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Polr3a (NM_001081247) Mouse Tagged ORF Clone
CAT＃: MR221294
- TrueORF®
Polr3a (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide A (Polr3a)
ORF Plasmid: tGFP
"NM_001081247" in other vectors (4)
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CNY 9,512.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9330175N20Rik; BC053071; RPC1; RPC155 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221294 representing NM_001081247
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAGGAGCAGTTCCGCGAGACGGATGTGGCTAAGAAAATAAGCCATATCTGTTTTGGAATGAAGT CCCCTGAGGAGATGCGCCAGCAGGCACATATCCAGGTTGTCAGTAAGAACCTGTACAGCCAAGACAACAA CCACGCCCCCTTGTTGTATGGAGTGCTTGACCATAGAATGGGTACGAGCGAAAAAGATCGTCCTTGTGAG ACCTGTGGGAAGAACTTGGCGGACTGTCTCGGCCACTATGGCTACATTGATCTCGAGCTACCGTGCTTTC ATGTCGGGTACTTCAGGGCTGTCATTGGTATCTTACAGATGATCTGCAAAACCTGCTGCCACATTATGCT GTCCCAGGAAGAGAAGCAGCAGTTTCTAGATTTTCTGAAGAGGCCTGGCCTAACCTACCTGCAGAAGCGA GGCCTGAAGAAGAAGATCTCAGATAAATGCCGGAAGAAAAGCACTTGTCACTACTGTGGTGCCTTTAATG GTACCGTAAAGAAATGTGGTTTACTGAAGATAATTCATGAAAAATACAAGACAAACAAAAAAGTCGTGGA TCCCATTGTATCAAACTTCCTGCAGTCCTTTGAGACAGCCATTGAGCATAACAAAGAGGTTGAACCCCTG CTGGGAAGGGCCCAGGAGAACTTGAATCCTTTAGTAGTTCTGAACTTGTTTAAGCGCATCCCAGCTGAAG ATGTCCCGTTACTCCTGATGAACCCAGAATCTGGAAAGCCCTCTGATTTGATTCTCACCCGACTTTTGGT GCCTCCCCTGTGTATCAGACCGTCCGTCGTGAGTGATCTGAAGTCTGGAACCAATGAAGATGACCTGACA ATGAAACTGACAGAGATCATTTTCCTAAATGATGTTATTAAAAAGCACCGGATCTCCGGAGCCAAGACGC AGATGATCATGGAGGACTGGGATTTCCTGCAGCTGCAGTGTGCCCTCTACATCAACAGTGAGCTCTCTGG CATCCCTCTCAACATGGCACCTAAGAAATGGACGCGAGGCTTTGTGCAGCGCCTGAAGGGGAAACAGGGT CGATTTAGAGGTAATCTGTCAGGGAAGAGGGTGGATTTCTCCGGCCGAACAGTCATCTCACCTGACCCCA ACCTCCGGATTGATGAAGTAGCCGTGCCAGTTCATGTGGCCAAAATTCTAACTTTTCCTGAGAAGGTGAA CAAAGCAAACATCAACTTTTTGAGGAAACTGGTTCGAAATGGCCCAGATGTTCACCCAGGAGCCAATTTT ATCCAACAAAGACACATGCAGATGAAGAGGTTCCTGAAGTATGGAAATCGGGAAAAGATGGCTCAGGAGC TCAAGTTCGGCGACATTGTAGAGAGACACCTGATAGATGGAGATGTGGTCTTGTTCAACCGGCAGCCCTC CCTGCACAAACTGAGCATCATGGCCCACCTGGCCAGGGTTAAGCCCCACCGGACCTTCAGATTCAATGAA TGTGTCTGCACCCCCTACAATGCAGACTTTGATGGGGATGAAATGAACCTTCATCTTCCCCAGACAGAGG AAGCCAAAGCAGAGGCCCTTGTTCTCATGGGGACCAAAGCGAACCTTGTAACCCCACGAAATGGGGAGCC ACTCATTGCTGCCATTCAGGACTTCTTAACAGGTGCTTACCTCCTCACCCTCAAGGACACCTTCTTTGAC CGAGCCAAGGCTTGCCAGATCATCGCTTCAATACTGGTTGGCAAAGATGAAAAAATTAAAGTGCGCCTCC CACCCCCTACAATACTAAAGCCTGTCACCCTGTGGACCGGAAAGCAGATCTTCAGTGTCATCCTCAGACC TAGTGATGACAATCCAGTGCGGGCCAACCTCCGAACCAAGGGCAAGCAGTACTGTGGCAAGGGGGAAGAC CTCTGCGTCAACGACTCCTATGTCACCATCCAGAACAGTGAGTTGATGTGCGGCAGCATGGACAAAGGGA CCCTGGGGTCGGGGTCCAAGAACAACATCTTCTACATCTTGCTACGAGACTGGGGGCAGAATGAGGCTGC CGATGCCATGTCTCGGCTTGCCAGGCTGGCTCCTGTCTACCTGTCTAACCGTGGGTTCTCCATCGGGATT GGTGATGTCACGCCTGGCCAGGGACTGCTGAAAGCCAAGTATGAGCTACTAAATGCTGGCTACAAAAAAT GTGACGAGTACATTGAAGCCCTGAACACAGGCAAGCTGCAGCAGCAGCCAGGCTGCACGGCCGAGGAGAC GCTTGAGGCTCTGATCCTGAAGGAGCTGTCTGTGATCCGGGACCATGCTGGCAGTGCCTGCCTCCGCGAG CTGGACAAGAGCAACAGCCCCCTCACCATGGCCCTGTGTGGCTCCAAAGGTTCCTTCATAAACATATCAC AGATGATTGCCTGTGTGGGACAGCAAGCCATCAGTGGCTCCCGGGTTCCAGATGGCTTTGAGAACAGATC TTTGCCTCACTTTGAGAAGCATTCAAAGCTCCCTGCTGCCAAAGGCTTTGTGGCCAATAGCTTTTACTCT GGCTTAACGCCTACTGAGTTTTTCTTCCACACAATGGCCGGTCGGGAGGGTCTGGTGGACACAGCTGTAA AGACTGCTGAAACAGGATACATGCAGAGAAGACTGGTCAAATCCCTGGAAGACCTCTGCTCGCAGTACGA TCTGACAGTGCGGAGCTCCACCGGTGACATCATCCAGTTCATCTATGGAGGAGATGGCTTAGATCCTGCT GCCATGGAGGGCAAAGACGAACCGCTGGAGTTTAAGCGGGTTCTGGACAACATCAAGGCAGTGTATCCAT GTCAGACTGAACCTGCTCTCAGTAAGAACGACCTCACCTTGACCGCTGAGGCCATCATGAAGAAGAATGA GTTCCTATGCTGCCAAGACAGCTTCCTGCAGGAAATAAAAACATTCATTAAAGGGGTGTCCGAGAAGATT AAGAAAACCAGAGATAAATATGGGATCAATGATAACGGCACAACAGAGCCTCGTGTGCTGTACCAGTTGG