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Nsun7 (NM_027602) Mouse Tagged ORF Clone
CAT＃: MR224506
- TrueORF®
Nsun7 (Myc-DDK-tagged) - Mouse NOL1/NOP2/Sun domain family, member 7 (Nsun7)
ORF Plasmid: tGFP
"NM_027602" in other vectors (4)
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CNY 5,128.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4921525L17Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224506 representing NM_027602
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGACCCAACGAGTGAAAGGGACCTTTTTGACCAAGAAAACATGGAGGAGATCTCCCAACTCGCTT CCCTGGAGATGTCGGGGGATGTAGTCGCCAATACAAACTCTTCAACCGTCGTCCTAGAAAAACCCAGCTA CCCAGACTCCGTGTACGTTACGGCAGCGAACATTTTTCAGGGTATTCGGATCCAAAGGTCGCCAGACAAA GTCATAATAAATTACGGAAGTGAACCCCTGCAGCCCTCCTCCTCCCGCTCCGAGGATGAATCTTTCCAGC GCTTGTCCTATGAGTTGGCTTTCAGTACCCTGAAGTATCAAGATATTTTGGAAAGTATATTAATAGATAG CTATATCTTCTCAAGTACCACAATCGCAAGTCAGCTGAACAGTCTTATCATTGTGATGCTGTATGACTTC CAAGACAGAAAATTCCAGCCCCGCATCCTTTCCGAGAATGAAGAGACCATACCAGAAGTTCAAGAAGTCG AGAACCTTCTAAACGGCTTTAAGACAAAATTGGCTGCAGCCTTGGCGAGATGTCGAATCAAACACGACGC CCTCTCGATTTACCATATTCTGCCAGAGACAGTTAGGAAACAGGAACAAAGGGCCTCAACTTTGCCTCTT TATGCTTGGATAAATACCAGTAAAATAAGCCTTGAAGAAGTCTATAATAATTTGAGGAGGAAAGGTTACT CTAAAGTCAAGTCTATAACGAGTGTCAATGAGAAAGTCTATGCAGTAGACCAGCACTGCTTCAATGTCTT AATTTTCCCAGCTCATCTCAAAACAGACCTTTTAAACATAGATCTCATAAAAGACTATAAACTTATTTTC CAGGACAAATCTCGAAGTCTTGCCGTCCACTCTGTGAAGGCTTTGATAAACATCGATGATGATGTCCTAA TGGTCAACACGGGTTCGTGGTACACAGTTGCCCATATGTCCATCTTAACAAGTGGTCACACTTCGAAAAT CTTTGTATGTGGAATACAACAAGAAGAGAAGGATTTTAATGCGAGGAAACTTTTTACAAGAATGGGATGT CAAAATATTGAAATACTTCACGAAACATTTCTTAGCATTGAATCCAAAGATCACCGGTTACAGAATGTGA AGGTCATTCTGTTGCTACCTCGCTGTTCCAGCCTCGGTGTCAGCAACCCAGTGGAATTCATTCTAAATGA ACACGAAGATAAAAGTTTACTTCAAGATCTTTCTCAAGGTGGTCTACCGAAAGATAAACTCGAGACTTTG GTTCAGCAGCAGTTTGAACAGCTAACACACGCAATGAAATTTACCAAAGTTCAAGCAATTGTCTATTGCA CATGTTCTGTTTCTAAAGAGGAAAATGAAGACGTTGTTGAGAAAGCACTGGAATATCAAAGCTCAGGGGT TAAAATGCAACCTTACAGACTCAGTCCTCCTGTTCTCCCACTGTGCACCCTAAAAGAGATAGAACTGTCT ATGGATAGATTCTTCAGATTGGAACCATCTGATATGAACAATGGCTGTTTTCTTTCTATCTTAACAAGAG AGCGGGACCCATCTGAGACAGTGTCAGTGAAAGACGTTCTGGCCAGGGCAGCAGCCAAAGGTCTACTGGA AGGGGTTGAGGTTGGGAAGACGCTCAAAAGGGACAAGAAGAGGAAAAAATCGAAAGCACTGCCATCCAGG