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Pik3cb (NM_029094) Mouse Tagged ORF Clone
CAT＃: MR223909
- TrueORF®
Pik3cb (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb)
ORF Plasmid: tGFP
"NM_029094" in other vectors (4)
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CNY 7,408.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110001J02Rik; AI447572; p110beta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223909 representing NM_029094
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCTGCTATGGCAGACAACCTTGACATCTGGGCAGTGGACTCACAGATTGCATCCGATGGCGCCA TATCCGTCGATTTCCTTCTGCCCACCGGGATTTATATCCAGTTGGAAGTACCTCGGGAAGCTACCATTTC TTATATTAAACAGATGTTATGGAAGCAAGTTCACAACTACCCGATGTTTAACCTCCTCATGGACATTGAC TCGTATATGTTTGCATGTGTGAATCAAACTGCTGTATATGAGGAACTGGAAGACGAAACACGAAGACTTT GTGATGTCAGACCTTTTCTTCCAGTTCTCAAACTAGTGACTAGAAGCTGTGACCCCGCAGAAAAATTGGA CTCAAAAATTGGGGTTCTTATAGGAAAAGGTCTTCATGAGTTTGATGCCTTGAAGGATCCCGAAGTGAAT GAATTTAGAAGAAAAATGCGCAAATTCAGTGAGGCCAAGATTCAGTCTCTGGTAGGGTTGTCTTGGATCG ACTGGCTAAAGCACACGTATCCGCCTGAGCACGAGCCGTCCGTCCTGGAGAACTTGGAAGATAAACTTTA TGGAGGAAAGCTGGTTGTGGCTGTGCACTTTGAAAATAGCCAGGATGTATTTAGTTTTCAAGTGTCTCCC AATTTGAATCCTATAAAAATAAATGAATTGGCAATCCAGAAACGCCTCACTATTCGTGGAAAGGAAGATG AAGCTAGCCCCTGTGACTATGTGTTACAGGTCAGTGGGAGAGTGGAGTATGTGTTTGGCGATCATCCACT AATTCAGTTCCAGTACATCCGGAATTGTGTGATGAATAGAACCCTGCCCCACTTCATCCTTGTGGAATGT TGTAAGATCAAGAAAATGTATGAACAAGAAATGATTGCCATAGAGGCTGCCATCAACCGAAACTCATCCA ACCTTCCTCTCCCTTTACCACCAAAGAAAACGCGAGTTATTTCTCATATCTGGGACAACAACAACCCTTT CCAAATTACCTTGGTTAAAGGAAATAAGCTTAATACAGAAGAAACTGTGAAAGTTCATGTCCGAGCTGGG CTTTTTCACGGAACCGAGCTCCTGTGTAAAACCGTCGTAAGCTCAGAGATATCAGGAAAGAACGACCATA TTTGGAATGAACAACTGGAATTTGATATTAATATTTGTGACTTACCAAGAATGGCTCGATTATGTTTTGC TGTTTATGCAGTTTTGGATAAAGTAAAAACGAAGAAATCAACAAAGACTATTAATCCCTCTAAGTATCAG ACCATCAGGAAAGCCGGGAAAGTGCATTATCCTGTCGCATGGGTAAATACCATGGTTTTTGACTTCAAAG GACAGCTGAGGTCTGGAGACGTCATATTGCATAGCTGGTCTTCGTTTCCTGATGAGCTGGAAGAAATGCT GAATCCCATGGGGACTGTGCAGACGAACCCATATGCTGAGAACGCCACCGCCTTGCACATTACGTTCCCA GAGAATAAGAAGCAGCCGTGTTATTATCCCCCCTTCGATAAGATCATTGAGAAGGCAGCTGAGCTTGCCA GCGGAGACAGTGCTAATGTGTCAAGTCGTGGTGGAAAAAAATTTCTTGCTGTGCTGAAAGAAATCTTGGA CAGGGACCCCCTGTCTCAGCTGTGTGAGAACGAAATGGACCTTATTTGGACTCTACGGCAAGACTGCCGA GAAAATTTCCCTCAGTCACTGCCAAAACTACTCTTGTCAATCAAGTGGAATAAACTTGAAGATGTTGCTC AGCTTCAGGCGCTCCTGCAGATATGGCCCAAACTGCCCCCCAGGGAAGCCCTGGAACTCCTGGATTTCAA CTATCCAGACCAGTATGTCCGGGAATACGCTGTAGGCTGCCTTCGACAGATGAGTGATGAAGAACTCTCT CAGTATCTTTTACAATTGGTGCAAGTTTTGAAATATGAGCCTTTTCTCGATTGTGCCCTCTCCAGATTCC TATTAGAAAGAGCACTTGATAATCGGAGGATTGGGCAGTTTCTGTTTTGGCATCTTAGGTCAGAGGTGCA CACTCCTGCTGTGTCCGTACAGTTTGGTGTCATCCTGGAAGCATACTGTCGAGGAAGCGTGGGGCACATG AAAGTGCTTTCCAAACAGGTGGAAGCACTCAATAAGTTAAAAACTTTAAATAGCTTAATCAAACTGAATG CGGTGAAGCTGAGCAGAGCTAAGGGAAAGGAGGCCATGCACACGTGCCTGAAACAGAGTGCTTACCGGGA GGCGCTCTCTGACCTGCAGTCGCCGCTGAACCCCTGCGTCATCCTCTCAGAGCTCTATGTTGAAAAGTGC AAATACATGGACTCCAAGATGAAGCCCCTGTGGCTGGTCTACAGCAGCAGAGCCTTTGGAGAGGACTCGG