Notch4 (NM_010929) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Int-3; Int3; N4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223369 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCCCAGTTGCTGCTGCTGCTGCTCTTGCCACTCAATTTCCCTGTCATCCTGACCAGAGAGCTTC TGTGTGGAGGATCCCCAGAGCCCTGTGCCAACGGAGGCACCTGCCTGAGGCTATCTCGGGGACAAGGGAT CTGCCAGTGTGCCCCTGGATTTCTGGGTGAGACTTGCCAGTTTCCTGACCCCTGCAGGGATACCCAACTC TGCAAGAATGGTGGCAGCTGCCAAGCCCTGCTCCCCACACCCCCAAGCTCCCGTAGTCCTACTTCTCCAC TGACCCCTCACTTCTCCTGCACCTGCCCCTCTGGCTTCACCGGTGATCGATGCCAAACCCATCTGGAAGA GCTCTGTCCACCTTCTTTCTGTTCCAACGGGGGTCACTGCTATGTTCAGGCCTCAGGCCGCCCACAGTGC TCCTGCGAGCCTGGGTGGACAGGTGAGCAATGCCAGCTCCGAGACTTCTGCTCAGCCAACCCCTGTGCCA ACGGAGGCGTGTGCCTGGCCACATACCCCCAGATCCAGTGCCGCTGTCCACCTGGGTTCGAGGGTCACAC CTGTGAACGCGACATCAACGAGTGCTTCCTGGAGCCGGGACCCTGCCCTCAGGGCACCTCCTGCCATAAC ACCTTGGGTTCCTACCAGTGTCTCTGCCCTGTGGGGCAGGAAGGTCCCCAGTGCAAGCTCAGGAAGGGAG CCTGCCCTCCTGGAAGCTGTCTCAATGGGGGCACCTGCCAGCTGGTCCCAGAGGGACACTCCACCTTTCA TCTCTGCCTCTGTCCCCCAGGTTTCACGGGGCTGGACTGTGAGATGAACCCAGATGACTGTGTCAGGCAC CAGTGTCAGAACGGGGCCACCTGTCTGGATGGGCTGGACACCTACACCTGCCTCTGCCCCAAGACATGGA AGGGCTGGGACTGCTCTGAAGATATAGATGAATGTGAAGCCCGGGGTCCCCCTCGCTGCAGGAACGGTGG CACCTGCCAGAACACAGCTGGCAGCTTTCACTGTGTGTGCGTGAGTGGCTGGGGAGGCGCAGGCTGTGAG GAGAACCTGGATGACTGTGCAGCTGCCACCTGTGCCCCGGGATCCACCTGCATCGACCGTGTGGGCTCCT TCTCCTGCCTCTGCCCACCTGGACGCACAGGCCTCCTGTGCCACCTGGAAGACATGTGTTTGAGTCAGCC GTGCCACGTGAATGCCCAGTGCAGCACCAACCCTCTGACAGGCTCCACCCTCTGCATATGCCAGCCTGGC TACTCAGGATCCACCTGTCACCAAGATCTGGATGAGTGCCAAATGGCCCAGCAAGGACCCAGTCCCTGCG AACATGGCGGCTCCTGCATCAACACCCCTGGCTCCTTCAACTGCCTCTGCCTGCCTGGTTACACGGGCTC CCGCTGTGAAGCTGACCACAATGAGTGCCTGTCACAGCCCTGCCACCCAGGCAGCACCTGCCTGGACCTG CTTGCAACCTTCCACTGCCTCTGCCCACCAGGCTTGGAAGGGAGGCTCTGTGAGGTGGAGGTCAATGAGT GCACCTCTAATCCCTGCCTGAACCAAGCTGCCTGCCATGACCTGCTCAACGGCTTCCAGTGCCTCTGCCT TCCTGGATTCACCGGCGCCCGATGTGAGAAAGACATGGACGAGTGTAGCAGCACCCCCTGTGCCAATGGG GGGCGCTGCCGAGACCAGCCTGGAGCCTTCTACTGCGAGTGTCTCCCAGGCTTTGAAGGGCCACACTGTG AGAAAGAAGTGGACGAATGTCTGAGTGACCCCTGCCCCGTGGGAGCCAGCTGCCTTGATCTCCCCGGAGC ATTCTTCTGCCTCTGCCGTCCTGGTTTCACAGGTCAACTTTGTGAGGTTCCCTTGTGCACCCCCAACATG TGCCAACCTGGACAGCAATGCCAAGGTCAGGAACACAGAGCCCCCTGCCTCTGCCCTGACGGAAGTCCTG