ACCGTATTACCCCTACCCAAATAGAGAAGTTTCTGGAGACCTGTAGGGATAAGTATATGAGAGCACAGAT GGAGCCAGGCTCTGCGGTAGGTGCACTCTGTGCACAGAGCATTGGTGAGCCCGGCACCCAGATGACGCTG AAGACATTCCACTTTGCAGGAGTAGCCTCCATGAACATCACCCTGGGTGTGCCTCGGATCAAAGAGATCA TCAACGCTTCCAAGGCCATCAGCACTCCAATTATCACAGCACAGCTAGACAAGGATGACGACGCGGATTA TGCACGCCTGGTGAAGGGCAGGATTGAGAAAACCCTCCTGGGGGAGATCTCAGAGTATATTGAAGAAGTG TTTCTTCCTGATGACTGCTTTATTCTTGTCAAGCTCTCCCTGGAACGGATCCGACTTCTGAGACTGGAGG TCAATGCTGAGACAGTGCGGTACTCCATCTGCACATCCAAGCTCCGAGTGAAGCCTGGTGATGTGGCCGT TCATGGGGAGGCTGTGGTGTGTGTCACCCCCAGGGAAAACAGCAAGAGCTCCATGTATTATGTGCTGCAG TTTCTCAAAGAGGATCTGCCCAAGGTGGTTGTACAAGGCATCCCAGAGGTGTCGCGAGCTGTCATCCATA TTGACGAGCAGAGTGGGAAGGAGAAGTTCAAGCTTCTGGTGGAAGGTGACAACCTGCGGGCAGTCATGGC CACCCACGGTGTGAAAGGCACCCGGACCACGTCTAATAACACCTATGAGGTGGAAAAAACCCTGGGCATT GAGGCTGCCCGGACAACTATCATCAACGAGATCCAGTACACGATGGTCAACCACGGCATGAGCATCGACA GGAGGCACGTGATGCTGCTCTCTGACCTGATGACCTACAAGGGTGAAGTCCTGGGTATAACCAGGTTTGG CCTGGCCAAGATGAAGGAGAGTGTGTTGATGCTGGCCTCCTTTGAAAAGACAGCTGACCATCTCTTTGAT GCTGCCTACTTTGGGCAGAAAGACTCTGTATGCGGAGTGTCTGAGTGCATCATCATGGGAATCCCGATGA ACATCGGAACTGGGCTCTTCAAGCTGTTACACAAAGCCAACAGGGACCCCAAACCACCCAGGAGACCCCT GATATTTGACACACATGAATTCCACATCCCTCTTGTTACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221294 representing NM_001081247
Red=Cloning site Green=Tags(s) MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNNHAPLLYGVLDHRMGTSEKDRPCE TCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKQQFLDFLKRPGLTYLQKR GLKKKISDKCRKKSTCHYCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIEHNKEVEPL LGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPESGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLT MKLTEIIFLNDVIKKHRISGAKTQMIMEDWDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQG RFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVRNGPDVHPGANF IQQRHMQMKRFLKYGNREKMAQELKFGDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNE CVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFD RAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGED LCVNDSYVTIQNSELMCGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGI GDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHAGSACLRE LDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYS GLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPA AMEGKDEPLEFKRVLDNIKAVYPCQTEPALSKNDLTLTAEAIMKKNEFLCCQDSFLQEIKTFIKGVSEKI KKTRDKYGINDNGTTEPRVLYQLDRITPTQIEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTL KTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEV FLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQ FLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKFKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGI EAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFD AAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKANRDPKPPRRPLIFDTHEFHIPLVT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081247 |
| ORF Size | 4170 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081247.2 |
| RefSeq Size | 4683 bp |
| RefSeq ORF | 4173 bp |
| Locus ID | 218832 |
| MW | 155.7 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224387 | Polr3a (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide A (Polr3a), (10ug) |
CNY 17,860.00 |
|
| MG221294 | Polr3a (tGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide A (Polr3a), (10ug) |
CNY 13,490.00 |
|
| MR221294L3 | Lenti ORF clone of Polr3a (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide A (Polr3a) |
CNY 14,160.00 |
|
| MR221294L4 | Lenti ORF clone of Polr3a (mGFP-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide A (Polr3a) |
CNY 14,160.00 |
