACGACTTGGCACGTCGAGTGGCTGCTGGTGTGGCGGTGGTGGCCAGAGG TGCCCAGGCCCTGCTGCGAGACTATGGCTTCCTGCCTGAGCTGGGCCATGACTGTCGCGCCCAGAATCGC ACCCACCGCGGGGAGAGTCTGCACAGGTATTTCATGAACATCACCTGGGATAACCGAGACTACTCCTTCA ATGAGGATGGCTTTCTGGTAAACCCGTCACTGGTAGTCATCTCCCTCACCAGAGACAGGACGTGGGAAGT GGTGGGCAGCTGGGAACAGCAGACCCTCCGCCTCAAGTACCCTCTATGGTCCCGCTATGGCCGCTTCCTG CAGCCGGTGGATGACACGCAGCACCTCACTGTGGCCACGCTGGAGGAGAGACCTTTTGTCATTGTAGAGC CTGCAGACCCCATCAGCGGCACTTGCATCAGAGACTCGGTTCCCTGCCGGAGCCAGCTCAACCGTACCCA CAGCCCTCCGCCTGACGCTCCCCGCCCGGAGAAGAGATGCTGCAAGGGTTTCTGCATTGACATTTTGAAG AGGCTGGCGCACACCATCGGTTTCAGCTATGACCTCTACCTGGTTACCAACGGCAAGCATGGCAAGAAGA TCGATGGCGTCTGGAATGGCATGATTGGTGAGGTGTTCTATCAGCGTGCGGACATGGCCATCGGCTCCCT CACCATCAATGAGGAGCGGTCAGAGATCGTGGACTTCTCCGTCCCTTTTGTAGAGACAGGCATCAGCGTC ATGGTGGCACGCAGCAATGGCACTGTGTCCCCCTCTGCCTTCCTCGAGCCCTACAGCCCCGCTGTGTGGG TGATGATGTTCGTCATGTGCCTCACCGTGGTCGCCGTCACAGTTTTCATCTTTGAGTACCTCAGTCCTGT GGGCTATAACCGAAGCCTGGCCACGGGCAAACGCCCCGGAGGCTCTACCTTCACCATTGGGAAATCCATC TGGCTGCTGTGGGCCCTGGTGTTCAACAACTCCGTGCCAGTGGAGAATCCTCGGGGCACCACCAGCAAGA TCATGGTGCTGGTGTGGGCCTTCTTTGCCGTCATCTTTCTTGCCAGCTATACAGCCAATCTGGCTGCCTT CATGATCCAGGAGGAGTACGTGGACACCGTGTCTGGGCTCAGCGACCGGAAGTTCCAGCGGCCCCAGGAG CAATACCCACCCCTGAAGTTTGGAACGGTGCCCAATGGGTCCACGGAGAAGAATATCCGAAGCAACTACC CTGATATGCACAGCTACATGGTGCGATACAACCAGCCAAGAGTGGAGGAGGCGCTCACTCAGCTCAAGGC AGGGAAACTGGACGCCTTCATCTATGACGCAGCAGTGCTCAACTACATGGCCCGAAAGGATGAGGGCTGC AAGCTGGTCACCATCGGCTCAGGCAAGGTCTTCGCCACCACTGGTTATGGCATCGCCCTACACAAGGGCT CCCGCTGGAAGAGGCCCATCGACCTGGCGCTGCTGCAGTTCCTGGGGGACGATGAGATTGAGATGCTGGA GCGGCTGTGGCTTTCAGGGATCTGCCACAACGACAAAATCGAGGTGATGAGCAGCAAGCTGGATATCGAC AACATGGCAGGTGTCTTCTACATGCTCCTCGTGGCCATGGGCCTCTCCTTGCTGGTCTTCGCCTGGGAAC ACCTTGTGTACTGGCGACTGCGGCACTGTCTGGGGCCCACCCACCGCATGGATTTCCTACTGGCCTTCTC CAGGGGTATGTACAGCTGCTGCAGCGCTGAGGCTGCTCCGCCGCCGGCCAAACCCCCGCCACCGCCGCAG CCGCTGCCCAGTCCGGCGTATCCCGCCGCTCGCCCACCCCCTGGCCCCGCACCCTTCGTGCCCCGAGAGC GCGCAGCCGCCGACCGCTGGCGCCGGGCCAAGGGCACAGGGCCCCCGGGGGGCGCAGCGCTAGCCGACGG CTTCCACCGATACTACGGCCCCATCGAGCCGCAGGGGCTGGGCCTCGGCGAGGCGCGCGCGGCACCGAGA GGCGCAGCCGGACGCCCACTGTCCCCACCCACCACACAGCCCCCACAGAAGCCACCACCTTCCTACTTCG CCATCGTGCGCGAGCAAGAGCCGGCCGAGCCCCCCGCCGGCGCCTTCCCGGGCTTCCCATCTCCGCCCGC TCCGCCTGCCGCCGCAGCCGCCGCCGTCGGGCCGCCACTGTGCCGCCTGGCTTTCGAGGACGAGAGCCCG CCCGCGCCCTCGCGCTGGCCGCGTTCTGACCCCGAGAGCCAGCCGCTGTTGGGTGGGGGCGCGGGCGGCC CGAGCGCTGGGGCCCCGACCGCACCACCGCCGCGCCGCGCCGCGCCACCACCGTGCGCCTACCTGGACCT CGAGCCTTCGCCTTCGGACTCCGAGGATTCGGAGAGCCTGGGCGGAGCGTCGCTCGGTGGCCTGGAGCCC TGGTGGTTCGCCGACTTCCCCTACCCGTATGCGGAGCGCCTCGGGCCGCCGCCCGGCCGCTACTGGTCGG TTGACAAGCTCGGGGGCTGGCGCGCTGGTAGCTGGGACTACCTGCCCCCGCGCGGCGGCCCCGCATGGCA CTGCCGCCACTGCGCCAGCCTGGAGCTGCTACCGCCACCACGCCATCTCAGCTGCTCGCACGACGGCCTA GACGGTGGCTGGTGGGCGCCTCCGCCTCCACCCTGGGCTGCGGGGCCACCGCCCCGGCGCCGGGCGCGCT GTGGTTGTCCGCGACCGCACCCGCACCGCCCCCGGGCTTCGCACCGTGCGCCCGCCGCCGCACCGCACCA CCACCGACACAGGCGCGCGGCGGGTGGCTGGGACCTCCCGCCGCCCGCGCCCACCTCGCGTTCGCTGGAG GACCTGAGCTCCTGCCCACGGGCGGCCCCCACGCGCAGGCTCACCGGGCCCTCGCGCCACGCGCGCCGCT GTCCGCACGCTGCGCATTGGGGGCCGCCCCTGCCCACCGCATCTCACCGGAGACACCGGGGCGGGGACCT GGGCACACGCAGGGGCTCTGCGCACTTCTCCAGCCTGGAGTCCGAGGTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR220972 representing NM_008172