GCTGTGTTCCTGCCGAGGACAACTGCCCCTGTCACCATGGCCATTGCCAGAGATCCTTGTGTGTGTGTGA TGAGGGCTGGACTGGACCAGAATGCGAGACAGAACTGGGTGGCTGCATCTCCACACCCTGTGCCCATGGG GGGACCTGCCACCCACAGCCATCTGGCTACAACTGTACCTGCCCTGCAGGCTACATGGGGTTGACCTGTA GTGAGGAGGTGACAGCTTGTCACTCAGGGCCCTGTCTCAATGGTGGCTCCTGCAGCATCCGTCCTGAGGG CTATTCCTGCACCTGCCTTCCAAGTCACACAGGTCGCCACTGCCAGACTGCCGTGGACCACTGTGTGTCT GCCTCGTGCCTCAATGGGGGTACCTGTGTGAACAAGCCTGGCACTTTCTTCTGCCTCTGTGCCACTGGCT TCCAGGGGCTGCACTGTGAGGAGAAGACTAACCCCAGCTGTGCAGACAGCCCCTGCAGGAACAAGGCAAC CTGCCAAGACACACCTCGAGGGGCCCGCTGCCTCTGCAGCCCTGGCTATACAGGAAGCAGCTGCCAGACT CTGATAGACTTGTGTGCCCGGAAGCCCTGTCCACACACTGCTCGATGCCTCCAGAGTGGGCCCTCGTTCC AGTGCCTGTGCCTCCAGGGATGGACAGGGGCTCTCTGTGACTTCCCACTGTCCTGCCAGAAGGCCGCGAT GAGCCAAGGCATAGAGATCTCTGGCCTGTGCCAGAATGGAGGCCTCTGTATTGACACGGGCTCCTCCTAT TTCTGCCGCTGCCCTCCTGGATTCCAAGGCAAGTTATGCCAGGATAATGTGAACCCCTGTGAGCCCAATC CCTGCCATCACGGGTCTACCTGTGTGCCTCAGCCCAGTGGCTATGTCTGCCAGTGTGCCCCAGGCTATGA GGGACAGAACTGCTCAAAAGTACTTGACGCTTGTCAGTCCCAGCCCTGCCACAACCACGGAACCTGTACC TCCAGGCCTGGAGGCTTCCACTGTGCCTGCCCTCCAGGCTTCGTGGGACTGCGCTGTGAGGGAGATGTGG ATGAGTGTCTGGACCGGCCCTGTCACCCCTCGGGCACTGCAGCTTGCCACTCTTTAGCCAACGCCTTCTA CTGCCAGTGTCTGCCTGGGCACACAGGCCAGCGGTGTGAGGTGGAGATGGACCTCTGTCAGAGCCAACCC TGCTCCAATGGAGGATCCTGTGAGATCACAACAGGGCCACCCCCTGGCTTCACCTGTCACTGCCCCAAGG GTTTTGAAGGCCCCACCTGCAGCCACAAAGCCCTTTCCTGCGGCATCCATCACTGCCACAATGGAGGCCT ATGTCTGCCCTCCCCTAAGCCAGGGTCACCACCACTCTGTGCCTGCCTCAGTGGTTTTGGGGGCCCTGAC TGTCTGACACCTCCAGCTCCACCGGGCTGCGGTCCCCCCTCACCCTGCCTGCACAATGGTACCTGCACTG AGACCCCTGGGTTGGGCAACCCGGGCTTTCAATGCACCTGCCCTCCTGACTCTCCAGGGCCCCGGTGTCA AAGGCCAGGGGCAAGTGGGTGTGAGGGCCGAGGTGGTGATGGGACCTGCGATGCTGGCTGCAGTGGCCCA GGAGGAGACTGGGATGGAGGGGACTGTTCCCTGGGGGTCCCAGACCCCTGGAAGGGCTGTCCCCCGCATT CCCAGTGCTGGCTTCTGTTCCGGGACGGACGGTGTCACCCGCAGTGTGACTCTGAGGAGTGTCTCTTTGA TGGCTACGACTGTGAAATCCCTCTAACCTGCATCCCAGCCTATGACCAGTACTGCCGAGATCACTTCCAC AACGGGCACTGTGAGAAAGGCTGCAATAACGCTGAATGTGGCTGGGACGGGGGAGACTGCAGACCAGAAG GGGAAGACTCAGAGGGGAGGCCCTCCCTGGCCCTGCTGGTGGTGCTGAGGCCCCCAGCCCTGGATCAGCA GCTGCTTGCCCTGGCACGAGTGCTGTCCCTGACTCTGAGGGTCGGTCTCTGGGTGAGGAAGGACAGTGAA GGCAGGAACATGGTGTTCCCCTATCCTGGGACCCGGGCCAAAGAGGAGCTGAGTGGAGCTAGGGATTCCT