Ltbp4 (NM_175641) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310046A13Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222718 representing NM_175641 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGGCCTGGCCTCGGCGGCCCCTGCCCCCTCTTGCTGCTGCTGCTGCTCCCAGCCGCCACATCTG CTAGCGGCTCCAGTCCCAGCCCCAGCCCCAGCCCCATCGAGAAAGCGGTGGTCCCGAGCCACCAGGCCGG TGTTGCTGCCTGTCACTGCTGCCTAGACCAGACACCTAAGAGTAGCCGCTGCACCCGAGCTTCCTGCAGG GTCCGGAACTGCCCACCTGCCAAGTGCACAGGTCTAGAGGGGTGCCTGACCCCAACACCTTCGGTGCCCA GCCCCAGCCGCAGTCCCGTGGAGAAGAGTCAGGTATCCCTCAACTGGCAGCCACTGACTTTGCAGGAGGC CCGAGCTCTGCTGAGGCAAAGGCGGCCCCGAGGGCCGTGGGCCCGGGCATTGCTGAAAAGAAGGCCCCCA CATCGCGCCCCTGCCGGCCAGGCCCGGGTCCTATGTCCCTTGATCTGTCACAACGGTGGTGTGTGTGTGA AGCCTGATCGCTGCCTTTGCCCCCCGGACTTCGCTGGCAAATTCTGCCAATTGCATTCTTCGGGGGCCCG GCCCCCGGCCCCAGCCATGCCGGGCCTTACCCGCTCCGTTTATACAATGCCACTAGCCAACCACCGCGAT GATGAACACGGAGTAGCATCGATGGTGAGCGTGCACGTGGAGCACCCTCAGGAGGCATCAGTGGTGGTGC ACCAAGTGGAACGTGTGTCGGGCCCCTGGGAGGAGGCGAACCCTGAAGCCCTAGCCAGGGCTGAAGCGGC GGCACGGGCCGAGGCGGCGGCGCCCTACACAGTGCTGGCACAGAGCGCGCCACGCGAGGACGGCTACTCA GACGCCTCCGGCTTCGGTTACTGTTTTAGAGAACTGCGCGGAAGCGAATGTGCATCGCCGCTGCCAGGGC TCCGGACGCAGGAAGTCTGCTGCCGAGGAGAGGGCTTGGCCTGGGGTGTTCATGATTGTCACCCGTGCGC GGAACACCTGAGGAACTCCAACCAAGTGAGTGGCCCAAATGGACCTTGTCCACCCGGCTTCGAGAGAGTT AATGGGTCCTGTGTAGATGTGGATGAGTGCGCCACCGGTGGGCGTTGCCAACACGGGGAGTGTGCCAACA CTCGTGGCGGCTACACGTGTGTATGTCCGGACGGTTTCCTCCTGGACTCTTCCCGCAGCAGCTGCATCTC CCAACACGTGATCTCCGAGGCAAAGGGGCCCTGCTACCGAGTGCTGCATGATGGCGGATGCTCGCTGCCC ATTCTTCGAAATATCACCAAACAGATCTGCTGCTGTAGCCGTGTGGGCAAGGCGTGGGGCCGAGGCTGCC AGCTCTGTCCACCTTATGGTTCAGAGGGTTTTCGGGAGATCTGTCCAGCTGGCCCTGGCTACCACTATTC AGCTTCTGACCTCCGATACAACACCAGACCCCTAAACCAGGATCCACCTCGAGTGACCTTCAATCAGCCA CGTGTTCCACCAGCCACCCCTCGGCCACCTACAGGCTTTCTGCCTACTCGTCGACCTGAGCCCCGCCCTG ACCCTGGGCCACAGCCTGAACCCCGGCCTCGGCCTGAACCCCGGCCTCGGCCTGAATCCCGACCTCGGCC