GCTCCTCACCATGGGGACCCACTTCGCGACCATTTGGCAGTGGATGGTAACGACACCAGCAACGTCCAAA TGAAAATCTCCGAGCTCTTGCACCGAGAAAGCAAGATAAGTACTTCAACAAAAATGTCCGCCCCAGCAAA GACTGTGTCTCAAGCCGGCACTTCCTCCCAAGTCAGAAAGCCCAGCAAGCCTTTATCGACCCCACTGGTG AGGAATTTTTCCAGGCCGGTAGAAAGGCCAACGAACTTTGTGAGAGCGCGGCCAGAAGGCAAAGTGATTC CTTTGAAACCAATTGAAATCGTTCTGCCGCCAGTGATATTCCCATTGAGTTCCCAGGGGCCCAGGGTTCA GATGCCAGCCACCCATTTTTATTACCGCTTCATTGGATCAAAGAGATTCTGCTCTTCTTTAGGACTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224506 representing NM_027602
Red=Cloning site Green=Tags(s) MLDPTSERDLFDQENMEEISQLASLEMSGDVVANTNSSTVVLEKPSYPDSVYVTAANIFQGIRIQRSPDK VIINYGSEPLQPSSSRSEDESFQRLSYELAFSTLKYQDILESILIDSYIFSSTTIASQLNSLIIVMLYDF QDRKFQPRILSENEETIPEVQEVENLLNGFKTKLAAALARCRIKHDALSIYHILPETVRKQEQRASTLPL YAWINTSKISLEEVYNNLRRKGYSKVKSITSVNEKVYAVDQHCFNVLIFPAHLKTDLLNIDLIKDYKLIF QDKSRSLAVHSVKALINIDDDVLMVNTGSWYTVAHMSILTSGHTSKIFVCGIQQEEKDFNARKLFTRMGC QNIEILHETFLSIESKDHRLQNVKVILLLPRCSSLGVSNPVEFILNEHEDKSLLQDLSQGGLPKDKLETL VQQQFEQLTHAMKFTKVQAIVYCTCSVSKEENEDVVEKALEYQSSGVKMQPYRLSPPVLPLCTLKEIELS MDRFFRLEPSDMNNGCFLSILTRERDPSETVSVKDVLARAAAKGLLEGVEVGKTLKRDKKRKKSKALPSR APHHGDPLRDHLAVDGNDTSNVQMKISELLHRESKISTSTKMSAPAKTVSQAGTSSQVRKPSKPLSTPLV RNFSRPVERPTNFVRARPEGKVIPLKPIEIVLPPVIFPLSSQGPRVQMPATHFYYRFIGSKRFCSSLGLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027602 |
| ORF Size | 2100 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027602.2, NP_081878.1 |
| RefSeq Size | 2552 bp |
| RefSeq ORF | 2103 bp |
| Locus ID | 70918 |
| UniProt ID | Q14AW5 |
| MW | 79.6 kDa |
| Gene Summary | This gene encodes a member of the NOL1/NOP2/sun domain RNA methyltransferase family. Mice with a mutation in this gene exhibit male sterility due to impaired sperm motility. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220595 | Nsun7 (untagged) - Mouse NOL1/NOP2/Sun domain family, member 7 (Nsun7), (10ug) |
CNY 9,030.00 |
|
| MG224506 | Nsun7 (tGFP-tagged) - Mouse NOL1/NOP2/Sun domain family member 7 (Nsun7), (10ug) |
CNY 6,370.00 |
|
| MR224506L3 | Lenti ORF clone of Nsun7 (Myc-DDK-tagged) - Mouse NOL1/NOP2/Sun domain family, member 7 (Nsun7) |
CNY 7,700.00 |
|
| MR224506L4 | Lenti ORF clone of Nsun7 (mGFP-tagged) - Mouse NOL1/NOP2/Sun domain family, member 7 (Nsun7) |
CNY 7,700.00 |