CTTCATGGGAAAGACAAGCCCCTCCCACTCAGCCCCTGGGCAAGGAGACAGAGTCTCTTGGTGCAGGGTT TGTGGTAGTGATGGGAGTGGATCTGTCCCGCTGTGGTCCGGAACATCCTGCGTCCCGCTGCCCCTGGGAC TCTGGACTCCTGCTGCGCTTCCTTGCAGCAATGGCAGCAGTGGGAGCTCTGGAGCCCCTGCTGCCTGGAC CCTTGCTGGCGGCTCACCCTCAAGCAGGGACCAGGCCCCCTGCCAACCAGCTTCCCTGGCCCATTCTATG TTCACCAGTGGTTGGGGTGCTTCTCCTGGCCCTTGGGGCCCTTCTCGTCCTCCAGCTCATTCGGCGCCGG CGACGAGAACATGGGGCCCTGTGGCTGCCCCCTGGTTTCATTCGAAGGCCTCAGGCACAGCAGGCACCCC ACCGGCGGAGGCCCCCACTGGGCGAGGACAACATTGGTCTTAAGGCACTGAAGCCAGAGGCCGAAGTGGA TGAGGATGGAGTGGCCATGTGCTCGGGCCCTGAAGAGGGAGAGGCTGAAGAAACAGCCTCAGCCTCCAGG TGCCAGCTTTGGCCGCTCAACAGCAGCTGTGGAGAGCTCCCCCAGGCAGCCATGCTGACCCCTCCTCAGG AGTGTGAATCGGAGGTTCTGGATGTGGACACCTGTGGACCTGATGGGGTGACACCCCTGATGTCAGCCGT CTTCTGTGGGGGAGTGCAGTCCACGACTGGGGCTAGTCCACAGAGACTGGGGCTAGGAAATCTGGAACCC TGGGAACCACTGCTGGATAGAGGGGCCTGCCCCCAGGCTCACACTGTGGGCACTGGAGAGACGCCTCTGC ACCTAGCTGCCAGATTCTCTCGGCCAACCGCTGCCCGTCGCCTTCTTGAGGCTGGAGCCAACCCCAATCA GCCAGACCGCGCTGGGCGCACCCCACTTCACACTGCTGTGGCTGCCGACGCTCGGGAGGTTTGCCAGCTC CTATTGGCCAGCAGACAGACTTCGGTGGACGCCCGCACAGAGGACGGGACTACACCTTTGATGCTGGCTG CCAGGCTGGCCGTGGAGGACCTGGTTGAAGAATTGATCGCAGCCCGAGCAGATGTAGGAGCCAGGGATAA AAGGGGAAAAACTGCACTGCACTGGGCCGCTGCTGTGAACAACGCCCGAGCCGCCCGCTCTCTCCTCCAG GCTGGAGCGGATAAAGATGCCCAGGACAGTAGGGAACAGACGCCGCTTTTCCTGGCAGCGCGCGAAGGAG CCGTGGAGGTGGCGCAGCTGTTGCTGGAGCTCGGGGCGGCCCGGGGACTGCGAGACCAGGCCGGGCTGGC CCCAGGAGATGTGGCCCGCCAGCGCAGTCACTGGGACCTGCTAACGCTGCTGGAAGGGGCTGGACCGACT ACGCAGGAGGCCCGTGCGCACGCACGCACCACGCCGGGGGGCGGGGCCGCGCCGCGCTGCCGGACGCTGT CTGCGGGAGCGCGCCCGCGCGGGGGCGGAGCCTGTCTGCAGGCTCGCACTTGGTCGGTGGACTTGGGAGC GCGCGGAGGGAAGGTGTATGCTCGCTGCCGGAGCCGATCTGGAAGCTGCGGAGGCCCCACCACGCGCGGC CGCAGGTTCTCCGCGGGCTCCCGTGGACGACGCGGGGCTAGGGCATCACAGGATGACTGGCCTCGCGACT GGGTGGCCCTGGAAGCCTGCGGCTCCGCCTGCAGTGCGCCGATCCCGCCTCCCAGCCTGACCCCGTCCCC AGAACGTGGATCCCCTCAAGTTGCCTGGGGTCTTCCAGTTCACCAAGAGATTCCCTTAAACTCGGTTGTA AGAAATCTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223369 protein sequence Red=Cloning site Green=Tags(s) MQPQLLLLLLLPLNFPVILTRELLCGGSPEPCANGGTCLRLSRGQGICQCAPGFLGETCQFPDPCRDTQL CKNGGSCQALLPTPPSSRSPTSPLTPHFSCTCPSGFTGDRCQTHLEELCPPSFCSNGGHCYVQASGRPQC SCEPGWTGEQCQLRDFCSANPCANGGVCLATYPQIQCRCPPGFEGHTCERDINECFLEPGPCPQGTSCHN