TGAACCCCGGCCTCGGCCTGAACCCCGGCCTCAGCCTGAATCCCAGCCTAGGCCTGAATCCCGGCCTCGG CCTGAATCCCAGCCTTGGCCTGAATTTCCCCTGCCCAGCATCCCTGCTTGGACCGGTCCAGAGATTCCTG AATCAGGTCCCTCTTCCAGCATGTGTCAGCGAAATCCCCAGGTTTGTGGTCCTGGACGCTGCGTTCCTAG GCCAAGCGGCTACACTTGTGCGTGCGACCCAGGTTTCCGGCTCGGCCCCCAGGGCACTCGCTGCATTGAC ATAGATGAATGTCGCCGCGTCCCTACACCCTGTGCTCCTGGGCGTTGCGAGAATACACCAGGCAGCTTTC GCTGCGTGTGCGGCACGGGCTTCCAAGCAGGCCCGCGGGCTACAGAGTGCCTGGATGTGGACGAGTGCCG CCGCGTGCCGCCGCCGTGTGACCGCGGGCGCTGCGAGAACACGCCAGGCAGTTTCCTATGTGTGTGTCCC GCCGGGTACCAGGCAGCACCGCACGGAGCCAGCTGCCAGGATGTGGATGAATGCACCCAGAGCCCAGGCC TCTGTGGTCGCGGGGTCTGCGAAAACCTGCCCGGCTCTTTCCGCTGTGTTTGCCCGGCTGGCTTCCGGGG CTCGGCGTGTGAAGAAGATGTGGATGAATGTGCCCAGCAGCCTCCGCCTTGCGGGCCAGGCCGCTGCGAC AACACCGCGGGTTCGTTCCACTGTGCCTGCCCAGCTGGCTTCCGATCCCGAGGACCAGGAGCCCCCTGCC AAGATGTGGATGAATGTTCCCGGAGCCCCTCGCCTTGTGCCTATGGCCGCTGTGAGAACACGGAAGGCAG CTTCAAGTGTGTCTGCCCAACAGGCTTCCAACCCAACGCTGCGGGCTCCGAGTGCGAGGATGTGGATGAG TGTGAGAACCGCTTGGCGTGTCCAGGGCAGGAGTGTGTGAACTCACCGGGCTCCTTCCAGTGCCGGGCCT GCCCTGTTGGGCACCACCTGCACCGTGGCAGATGTACTGATGTGGATGAATGCAGTTCGGGCACCCCTTG TGGTCTCCATGGCCAGTGCACAAATACTAAAGGCTCCTTCCACTGCAGCTGCTCGACAGGTTACCGGGCG CCATCTGGTCAACCTGGGCCTTGTGCAGACATAAATGAGTGTCTGGAAGGCGACTTCTGCTTCCCCCACG GAGAGTGCCTCAACACTGATGGCTCCTTTACCTGTACCTGTGCCCCTGGCTACCGGCCTGGACCTCGTGG AGCATCTTGTCTGGACGTGGACGAGTGCAGCGAAGAGGATCTTTGCCAGAGCGGCATCTGTACCAACACT GACGGCTCCTTCGAGTGCATCTGTCCTCCTGGACACCGCGCTGGCCCTGACCTTGCCTCCTGCCTTGACA TTGACGAGTGTCGTGAACGGGGACCTGCCCTGTGCGGGTCTCAGCGCTGTGAAAATTCTCCTGGTTCCTA CCGCTGTGTGCGGGACTGCGATCCAGGTTACCACCCCGGCCCTGAGGGCACCTGTGATGACATTGATGAG TGCCGAGAATACGGCTCTGCGATTTGTGGTGCCCAGCGCTGTGAGAACACCCCGGGCTCCTACCGCTGCA CACCAGCCTGCGACCCTGGCTATCAGCCTACACCAGGGGGCGGGTGCCAAGATGTGGATGAATGCCGGAA CCGGTCTTTCTGCGGCGCCCATGCTATGTGCCAGAATCTGCCTGGGTCCTTTCAGTGCGTCTGTGACCAA GGCTACGAAGGGGCACGGGACGGACGTCACTGCGTGGATGTGAATGAGTGTGAGACACTACAGGGCGTGT GTGGCTCCGCGCTGTGTGAAAATGTTGAAGGCTCCTTCCTGTGTGTCTGCCCCAATAGCCCTGAGGAATT