TTGGAGTGATCTTTAAAAATGGTGACGATTTGCGGCAGGACATGCTGACGCTGCAGATGTTGCGCCTGAT GGATCTGCTTTGGAAAGAAGCTGGCTTGGACCTGCGGATGCTCCCCTATGGCTGCTTAGCAACAGGAGAT CGCTCTGGCCTCATTGAGGTTGTGAGCACCTCTGAGACAATCGCTGACATTCAGCTGAACAGTAGTAACG TGGCTGCCACGGCAGCCTTCAACAAAGACGCACTCCTGAACTGGCTCAAGGAGTACAACTCTGGGGATGA CCTGGACCGAGCGATTGAGGAGTTTACCTTGTCCTGTGCTGGCTACTGTGTAGCCTCTTATGTCCTCGGC ATTGGTGACAGGCACAGTGACAACATCATGGTGAAGAAAACCGGCCAGCTCTTCCACATAGATTTTGGGC ATATTCTTGGAAATTTCAAATCTAAATTTGGCATTAAAAGGGAGCGAGTACCTTTTATTCTTACTTATGA CTTCATTCATGTCATTCAACAAGGAAAAACGGGAAACACTGAAAAATTTGGCAGATTCCGCCAGTGCTGT GAAGATGCGTATCTGATTTTACGGCGGCATGGGAATCTCTTCATCACCCTGTTTGCCCTGATGTTGACTG CAGGGCTGCCTGAGCTCACATCGGTCAAAGATATACAGTATCTTAAGGACTCGCTTGCCTTAGGGAAGAG CGAGGAGGAAGCACTGAAGCAGTTCAAGCAGAAGTTTGACGAGGCCCTCAGGGAAAGCTGGACTACTAAA GTGAACTGGATGGCTCACACAGTACGGAAAGACTACAGGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223909 representing NM_029094
Red=Cloning site Green=Tags(s) MPPAMADNLDIWAVDSQIASDGAISVDFLLPTGIYIQLEVPREATISYIKQMLWKQVHNYPMFNLLMDID SYMFACVNQTAVYEELEDETRRLCDVRPFLPVLKLVTRSCDPAEKLDSKIGVLIGKGLHEFDALKDPEVN EFRRKMRKFSEAKIQSLVGLSWIDWLKHTYPPEHEPSVLENLEDKLYGGKLVVAVHFENSQDVFSFQVSP NLNPIKINELAIQKRLTIRGKEDEASPCDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNRTLPHFILVEC CKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRVISHIWDNNNPFQITLVKGNKLNTEETVKVHVRAG LFHGTELLCKTVVSSEISGKNDHIWNEQLEFDINICDLPRMARLCFAVYAVLDKVKTKKSTKTINPSKYQ TIRKAGKVHYPVAWVNTMVFDFKGQLRSGDVILHSWSSFPDELEEMLNPMGTVQTNPYAENATALHITFP ENKKQPCYYPPFDKIIEKAAELASGDSANVSSRGGKKFLAVLKEILDRDPLSQLCENEMDLIWTLRQDCR ENFPQSLPKLLLSIKWNKLEDVAQLQALLQIWPKLPPREALELLDFNYPDQYVREYAVGCLRQMSDEELS QYLLQLVQVLKYEPFLDCALSRFLLERALDNRRIGQFLFWHLRSEVHTPAVSVQFGVILEAYCRGSVGHM KVLSKQVEALNKLKTLNSLIKLNAVKLSRAKGKEAMHTCLKQSAYREALSDLQSPLNPCVILSELYVEKC KYMDSKMKPLWLVYSSRAFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGD RSGLIEVVSTSETIADIQLNSSNVAATAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLG IGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCC EDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQKFDEALRESWTTK VNWMAHTVRKDYRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029094 |
| ORF Size | 3192 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029094.3, NP_083370.2 |
| RefSeq Size | 4854 bp |
| RefSeq ORF | 3195 bp |
| Locus ID | 74769 |
| UniProt ID | Q8BTI9 |
| MW | 122.2 kDa |
| Gene Summary | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223419 | Pik3cb (untagged) - Mouse phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb), (10ug) |
CNY 13,680.00 |
|
| MG223909 | Pik3cb (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase catalytic beta polypeptide (Pik3cb), (10ug) |
CNY 9,220.00 |
|
| MR223909L3 | Lenti ORF clone of Pik3cb (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb) |
CNY 10,260.00 |
|
| MR223909L4 | Lenti ORF clone of Pik3cb (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb) |
CNY 10,260.00 |