Red=Cloning site Green=Tags(s) MRGAGGPRGPRGPAKMLLLLALACASPFPEEVPGPGAAGGGTGGARPLNVALVFSGPAYAAEAARLGPAV AAAVRSPGLDVRPVALVLNGSDPRSLVLQLCDLLSGLRVHGVVFEDDSRAPAVAPILDFLSAQTSLPIVA VHGGAALVLTPKEKGSTFLQLGSSTEQQLQVIFEVLEEYDWTSFVAVTTRAPGHRAFLSYIEVLTDGSLV GWEHRGALTLDPGAGEAVLGAQLRSVSAQIRLLFCAREEAEPVFRAAEEAGLTGPGYVWFMVGPQLAGGG GSGVPGEPLLLPGGAPLPAGLFAVRSAGWRDDLARRVAAGVAVVARGAQALLRDYGFLPELGHDCRAQNR THRGESLHRYFMNITWDNRDYSFNEDGFLVNPSLVVISLTRDRTWEVVGSWEQQTLRLKYPLWSRYGRFL QPVDDTQHLTVATLEERPFVIVEPADPISGTCIRDSVPCRSQLNRTHSPPPDAPRPEKRCCKGFCIDILK RLAHTIGFSYDLYLVTNGKHGKKIDGVWNGMIGEVFYQRADMAIGSLTINEERSEIVDFSVPFVETGISV MVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVTVFIFEYLSPVGYNRSLATGKRPGGSTFTIGKSI WLLWALVFNNSVPVENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEEYVDTVSGLSDRKFQRPQE QYPPLKFGTVPNGSTEKNIRSNYPDMHSYMVRYNQPRVEEALTQLKAGKLDAFIYDAAVLNYMARKDEGC KLVTIGSGKVFATTGYGIALHKGSRWKRPIDLALLQFLGDDEIEMLERLWLSGICHNDKIEVMSSKLDID NMAGVFYMLLVAMGLSLLVFAWEHLVYWRLRHCLGPTHRMDFLLAFSRGMYSCCSAEAAPPPAKPPPPPQ PLPSPAYPAARPPPGPAPFVPRERAAADRWRRAKGTGPPGGAALADGFHRYYGPIEPQGLGLGEARAAPR GAAGRPLSPPTTQPPQKPPPSYFAIVREQEPAEPPAGAFPGFPSPPAPPAAAAAAVGPPLCRLAFEDESP PAPSRWPRSDPESQPLLGGGAGGPSAGAPTAPPPRRAAPPPCAYLDLEPSPSDSEDSESLGGASLGGLEP WWFADFPYPYAERLGPPPGRYWSVDKLGGWRAGSWDYLPPRGGPAWHCRHCASLELLPPPRHLSCSHDGL DGGWWAPPPPPWAAGPPPRRRARCGCPRPHPHRPRASHRAPAAAPHHHRHRRAAGGWDLPPPAPTSRSLE DLSSCPRAAPTRRLTGPSRHARRCPHAAHWGPPLPTASHRRHRGGDLGTRRGSAHFSSLESEV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008172 |
| ORF Size | 3969 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008172.2, NP_032198.2 |
| RefSeq Size | 4383 bp |
| RefSeq ORF | 3972 bp |
| Locus ID | 14814 |
| UniProt ID | Q03391 |
| MW | 143.4 kDa |
| Gene Summary | Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1385220). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Subunit-specific effects of poricoic acid A on NMDA receptors
,Lee, J;Kim, C;Yeom, HD;Nguyen, KVA;Eom, S;Lee, S;Jung, JH;Lee, JH;Kim, SH;Kim, IK;Lee, JH;,
Pharmacol Rep
,PubMed ID 32048268
[GRIN2D]
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Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224232 | Grin2d (untagged) - Mouse glutamate receptor, ionotropic, NMDA2D (epsilon 4) (Grin2d), (10ug) |
CNY 17,010.00 |
|
| MG220972 | Grin2d (tGFP-tagged) - Mouse glutamate receptor ionotropic NMDA2D (epsilon 4) (Grin2d), (10ug) |
CNY 10,550.00 |
|
| MR220972L3 | Lenti ORF clone of Grin2d (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, NMDA2D (epsilon 4) (Grin2d) |
CNY 11,500.00 |
|
| MR220972L4 | Lenti ORF clone of Grin2d (mGFP-tagged) - Mouse glutamate receptor, ionotropic, NMDA2D (epsilon 4) (Grin2d) |
CNY 11,500.00 |