TGATCCCATGACTGGACGCTGTGTTCCCCCACGGGCTCCTGCAGGCACGTTCCCTGGCTCCCAGCCCCAA GCACCTGCCAGCCCCAGTCTTCCAGCCAGGCCACCAGCCCCTCCCCCACCTCGAAGACCCAGCCCTCCCA GGCAGGGTCCCGTGAGCAGTGGACGTCGCGAGTGCTACTTTGATACAGCCGCTCCAGATGCGTGTGACAA TATCTTGGCCCGAAACGTGACGTGGCAGGAGTGCTGCTGTACTGTGGGAGAAGGCTGGGGCAGCGGCTGC CGCATCCAACAGTGCCCGGGCACCGAGACAGCTGAATACCAGTCATTGTGTCCTCACGGCCGGGGCTACC TGGTGCCCAGTGGAGACCTGAGTGCCCGGAGAGATGTGGACGAATGCCAACTCTTCCAGGACCAGGTGTG CAAGAGCGGAGTGTGTGTGAACACGGCCCCAGGCTACTCTTGCTATTGCAGCAACGGGTTCTACTATCAC GCACACAGGCTGGAGTGCGTTGATAATGACGAATGTGCGGACGAGGAGCCTGCTTGTGAAGGTGGCCGCT GCGTCAACACGGTGGGCTCTTACCACTGTACCTGCGAGCCCCCACTGGTGCTGGACGGCTCCCGGCGACG CTGCGTCTCCAACGAGAGCCAGAGCCTGGATGACAATCTAGGAGTGTGCTGGCAGGAAGTGGGACCTGAT CTCGTCTGCAGTCGCCCACGACTAGACCGGCAGGCCACCTACACCGAGTGCTGCTGTCTCTATGGTGAAG CCTGGGGTATGGACTGCGCTCTCTGTCCTGCCCAGGACTCAGATGACTTTGAGGCCCTTTGCAATGTGCT GCGTCCACCTGCCTATGGCCCGCCGCGCCCTGGTGGCTTTGGAATTCCCTATGAATATGGCCCAGATATA GGCCCACCTTATCAGAGTCTCCCCTATGGGCCAGATCTGTATCCACCGCCAGTGCTACCCTACGACCCCT ACCCACCCCCACCTGGACCCTTTGCTCGCCGGGAGGCCCCTTATGGCGCCCCACCCTTCGACATGCCGGA CTTTGAGGATGATGGTGGCCCCTATGGCGAGTCTGAGACTCCTGACCCACCTAGCCGAGGCACTGGTTGG CCTTATCGGTCCAGAGACACCCGTGGCTCCTTCCCAGAGCCTGAGGAATCCTCTGAGCGTGGAAGCTATA CAGGAGCCTTGTCTGAGCCCTATGAGGGCCTAGAGGCCGAAGAGTGTGGTATCCTGGATGGCTGCCCTCA CGGCCGCTGTGTGCGCGTTCCAGAAGGCTTCACCTGCGATTGTTTCGATGGCTACCGCCTGGACATCACC CGCATGTCCTGTGTCGACGTCAACGAGTGTGATGAGGCAGAGGCAACCTCCCCACTCTGCGTCAATGCGC GCTGTGTCAACACTGACGGCTCCTTCCGCTGTATCTGTCGTCCAGGATTTGCTCCCACGCATCAGCCACA TCACTGTGCGCCTGCTCGACCCCGGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222718 representing NM_175641 Red=Cloning site Green=Tags(s) MRRPGLGGPCPLLLLLLLPAATSASGSSPSPSPSPIEKAVVPSHQAGVAACHCCLDQTPKSSRCTRASCR VRNCPPAKCTGLEGCLTPTPSVPSPSRSPVEKSQVSLNWQPLTLQEARALLRQRRPRGPWARALLKRRPP HRAPAGQARVLCPLICHNGGVCVKPDRCLCPPDFAGKFCQLHSSGARPPAPAMPGLTRSVYTMPLANHRD