TLGSYQCLCPVGQEGPQCKLRKGACPPGSCLNGGTCQLVPEGHSTFHLCLCPPGFTGLDCEMNPDDCVRH QCQNGATCLDGLDTYTCLCPKTWKGWDCSEDIDECEARGPPRCRNGGTCQNTAGSFHCVCVSGWGGAGCE ENLDDCAAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHVNAQCSTNPLTGSTLCICQPG YSGSTCHQDLDECQMAQQGPSPCEHGGSCINTPGSFNCLCLPGYTGSRCEADHNECLSQPCHPGSTCLDL LATFHCLCPPGLEGRLCEVEVNECTSNPCLNQAACHDLLNGFQCLCLPGFTGARCEKDMDECSSTPCANG GRCRDQPGAFYCECLPGFEGPHCEKEVDECLSDPCPVGASCLDLPGAFFCLCRPGFTGQLCEVPLCTPNM CQPGQQCQGQEHRAPCLCPDGSPGCVPAEDNCPCHHGHCQRSLCVCDEGWTGPECETELGGCISTPCAHG GTCHPQPSGYNCTCPAGYMGLTCSEEVTACHSGPCLNGGSCSIRPEGYSCTCLPSHTGRHCQTAVDHCVS ASCLNGGTCVNKPGTFFCLCATGFQGLHCEEKTNPSCADSPCRNKATCQDTPRGARCLCSPGYTGSSCQT LIDLCARKPCPHTARCLQSGPSFQCLCLQGWTGALCDFPLSCQKAAMSQGIEISGLCQNGGLCIDTGSSY FCRCPPGFQGKLCQDNVNPCEPNPCHHGSTCVPQPSGYVCQCAPGYEGQNCSKVLDACQSQPCHNHGTCT SRPGGFHCACPPGFVGLRCEGDVDECLDRPCHPSGTAACHSLANAFYCQCLPGHTGQRCEVEMDLCQSQP CSNGGSCEITTGPPPGFTCHCPKGFEGPTCSHKALSCGIHHCHNGGLCLPSPKPGSPPLCACLSGFGGPD CLTPPAPPGCGPPSPCLHNGTCTETPGLGNPGFQCTCPPDSPGPRCQRPGASGCEGRGGDGTCDAGCSGP GGDWDGGDCSLGVPDPWKGCPPHSQCWLLFRDGRCHPQCDSEECLFDGYDCEIPLTCIPAYDQYCRDHFH NGHCEKGCNNAECGWDGGDCRPEGEDSEGRPSLALLVVLRPPALDQQLLALARVLSLTLRVGLWVRKDSE GRNMVFPYPGTRAKEELSGARDSSSWERQAPPTQPLGKETESLGAGFVVVMGVDLSRCGPEHPASRCPWD SGLLLRFLAAMAAVGALEPLLPGPLLAAHPQAGTRPPANQLPWPILCSPVVGVLLLALGALLVLQLIRRR RREHGALWLPPGFIRRPQAQQAPHRRRPPLGEDNIGLKALKPEAEVDEDGVAMCSGPEEGEAEETASASR CQLWPLNSSCGELPQAAMLTPPQECESEVLDVDTCGPDGVTPLMSAVFCGGVQSTTGASPQRLGLGNLEP WEPLLDRGACPQAHTVGTGETPLHLAARFSRPTAARRLLEAGANPNQPDRAGRTPLHTAVAADAREVCQL LLASRQTSVDARTEDGTTPLMLAARLAVEDLVEELIAARADVGARDKRGKTALHWAAAVNNARAARSLLQ AGADKDAQDSREQTPLFLAAREGAVEVAQLLLELGAARGLRDQAGLAPGDVARQRSHWDLLTLLEGAGPT TQEARAHARTTPGGGAAPRCRTLSAGARPRGGGACLQARTWSVDLGARGGKVYARCRSRSGSCGGPTTRG RRFSAGSRGRRGARASQDDWPRDWVALEACGSACSAPIPPPSLTPSPERGSPQVAWGLPVHQEIPLNSVV RNLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_010929
ORF Size	5895 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_010929.2, NP_035059.2
RefSeq Size	6604 bp
RefSeq ORF	5895 bp
Locus ID	18132
UniProt ID	P31695
MW	206.7 kDa
Gene Summary	Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system.[UniProtKB/Swiss-Prot Function]

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