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Ifi35 (NM_027320) Mouse Tagged ORF Clone
CAT＃: MR223920
- TrueORF®
Ifi35 (Myc-DDK-tagged) - Mouse interferon-induced protein 35 (Ifi35)
ORF Plasmid: tGFP
"NM_027320" in other vectors (4)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010008K16Rik; AW986054; ifi-35; IFP35 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223920 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTGACCCTGCAAACTGTCCTCTACAGTCTTCAGGAGGAGCAAGCCAGGCTCAAGATGAGGCTGC AGGAGCTGCAGCAGCTCAAAAGGGAGCGCACAGGCTCTCCCGGAGCCAAGATCCCATTCTCAGTACCTGA AGTTCCTCTGGTATTCCAAGGCCAAACTAAGCAGGGCAGGCAAGTGCCCAAGTTTGTAGTTTCTAACTTG AAGGTCTGCTGCCCTCTGCCTGAAGGTTCTGCTCTGGTCACCTTTGAGGACCCCAAAGTGGTTGATCGGT TGCTACAACAAAAGGAACACAGAGTTAACTTAGAGGACTGCTGGCTGCGGGTGCAGGTCCAGCCCTTGGA GCTGCCTGTGGTGACCAACATTCAGGTGTCCAGCCAGCCAGATAACCACAGGGTGCTCGTTAGTGGTTTT CCTGCTGGACTTAGGCTGAGTGAAGAGGAACTGTTGGACAAGCTGGAGATCTTCTTTGGCAAGGCCAAGA ATGGAGGTGGGGATGTAGAGACCCGGGAGATGCTGCAAGGGACCGTCATGCTAGGGTTTGCTGATGAAGA AGTGGCCCAGCACTTATGCCAGATTGGCCAGTTCAGAGTCCCACTGGACCGGCAGCAGGTCCTCCTGAGG GTCTCTCCCTATGTGAGTGGTGAGATCCAGAAAGCCGAGATCAAATTCCAGCAAGCCCCTCATTCAGTGC TGGTGACAAATATTCCTGATGTCATGGATGCCCAGGAACTGCATGACATCCTTGAGATCCACTTCCAGAA GCCCACTCGTGGGGGCGGGGAGGTGGAGGCCCTGACAGTTGTGCCTTCAGGGCAGCAGGGCCTGGCTATC TTCACTTCCGAGTCAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223920 protein sequence
Red=Cloning site Green=Tags(s) MSVTLQTVLYSLQEEQARLKMRLQELQQLKRERTGSPGAKIPFSVPEVPLVFQGQTKQGRQVPKFVVSNL KVCCPLPEGSALVTFEDPKVVDRLLQQKEHRVNLEDCWLRVQVQPLELPVVTNIQVSSQPDNHRVLVSGF PAGLRLSEEELLDKLEIFFGKAKNGGGDVETREMLQGTVMLGFADEEVAQHLCQIGQFRVPLDRQQVLLR VSPYVSGEIQKAEIKFQQAPHSVLVTNIPDVMDAQELHDILEIHFQKPTRGGGEVEALTVVPSGQQGLAI FTSESS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027320 |
| ORF Size | 861 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027320.1, NM_027320.2, NM_027320.3, NM_027320.4, NP_081596.1 |
| RefSeq Size | 1360 bp |
| RefSeq ORF | 861 bp |
| Locus ID | 70110 |
| UniProt ID | Q9D8C4 |
| MW | 31.9 kDa |
| Gene Summary | Acts as a signaling pathway regulator involved in innate immune system response (PubMed:29350881). In response to interferon IFN-alpha, associates in a complex with transcriptional regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (By similarity). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (By similarity). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (By similarity). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211040 | Ifi35 (untagged) - Mouse interferon-induced protein 35 (Ifi35), (10ug) |
CNY 3,990.00 |
|
| MG223920 | Ifi35 (tGFP-tagged) - Mouse interferon-induced protein 35 (Ifi35), (10ug) |
CNY 2,850.00 |
|
| MR223920L3 | Lenti ORF clone of Ifi35 (Myc-DDK-tagged) - Mouse interferon-induced protein 35 (Ifi35) |
CNY 4,750.00 |
|
| MR223920L4 | Lenti ORF clone of Ifi35 (mGFP-tagged) - Mouse interferon-induced protein 35 (Ifi35) |
CNY 4,750.00 |