DEHGVASMVSVHVEHPQEASVVVHQVERVSGPWEEANPEALARAEAAARAEAAAPYTVLAQSAPREDGYS DASGFGYCFRELRGSECASPLPGLRTQEVCCRGEGLAWGVHDCHPCAEHLRNSNQVSGPNGPCPPGFERV NGSCVDVDECATGGRCQHGECANTRGGYTCVCPDGFLLDSSRSSCISQHVISEAKGPCYRVLHDGGCSLP ILRNITKQICCCSRVGKAWGRGCQLCPPYGSEGFREICPAGPGYHYSASDLRYNTRPLNQDPPRVTFNQP RVPPATPRPPTGFLPTRRPEPRPDPGPQPEPRPRPEPRPRPESRPRPEPRPRPEPRPQPESQPRPESRPR PESQPWPEFPLPSIPAWTGPEIPESGPSSSMCQRNPQVCGPGRCVPRPSGYTCACDPGFRLGPQGTRCID IDECRRVPTPCAPGRCENTPGSFRCVCGTGFQAGPRATECLDVDECRRVPPPCDRGRCENTPGSFLCVCP AGYQAAPHGASCQDVDECTQSPGLCGRGVCENLPGSFRCVCPAGFRGSACEEDVDECAQQPPPCGPGRCD NTAGSFHCACPAGFRSRGPGAPCQDVDECSRSPSPCAYGRCENTEGSFKCVCPTGFQPNAAGSECEDVDE CENRLACPGQECVNSPGSFQCRACPVGHHLHRGRCTDVDECSSGTPCGLHGQCTNTKGSFHCSCSTGYRA PSGQPGPCADINECLEGDFCFPHGECLNTDGSFTCTCAPGYRPGPRGASCLDVDECSEEDLCQSGICTNT DGSFECICPPGHRAGPDLASCLDIDECRERGPALCGSQRCENSPGSYRCVRDCDPGYHPGPEGTCDDIDE CREYGSAICGAQRCENTPGSYRCTPACDPGYQPTPGGGCQDVDECRNRSFCGAHAMCQNLPGSFQCVCDQ GYEGARDGRHCVDVNECETLQGVCGSALCENVEGSFLCVCPNSPEEFDPMTGRCVPPRAPAGTFPGSQPQ APASPSLPARPPAPPPPRRPSPPRQGPVSSGRRECYFDTAAPDACDNILARNVTWQECCCTVGEGWGSGC RIQQCPGTETAEYQSLCPHGRGYLVPSGDLSARRDVDECQLFQDQVCKSGVCVNTAPGYSCYCSNGFYYH AHRLECVDNDECADEEPACEGGRCVNTVGSYHCTCEPPLVLDGSRRRCVSNESQSLDDNLGVCWQEVGPD LVCSRPRLDRQATYTECCCLYGEAWGMDCALCPAQDSDDFEALCNVLRPPAYGPPRPGGFGIPYEYGPDI GPPYQSLPYGPDLYPPPVLPYDPYPPPPGPFARREAPYGAPPFDMPDFEDDGGPYGESETPDPPSRGTGW PYRSRDTRGSFPEPEESSERGSYTGALSEPYEGLEAEECGILDGCPHGRCVRVPEGFTCDCFDGYRLDIT RMSCVDVNECDEAEATSPLCVNARCVNTDGSFRCICRPGFAPTHQPHHCAPARPRA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_175641
ORF Size	4998 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175641.2, NP_783572.2
RefSeq Size	5322 bp
RefSeq ORF	5001 bp
Locus ID	108075
UniProt ID	Q8K4G1
MW	178.6 kDa
Gene Summary	Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta.[UniProtKB/Swiss-Prot Function]

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